LOCUS       sequence-137767-        9164 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses humanized Lb2Cpf1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9164)
  AUTHORS   Zetsche B, Gootenberg JS, Abudayyeh OO, Slaymaker IM, Makarova KS, 
            Essletzbichler P, Volz SE, Joung J, van der Oost J, Regev A, Koonin 
            EV, Zhang F
  TITLE     Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas 
            System.
  JOURNAL   Cell. 2015 Sep 23. pii: S0092-8674(15)01200-3. doi: 
            10.1016/j.cell.2015.09.038.
  PUBMED    26422227
REFERENCE   2  (bases 1 to 9164)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2015 
            Sep 23. pii: S0092-8674(15)01200-3. doi: 
            10.1016/j.cell.2015.09.038."
COMMENT     Sequence Label: pY011 (pcDNA3.1-hLb2Cpf1)
FEATURES             Location/Qualifiers
     source          1..9164
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        14..393
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        394..597
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     548..568
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     642..661
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        642..660
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             4326..4373
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             4380..4460
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     primer_bind     complement(4534..4551)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4540..4764
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4810..5238
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4897..4916)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5107..5128
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5247..5267)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5252..5581
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5432..5567
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5494..5513
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5648..6442
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5702..5721)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6312..6331
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6616..6737
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6653..6672)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6707..6726
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6786..6802)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6786..6802)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6799..6821)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6810..6826
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6834..6840,6841..6858,6859..6864))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6879..6900
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7017..7034)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7188..7776)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7268..7287)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7947..8738,8739..8807))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8570..8589
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8808..8912)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8987..9006)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 agatatacgc gttgacattg attattgact agttattaat agtaatcaat tacggggtca
       61 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct
      121 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
      181 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
      241 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt
      301 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag
      361 tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat
      421 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat
      481 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc
      541 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctctc
      601 tggctaacta gagaacccac tgcttactgg cttatcgaaa ttaatacgac tcactatagg
      661 gagacccaag ctggctagcg tttaaactta agcttggtac cgccaccatg tactatgagt
      721 ccctgaccaa gcagtacccc gtgtctaaga caatccggaa tgagctgatc cctatcggca
      781 agacactgga taacatccgc cagaacaata tcctggagag cgacgtgaag cggaagcaga
      841 actacgagca cgtgaagggc atcctggatg agtatcacaa gcagctgatc aacgaggccc
      901 tggacaattg caccctgcca tccctgaaga tcgccgccga gatctacctg aagaatcaga
      961 aggaggtgtc tgacagagag gatttcaaca agacacagga cctgctgagg aaggaggtgg
     1021 tggagaagct gaaggcccac gagaacttta ccaagatcgg caagaaggac atcctggatc
     1081 tgctggagaa gctgccttcc atctctgagg acgattacaa tgccctggag agcttccgca
     1141 acttttacac ctatttcaca tcctacaaca aggtgcggga gaatctgtat tctgataagg
     1201 agaagagctc cacagtggcc tacagactga tcaacgagaa tttcccaaag tttctggaca
     1261 atgtgaagag ctataggttt gtgaaaaccg caggcatcct ggcagatggc ctgggagagg
     1321 aggagcagga ctccctgttc atcgtggaga cattcaacaa gaccctgaca caggacggca
     1381 tcgataccta caattctcaa gtgggcaaga tcaactctag catcaatctg tataaccaga
     1441 agaatcagaa ggccaatggc ttcagaaaga tccccaagat gaagatgctg tataagcaga
     1501 tcctgtccga tagggaggag tctttcatcg acgagtttca gagcgatgag gtgctgatcg
     1561 acaacgtgga gtcttatggc agcgtgctga tcgagtctct gaagtcctct aaggtgagcg
     1621 ccttctttga tgccctgaga gagtctaagg gcaagaacgt gtacgtgaag aatgacctgg
     1681 ccaagacagc catgagcaac atcgtgttcg agaattggag gacctttgac gatctgctga
     1741 accaggagta cgacctggcc aacgagaaca agaagaagga cgataagtat ttcgagaagc
     1801 gccagaagga gctgaagaag aataagagct actccctgga gcacctgtgc aacctgtccg
     1861 aggattcttg taacctgatc gagaattata tccaccagat ctccgacgat atcgagaata
     1921 tcatcatcaa caatgagaca ttcctgcgca tcgtgatcaa tgagcacgac aggtcccgca
     1981 agctggccaa gaaccggaag gccgtgaagg ccatcaagga ctttctggat tctatcaagg
     2041 tgctggagcg ggagctgaag ctgatcaaca gctccggcca ggagctggag aaggatctga
     2101 tcgtgtactc tgcccacgag gagctgctgg tggagctgaa gcaggtggac agcctgtata
     2161 acatgaccag aaattatctg acaaagaagc ctttctctac cgagaaggtg aagctgaact
     2221 ttaatcgcag cacactgctg aacggctggg atcggaataa ggagacagac aacctgggcg
     2281 tgctgctgct gaaggacggc aagtactatc tgggcatcat gaacacaagc gccaataagg
     2341 ccttcgtgaa tccccctgtg gccaagaccg agaaggtgtt taagaaggtg gattacaagc
     2401 tgctgccagt gcccaaccag atgctgccaa aggtgttctt tgccaagagc aatatcgact
     2461 tctataaccc ctctagcgag atctactcca attataagaa gggcacccac aagaagggca
     2521 atatgttttc cctggaggat tgtcacaacc tgatcgactt ctttaaggag tctatcagca
     2581 agcacgagga ctggagcaag ttcggcttta agttcagcga tacagcctcc tacaacgaca
     2641 tctccgagtt ctatcgcgag gtggagaagc agggctacaa gctgacctat acagacatcg
     2701 atgagacata catcaatgat ctgatcgagc ggaacgagct gtacctgttc cagatctata
     2761 ataaggactt tagcatgtac tccaagggca agctgaacct gcacacactg tatttcatga
     2821 tgctgtttga tcagcgcaat atcgacgacg tggtgtataa gctgaacgga gaggcagagg
     2881 tgttctatag gccagcctcc atctctgagg acgagctgat catccacaag gccggcgagg
     2941 agatcaagaa caagaatcct aaccgggcca gaaccaagga gacaagcacc ttcagctacg
     3001 acatcgtgaa ggataagcgg tatagcaagg ataagtttac cctgcacatc cccatcacaa
     3061 tgaacttcgg cgtggatgag gtgaagcggt tcaacgacgc cgtgaacagc gccatccgga
     3121 tcgatgagaa tgtgaacgtg atcggcatcg accggggcga gagaaatctg ctgtacgtgg
     3181 tggtcatcga ctctaagggc aacatcctgg agcagatctc cctgaactct atcatcaata
     3241 aggagtacga catcgagaca gattatcacg cactgctgga tgagagggag ggcggcagag
     3301 ataaggcccg gaaggactgg aacaccgtgg agaatatcag ggacctgaag gccggctacc
     3361 tgagccaggt ggtgaacgtg gtggccaagc tggtgctgaa gtataatgcc atcatctgcc
     3421 tggaggacct gaactttggc ttcaagaggg gccgccagaa ggtggagaag caggtgtacc
     3481 agaagttcga gaagatgctg atcgataagc tgaattacct ggtcatcgac aagagccgcg
     3541 agcagacatc ccctaaggag ctgggaggcg ccctgaacgc actgcagctg acctctaagt
     3601 tcaagagctt taaggagctg ggcaagcagt ccggcgtgat ctactatgtg cctgcctacc
     3661 tgacctctaa gatcgatcca accacaggct tcgccaatct gttttatatg aagtgtgaga
     3721 acgtggagaa gtccaagaga ttctttgacg gctttgattt catcaggttc aacgccctgg
     3781 agaacgtgtt cgagttcggc tttgactacc ggagcttcac ccagagggcc tgcggcatca
     3841 attccaagtg gaccgtgtgc accaacggcg agcgcatcat caagtatcgg aatccagata
     3901 agaacaatat gttcgacgag aaggtggtgg tggtgaccga tgagatgaag aacctgtttg
     3961 agcagtacaa gatcccctat gaggatggca gaaatgtgaa ggacatgatc atcagcaacg
     4021 aggaggccga gttctaccgg agactgtata ggctgctgca gcagaccctg cagatgagaa
     4081 acagcacctc cgacggcaca agggattaca tcatctcccc tgtgaagaat aagagagagg
     4141 cctacttcaa cagcgagctg tccgacggct ctgtgccaaa ggacgccgat gccaacggcg
     4201 cctacaatat cgccagaaag ggcctgtggg tgctggagca gatcaggcag aagagcgagg
     4261 gcgagaagat caatctggcc atgaccaacg ccgagtggct ggagtatgcc cagacacacc
     4321 tgctgaaaag gccggcggcc acgaaaaagg ccggccaggc aaaaaagaaa aagggatcct
     4381 acccatacga tgttccagat tacgcttatc cctacgacgt gcctgattat gcatacccat
     4441 atgatgtccc cgactatgcc taagaattct gcagatatcc agcacagtgg cggccgctcg
     4501 agtctagagg gcccgtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc
     4561 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg
     4621 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc
     4681 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg
     4741 ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg
     4801 ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
     4861 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
     4921 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
     4981 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
     5041 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
     5101 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
     5161 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
     5221 aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc
     5281 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg
     5341 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     5401 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     5461 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     5521 tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
     5581 aaagctcccg ggagcttgta tatccatttt cggatctgat caagagacag gatgaggatc
     5641 gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag
     5701 gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg
     5761 gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa
     5821 tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc
     5881 agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc
     5941 ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga
     6001 tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa
     6061 acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct
     6121 ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgcgcat
     6181 gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt
     6241 ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta
     6301 tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga
     6361 ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg
     6421 ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga ccaagcgacg
     6481 cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc
     6541 ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag
     6601 ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     6661 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     6721 ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag cttggcgtaa
     6781 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     6841 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
     6901 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     6961 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     7021 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     7081 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     7141 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     7201 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     7261 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     7321 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     7381 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     7441 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     7501 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     7561 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     7621 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     7681 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     7741 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     7801 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     7861 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     7921 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     7981 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     8041 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     8101 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     8161 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     8221 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     8281 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     8341 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     8401 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     8461 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     8521 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     8581 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     8641 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     8701 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     8761 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     8821 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     8881 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     8941 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
     9001 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
     9061 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
     9121 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggcc
//
