LOCUS       sequence-137763-       10141 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  lentiviral expression of EGFP and shRNA (from H1 promoter, empty).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10141)
  AUTHORS   Schluter OM, Xu W, Malenka RC
  TITLE     Alternative N-terminal domains of PSD-95 and SAP97 govern 
            activity-dependent regulation of synaptic AMPA receptor function.
  JOURNAL   Neuron. 2006 Jul 6;51(1):99-111.
  PUBMED    16815335
REFERENCE   2  (bases 1 to 10141)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron."; 
            date: "2006-07-6"; volume: "51(1)"; pages: "99-111"
COMMENT     Sequence Label: FHUG+W (B2)
FEATURES             Location/Qualifiers
     source          1..10141
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2611..2825
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     2746..2765
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     promoter        2869..3268
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3916..3935
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             join(4077..4079,4080..4082,4083..4796)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4122..4143)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4383..4402)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4730..4751
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    4839..5427
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4892..4912)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5310..5321)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5946..6126
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6152..6169)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6158..6382
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6428..6856
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6515..6534)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6725..6746
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6865..6885)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6870..7199
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7050..7185
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7112..7131
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7247..7294
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7313..7687
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7817..7938
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7854..7873)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7908..7927
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7987..8003)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7987..8003)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8000..8022)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8011..8027
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8035..8041,8042..8059,8060..8065))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8080..8101
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8218..8235)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8389..8977)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8469..8488)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9148..9939,9940..10008))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9771..9790
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10009..10113)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatcg tacgaattcg gaacgctgac gtcatcaacc cgctccaagg
     2641 aatcgcgggc ccagtgtcac taggcgggaa cacccagcgc gcgtgcgccc tggcaggaag
     2701 atggctgtga gggacagggg agtggcgccc tgcaatattt gcatgtcgct atgtgttctg
     2761 ggaaatcacc ataaacgtga aatgtctttg gatttgggaa tcttataagt tctgtatgag
     2821 accacggcgc gccccagcta gctggttcga attaattaag ggtgcagcgg cctccgcgcc
     2881 gggttttggc gcctcccgcg ggcgcccccc tcctcacggc gagcgctgcc acgtcagacg
     2941 aagggcgcag gagcgttcct gatccttccg cccggacgct caggacagcg gcccgctgct
     3001 cataagactc ggccttagaa ccccagtatc agcagaagga cattttagga cgggacttgg
     3061 gtgactctag ggcactggtt ttctttccag agagcggaac aggcgaggaa aagtagtccc
     3121 ttctcggcga ttctgcggag ggatctccgt ggggcggtga acgccgatga ttatataagg
     3181 acgcgccggg tgtggcacag ctagttccgt cgcagccggg atttgggtcg cggttcttgt
     3241 ttgtggatcg ctgtgatcgt cacttggtga gttgcgggct gctgggctgg ccggggcttt
     3301 cgtggccgcc gggccgctcg gtgggacgga agcgtgtgga gagaccgcca agggctgtag
     3361 tctgggtccg cgagcaaggt tgccctgaac tgggggttgg ggggagcgca caaaatggcg
     3421 gctgttcccg agtcttgaat ggaagacgct tgtaaggcgg gctgtgaggt cgttgaaaca
     3481 aggtgggggg catggtgggc ggcaagaacc caaggtcttg aggccttcgc taatgcggga
     3541 aagctcttat tcgggtgaga tgggctgggg caccatctgg ggaccctgac gtgaagtttg
     3601 tcactgactg gagaactcgg gtttgtcgtc tggttgcggg ggcggcagtt atgcggtgcc
     3661 gttgggcagt gcacccgtac ctttgggagc gcgcgcctcg tcgtgtcgtg acgtcacccg
     3721 ttctgttggc ttataatgca gggtggggcc acctgccggt aggtgtgcgg taggcttttc
     3781 tccgtcgcag gacgcagggt tcgggcctag ggtaggctct cctgaatcga caggcgccgg
     3841 acctctggtg aggggaggga taagtgaggc gtcagtttct ttggtcggtt ttatgtacct
     3901 atcttcttaa gtagctgaag ctccggtttt gaactatgcg ctcggggttg gcgagtgtgt
     3961 tttgtgaagt tttttaggca ccttttgaaa tgtaatcatt tgggtcaata tgtaattttc
     4021 agtgttagac tagtaaagct tctgcaggtc gactctagag gatccaccgg tccaccatgg
     4081 tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg
     4141 acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca
     4201 agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg
     4261 tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc
     4321 acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca
     4381 aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga
     4441 accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc
     4501 tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca
     4561 tcaaggcgaa cttcaagatc cgccacaaca tcgaggacgg cggcgtgcag ctcgccgacc
     4621 actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc
     4681 tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc
     4741 tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtaagcta
     4801 gctcgagcta gaattaattc gatatcaagc ttatcgataa tcaacctctg gattacaaaa
     4861 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg
     4921 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct
     4981 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg
     5041 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct
     5101 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg
     5161 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg
     5221 tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc
     5281 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc
     5341 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc
     5401 ggatctccct ttgggccgcc tccccgcatc gataccgtcg agacctagaa aaacatggag
     5461 caatcacaag tagcaataca gcagctacca atgctgattg tgcctggcta gaagcacaag
     5521 aggaggagga ggtgggtttt ccagtcacac ctcaggtacc tttaagacca atgacttaca
     5581 aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc
     5641 actcccaacg aagacaagat atccttgatc tgtggatcta ccacacacaa ggctacttcc
     5701 ctgattggca gaactacaca ccagggccag ggatcagata tccactgacc tttggatggt
     5761 gctacaagct agtaccagtt gagcaagaga aggtagaaga agccaatgaa ggagagaaca
     5821 cccgcttgtt acaccctgtg agcctgcatg ggatggatga cccggagaga gaagtattag
     5881 agtggaggtt tgacagccgc ctagcatttc atcacatggc ccgagagctg catccggact
     5941 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
     6001 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
     6061 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     6121 ctagcagggc ccgtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca
     6181 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc
     6241 ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg
     6301 gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct
     6361 ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg
     6421 tatccccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     6481 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     6541 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     6601 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
     6661 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     6721 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
     6781 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
     6841 aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag
     6901 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
     6961 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     7021 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     7081 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg
     7141 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
     7201 agctcccggg agcttgtata tccattttcg gatctgatca gcacgtgttg acaattaatc
     7261 atcggcatag tatatcggca tagtataata cgacaaggtg aggaactaaa ccatggccaa
     7321 gttgaccagt gccgttccgg tgctcaccgc gcgcgacgtc gccggagcgg tcgagttctg
     7381 gaccgaccgg ctcgggttct cccgggactt cgtggaggac gacttcgccg gtgtggtccg
     7441 ggacgacgtg accctgttca tcagcgcggt ccaggaccag gtggtgccgg acaacaccct
     7501 ggcctgggtg tgggtgcgcg gcctggacga gctgtacgcc gagtggtcgg aggtcgtgtc
     7561 cacgaacttc cgggacgcct ccgggccggc catgaccgag atcggcgagc agccgtgggg
     7621 gcgggagttc gccctgcgcg acccggccgg caactgcgtg cacttcgtgg ccgaggagca
     7681 ggactgacac gtgctacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt
     7741 cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga
     7801 gttcttcgcc caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     7861 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     7921 actcatcaat gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta
     7981 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     8041 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     8101 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     8161 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     8221 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     8281 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     8341 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     8401 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     8461 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     8521 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     8581 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     8641 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     8701 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     8761 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     8821 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     8881 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     8941 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     9001 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     9061 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     9121 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     9181 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     9241 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     9301 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     9361 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     9421 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     9481 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     9541 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     9601 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     9661 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     9721 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     9781 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     9841 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     9901 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     9961 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
    10021 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
    10081 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
    10141 c
//
