LOCUS       sequence-137762-        9665 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses humanized MbCpf1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9665)
  AUTHORS   Zetsche B, Gootenberg JS, Abudayyeh OO, Slaymaker IM, Makarova KS, 
            Essletzbichler P, Volz SE, Joung J, van der Oost J, Regev A, Koonin 
            EV, Zhang F
  TITLE     Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas 
            System.
  JOURNAL   Cell. 2015 Sep 23. pii: S0092-8674(15)01200-3. doi: 
            10.1016/j.cell.2015.09.038.
  PUBMED    26422227
REFERENCE   2  (bases 1 to 9665)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2015 
            Sep 23. pii: S0092-8674(15)01200-3. doi: 
            10.1016/j.cell.2015.09.038."
COMMENT     Sequence Label: pY014 (pcDNA3.1-hMbCpf1)
FEATURES             Location/Qualifiers
     source          1..9665
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        12..391
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        392..595
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     546..566
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     640..659
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        640..658
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             4825..4872
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             4879..4959
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     primer_bind     complement(5033..5050)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5039..5263
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5309..5737
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5396..5415)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5606..5627
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5746..5766)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5751..6080
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5931..6066
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5993..6012
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6147..6941
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6201..6220)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6811..6830
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7115..7236
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7152..7171)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7206..7225
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7285..7301)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7285..7301)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7298..7320)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7309..7325
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7333..7339,7340..7357,7358..7363))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7378..7399
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7516..7533)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7687..8275)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7767..7786)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8446..9237,9238..9306))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9069..9088
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9307..9411)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9486..9505)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt
       61 agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg
      121 ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac
      181 gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt
      241 ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa
      301 atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta
      361 catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg
      421 gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg
      481 gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc
      541 attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctctctg
      601 gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc actataggga
      661 gacccaagct ggctagcgtt taaacttaag cttggtaccg ccaccatgct gttccaggac
      721 tttacccacc tgtatccact gtccaagaca gtgagatttg agctgaagcc catcgatagg
      781 accctggagc acatccacgc caagaacttc ctgtctcagg acgagacaat ggccgatatg
      841 caccagaagg tgaaagtgat cctggacgat taccaccgcg acttcatcgc cgatatgatg
      901 ggcgaggtga agctgaccaa gctggccgag ttctatgacg tgtacctgaa gtttcggaag
      961 aacccaaagg acgatgagct gcagaagcag ctgaaggatc tgcaggccgt gctgagaaag
     1021 gagatcgtga agcccatcgg caatggcggc aagtataagg ccggctacga caggctgttc
     1081 ggcgccaagc tgtttaagga cggcaaggag ctgggcgatc tggccaagtt cgtgatcgca
     1141 caggagggag agagctcccc aaagctggcc cacctggccc acttcgagaa gttttccacc
     1201 tatttcacag gctttcacga taaccggaag aatatgtatt ctgacgagga taagcacacc
     1261 gccatcgcct accgcctgat ccacgagaac ctgccccggt ttatcgacaa tctgcagatc
     1321 ctgaccacaa tcaagcagaa gcactctgcc ctgtacgatc agatcatcaa cgagctgacc
     1381 gccagcggcc tggacgtgtc tctggccagc cacctggatg gctatcacaa gctgctgaca
     1441 caggagggca tcaccgccta caatacactg ctgggaggaa tctccggaga ggcaggctct
     1501 cctaagatcc agggcatcaa cgagctgatc aattctcacc acaaccagca ctgccacaag
     1561 agcgagagaa tcgccaagct gaggccactg cacaagcaga tcctgtccga cggcatgagc
     1621 gtgtccttcc tgccctctaa gtttgccgac gatagcgaga tgtgccaggc cgtgaacgag
     1681 ttctatcgcc actacgccga cgtgttcgcc aaggtgcaga gcctgttcga cggctttgac
     1741 gatcaccaga aggatggcat ctacgtggag cacaagaacc tgaatgagct gtccaagcag
     1801 gccttcggcg actttgcact gctgggacgc gtgctggacg gatactatgt ggatgtggtg
     1861 aatccagagt tcaacgagcg gtttgccaag gccaagaccg acaatgccaa ggccaagctg
     1921 acaaaggaga aggataagtt catcaagggc gtgcactccc tggcctctct ggagcaggcc
     1981 atcgagcact ataccgcaag gcacgacgat gagagcgtgc aggcaggcaa gctgggacag
     2041 tacttcaagc acggcctggc cggagtggac aaccccatcc agaagatcca caacaatcac
     2101 agcaccatca agggctttct ggagagggag cgccctgcag gagagagagc cctgccaaag
     2161 atcaagtccg gcaagaatcc tgagatgaca cagctgaggc agctgaagga gctgctggat
     2221 aacgccctga atgtggccca cttcgccaag ctgctgacca caaagaccac actggacaat
     2281 caggatggca acttctatgg cgagtttggc gtgctgtacg acgagctggc caagatcccc
     2341 accctgtata acaaggtgag agattacctg agccagaagc ctttctccac cgagaagtac
     2401 aagctgaact ttggcaatcc aacactgctg aatggctggg acctgaacaa ggagaaggat
     2461 aatttcggcg tgatcctgca gaaggacggc tgctactatc tggccctgct ggacaaggcc
     2521 cacaagaagg tgtttgataa cgcccctaat acaggcaaga gcatctatca gaagatgatc
     2581 tataagtacc tggaggtgag gaagcagttc cccaaggtgt tcttttccaa ggaggccatc
     2641 gccatcaact accacccttc taaggagctg gtggagatca aggacaaggg ccggcagaga
     2701 tccgacgatg agcgcctgaa gctgtatcgg tttatcctgg agtgtctgaa gatccaccct
     2761 aagtacgata agaagttcga gggcgccatc ggcgacatcc agctgtttaa gaaggataag
     2821 aagggcagag aggtgccaat cagcgagaag gacctgttcg ataagatcaa cggcatcttt
     2881 tctagcaagc ctaagctgga gatggaggac ttctttatcg gcgagttcaa gaggtataac
     2941 ccaagccagg acctggtgga tcagtataat atctacaaga agatcgactc caacgataat
     3001 cgcaagaagg agaatttcta caacaatcac cccaagttta agaaggatct ggtgcggtac
     3061 tattacgagt ctatgtgcaa gcacgaggag tgggaggaga gcttcgagtt ttccaagaag
     3121 ctgcaggaca tcggctgtta cgtggatgtg aacgagctgt ttaccgagat cgagacacgg
     3181 agactgaatt ataagatctc cttctgcaac atcaatgccg actacatcga tgagctggtg
     3241 gagcagggcc agctgtatct gttccagatc tacaacaagg acttttcccc aaaggcccac
     3301 ggcaagccca atctgcacac cctgtacttc aaggccctgt tttctgagga caacctggcc
     3361 gatcctatct ataagctgaa tggcgaggcc cagatcttct acagaaaggc ctccctggac
     3421 atgaacgaga caacaatcca cagggccggc gaggtgctgg agaacaagaa tcccgataat
     3481 cctaagaaga gacagttcgt gtacgacatc atcaaggata agaggtacac acaggacaag
     3541 ttcatgctgc acgtgccaat caccatgaac tttggcgtgc agggcatgac aatcaaggag
     3601 ttcaataaga aggtgaacca gtctatccag cagtatgacg aggtgaacgt gatcggcatc
     3661 gatcggggcg agagacacct gctgtacctg accgtgatca atagcaaggg cgagatcctg
     3721 gagcagtgtt ccctgaacga catcaccaca gcctctgcca atggcacaca gatgaccaca
     3781 ccttaccaca agatcctgga taagagggag atcgagcgcc tgaacgcccg ggtgggatgg
     3841 ggcgagatcg agacaatcaa ggagctgaag tctggctatc tgagccacgt ggtgcaccag
     3901 atcagccagc tgatgctgaa gtacaacgcc atcgtggtgc tggaggacct gaatttcggc
     3961 tttaagaggg gccgctttaa ggtggagaag cagatctatc agaacttcga gaatgccctg
     4021 atcaagaagc tgaaccacct ggtgctgaag gacaaggccg acgatgagat cggctcttac
     4081 aagaatgccc tgcagctgac caacaatttc acagatctga agagcatcgg caagcagacc
     4141 ggcttcctgt tttatgtgcc cgcctggaac acctctaaga tcgaccctga gacaggcttt
     4201 gtggatctgc tgaagccaag atacgagaac atcgcccaga gccaggcctt ctttggcaag
     4261 ttcgacaaga tctgctataa tgccgacaag gattacttcg agtttcacat cgactacgcc
     4321 aagtttaccg ataaggccaa gaatagccgc cagatctgga caatctgttc ccacggcgac
     4381 aagcggtacg tgtacgataa gacagccaac cagaataagg gcgccgccaa gggcatcaac
     4441 gtgaatgatg agctgaagtc cctgttcgcc cgccaccaca tcaacgagaa gcagcccaac
     4501 ctggtcatgg acatctgcca gaacaatgat aaggagtttc acaagtctct gatgtacctg
     4561 ctgaaaaccc tgctggccct gcggtacagc aacgcctcct ctgacgagga tttcatcctg
     4621 tcccccgtgg caaacgacga gggcgtgttc tttaatagcg ccctggccga cgatacacag
     4681 cctcagaatg ccgatgccaa cggcgcctac cacatcgccc tgaagggcct gtggctgctg
     4741 aatgagctga agaactccga cgatctgaac aaggtgaagc tggccatcga caatcagacc
     4801 tggctgaatt tcgcccagaa caggaaaagg ccggcggcca cgaaaaaggc cggccaggca
     4861 aaaaagaaaa agggatccta cccatacgat gttccagatt acgcttatcc ctacgacgtg
     4921 cctgattatg catacccata tgatgtcccc gactatgcct aagaattctg cagatatcca
     4981 gcacagtggc ggccgctcga gtctagaggg cccgtttaaa cccgctgatc agcctcgact
     5041 gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg
     5101 gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg
     5161 agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg
     5221 gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga
     5281 accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt aagcgcggcg
     5341 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     5401 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     5461 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     5521 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     5581 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     5641 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     5701 aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt
     5761 tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     5821 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     5881 gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc
     5941 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     6001 cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg
     6061 gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc
     6121 aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc
     6181 cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct
     6241 ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg
     6301 acctgtccgg tgccctgaat gaactgcagg acgaggcagc gcggctatcg tggctggcca
     6361 cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc
     6421 tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga
     6481 aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc
     6541 cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc
     6601 ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg
     6661 ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct
     6721 gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc
     6781 tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc
     6841 ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc
     6901 agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcga
     6961 aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt
     7021 ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg
     7081 cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg
     7141 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     7201 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc
     7261 tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
     7321 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     7381 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     7441 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     7501 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     7561 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     7621 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     7681 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     7741 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     7801 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     7861 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     7921 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     7981 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     8041 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     8101 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     8161 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     8221 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     8281 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     8341 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     8401 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     8461 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     8521 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     8581 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     8641 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     8701 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     8761 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     8821 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     8881 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     8941 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     9001 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     9061 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     9121 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     9181 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     9241 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     9301 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     9361 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     9421 ccgaaaagtg ccacctgacg tcgacggatc gggagatctc ccgatcccct atggtgcact
     9481 ctcagtacaa tctgctctga tgccgcatag ttaagccagt atctgctccc tgcttgtgtg
     9541 ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta caacaaggca aggcttgacc
     9601 gacaattgca tgaagaatct gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg
     9661 gccag
//
