LOCUS       sequence-137766-        9260 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses humanized CMtCpf1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9260)
  AUTHORS   Zetsche B, Gootenberg JS, Abudayyeh OO, Slaymaker IM, Makarova KS, 
            Essletzbichler P, Volz SE, Joung J, van der Oost J, Regev A, Koonin 
            EV, Zhang F
  TITLE     Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas 
            System.
  JOURNAL   Cell. 2015 Sep 23. pii: S0092-8674(15)01200-3. doi: 
            10.1016/j.cell.2015.09.038.
  PUBMED    26422227
REFERENCE   2  (bases 1 to 9260)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2015 
            Sep 23. pii: S0092-8674(15)01200-3. doi: 
            10.1016/j.cell.2015.09.038."
COMMENT     Sequence Label: pY012 (pcDNA3.1-hCMtCpf1)
FEATURES             Location/Qualifiers
     source          1..9260
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        15..394
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        395..598
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     549..569
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     643..662
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        643..661
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             4423..4470
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             4477..4557
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     primer_bind     complement(4631..4648)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4637..4861
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4907..5335
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4994..5013)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5204..5225
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5344..5364)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5349..5678
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5529..5664
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5591..5610
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5745..6539
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5799..5818)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6409..6428
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6713..6834
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6750..6769)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6804..6823
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6883..6899)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6883..6899)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6896..6918)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6907..6923
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6931..6937,6938..6955,6956..6961))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6976..6997
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7114..7131)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7285..7873)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7365..7384)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8044..8835,8836..8904))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8667..8686
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8905..9009)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9084..9103)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 cagatatacg cgttgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc
       61 attagttcat agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc
      121 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt
      181 aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca
      241 cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg
      301 taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca
      361 gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa
      421 tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa
      481 tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc
      541 cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctct
      601 ctggctaact agagaaccca ctgcttactg gcttatcgaa attaatacga ctcactatag
      661 ggagacccaa gctggctagc gtttaaactt aagcttggta ccgccaccat gaacaattac
      721 gacgagttca ccaagctgta tcctatccag aaaaccatcc ggtttgagct gaagccacag
      781 ggcagaacca tggagcacct ggagacattc aacttctttg aggaggaccg ggatagagcc
      841 gagaagtata agatcctgaa ggaggccatc gacgagtacc acaagaagtt tatcgatgag
      901 cacctgacca atatgtccct ggattggaac tctctgaagc agatcagcga gaagtactat
      961 aagagcaggg aggagaagga caagaaggtg ttcctgtccg agcagaagag gatgcgccag
     1021 gagatcgtgt ctgagtttaa gaaggacgat cgcttcaagg acctgttttc caagaagctg
     1081 ttctctgagc tgctgaagga ggagatctac aagaagggca accaccagga gatcgacgcc
     1141 ctgaagagct tcgataagtt ttccggctat ttcatcggcc tgcacgagaa taggaagaac
     1201 atgtactccg acggcgatga gatcaccgcc atctccaatc gcatcgtgaa tgagaacttc
     1261 cccaagtttc tggataacct gcagaagtac caggaggcca ggaagaagta tcctgagtgg
     1321 atcatcaagg ccgagagcgc cctggtggcc cacaatatca agatggacga ggtgttctcc
     1381 ctggagtact ttaataaggt gctgaaccag gagggcatcc agcggtacaa cctggccctg
     1441 ggcggctatg tgaccaagag cggcgagaag atgatgggcc tgaatgatgc cctgaacctg
     1501 gcccaccagt ccgagaagag ctccaagggc agaatccaca tgacccccct gttcaagcag
     1561 atcctgtccg agaaggagtc cttctcttac atccccgacg tgtttacaga ggattctcag
     1621 ctgctgccta gcatcggcgg cttctttgcc cagatcgaga atgacaagga tggcaacatc
     1681 ttcgaccggg ccctggagct gatctctagc tacgccgagt atgataccga gcggatctat
     1741 atcagacagg ccgacatcaa tagagtgtcc aacgtgatct ttggagagtg gggcaccctg
     1801 ggaggcctga tgagggagta caaggccgac tctatcaatg atatcaacct ggagcgcaca
     1861 tgcaagaagg tggacaagtg gctggattct aaggagtttg ccctgagcga tgtgctggag
     1921 gccatcaaga ggaccggcaa caatgacgcc ttcaacgagt atatctccaa gatgcggaca
     1981 gccagagaga agatcgatgc cgcccgcaag gagatgaagt tcatcagcga gaagatctcc
     2041 ggcgatgagg agtctatcca catcatcaag accctgctgg acagcgtgca gcagttcctg
     2101 cacttcttta atctgtttaa ggcaaggcag gacatcccac tggatggagc cttctacgcc
     2161 gagtttgacg aggtgcacag caagctgttt gccatcgtgc ccctgtataa caaggtgcgg
     2221 aactatctga ccaagaacaa tctgaacaca aagaagatca agctgaattt caagaaccct
     2281 acactggcca atggctggga ccagaacaag gtgtacgatt atgcctccct gatctttctg
     2341 cgggacggca attactatct gggcatcatc aatcctaaga gaaagaagaa catcaagttc
     2401 gagcagggct ctggcaacgg ccccttctac cggaagatgg tgtataagca gatccccggc
     2461 cctaataaga acctgccaag agtgttcctg acctccacaa agggcaagaa ggagtataag
     2521 ccctctaagg agatcatcga gggctacgag gccgacaagc acatcagggg cgataagttc
     2581 gacctggatt tttgtcacaa gctgatcgat ttctttaagg agtccatcga gaagcacaag
     2641 gactggtcta agttcaactt ctacttcagc ccaaccgaga gctatggcga catctctgag
     2701 ttctacctgg atgtggagaa gcagggctat cgcatgcact ttgagaatat cagcgccgag
     2761 acaatcgacg agtatgtgga gaagggcgat ctgtttctgt tccagatcta caacaaggat
     2821 tttgtgaagg ccgccaccgg caagaaggac atgcacacaa tctactggaa tgccgccttc
     2881 agccccgaga acctgcagga cgtggtggtg aagctgaacg gcgaggccga gctgttttat
     2941 agggacaagt ccgatatcaa ggagatcgtg caccgcgagg gcgagatcct ggtgaatagg
     3001 acctacaacg gccgcacacc agtgcccgac aagatccaca agaagctgac cgattatcac
     3061 aatggccgga caaaggacct gggcgaggcc aaggagtacc tggataaggt gagatacttc
     3121 aaggcccact atgacatcac caaggatcgg agatacctga acgacaagat ctatttccac
     3181 gtgcctctga ccctgaactt caaggccaac ggcaagaaga atctgaacaa gatggtcatc
     3241 gagaagttcc tgtccgatga gaaggcccac atcatcggca tcgacagggg cgagcgcaat
     3301 ctgctgtact attccatcat cgacaggtct ggcaagatca tcgatcagca gagcctgaat
     3361 gtgatcgacg gctttgatta tcgggagaag ctgaaccaga gagagatcga gatgaaggat
     3421 gcccgccagt cttggaacgc catcggcaag atcaaggacc tgaaggaggg ctacctgagc
     3481 aaggccgtgc acgagatcac caagatggcc atccagtata atgccatcgt ggtcatggag
     3541 gagctgaact acggcttcaa gcggggccgg ttcaaggtgg agaagcagat ctatcagaag
     3601 ttcgagaata tgctgatcga taagatgaac tacctggtgt ttaaggacgc acctgatgag
     3661 tccccaggag gcgtgctgaa tgcctaccag ctgacaaacc cactggagtc tttcgccaag
     3721 ctgggcaagc agaccggcat cctgttttac gtgccagccg cctatacatc caagatcgac
     3781 cccaccacag gcttcgtgaa tctgtttaac acctcctcta agacaaacgc ccaggagcgg
     3841 aaggagttcc tgcagaagtt tgagagcatc tcctattctg ccaaggatgg cggcatcttt
     3901 gccttcgcct ttgactacag aaagttcggc accagcaaga cagatcacaa gaacgtgtgg
     3961 accgcctata caaacggcga gaggatgcgc tacatcaagg agaagaagcg gaatgagctg
     4021 tttgaccctt ctaaggagat caaggaggcc ctgaccagct ccggcatcaa gtacgatggc
     4081 ggccagaaca tcctgccaga catcctgagg agcaacaata acggcctgat ctacacaatg
     4141 tattctagct tcatcgccgc catccagatg cgcgtgtacg acggcaagga ggattatatc
     4201 atcagcccca tcaagaactc caagggcgag ttctttagga ccgaccccaa gaggcgcgag
     4261 ctgcctatcg acgccgatgc caatggcgcc tacaacatcg ccctgagggg agagctgaca
     4321 atgagggcaa tcgcagagaa gttcgaccct gatagcgaga agatggccaa gctggagctg
     4381 aagcacaagg attggttcga gtttatgcag accagaggcg acaaaaggcc ggcggccacg
     4441 aaaaaggccg gccaggcaaa aaagaaaaag ggatcctacc catacgatgt tccagattac
     4501 gcttatccct acgacgtgcc tgattatgca tacccatatg atgtccccga ctatgcctaa
     4561 gaattctgca gatatccagc acagtggcgg ccgctcgagt ctagagggcc cgtttaaacc
     4621 cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc
     4681 gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa
     4741 attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac
     4801 agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg
     4861 gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc
     4921 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
     4981 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
     5041 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
     5101 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
     5161 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
     5221 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
     5281 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattaattc
     5341 tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta
     5401 tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag
     5461 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa
     5521 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     5581 taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt
     5641 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat
     5701 ccattttcgg atctgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg
     5761 gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac
     5821 aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg
     5881 ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaggac gaggcagcgc
     5941 ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg
     6001 aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc
     6061 accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc
     6121 ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta
     6181 ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg
     6241 cgccagccga actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg
     6301 tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat
     6361 tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc
     6421 gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta
     6481 tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag
     6541 cgggactctg gggttcgaaa tgaccgacca agcgacgccc aacctgccat cacgagattt
     6601 cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg
     6661 ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt
     6721 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     6781 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     6841 ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt
     6901 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
     6961 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
     7021 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
     7081 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     7141 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     7201 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     7261 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     7321 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     7381 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     7441 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     7501 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     7561 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     7621 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     7681 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     7741 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     7801 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     7861 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     7921 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     7981 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     8041 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     8101 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     8161 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     8221 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     8281 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     8341 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     8401 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     8461 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     8521 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     8581 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     8641 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     8701 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     8761 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     8821 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     8881 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     8941 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     9001 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc gacggatcgg gagatctccc
     9061 gatcccctat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat
     9121 ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca
     9181 acaaggcaag gcttgaccga caattgcatg aagaatctgc ttagggttag gcgttttgcg
     9241 ctgcttcgcg atgtacgggc
//
