LOCUS       sequence-137781-         820 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway-compatible sucrose counter-selection allelic exchange vector
            for Pseudomonas syringae.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 820)
  TITLE     Kvitko lab Gateway compatible allelic exchange plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pDEST2T18ms
FEATURES             Location/Qualifiers
     source          1..820
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    17..141
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(166..171,172..189,190..196)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(316..335)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 cctcgggtat gcatgcacaa gtttgtacaa aaaagctgaa cgagaaacgt aaaatgatat
       61 aaatatcaat atattaaatt agattttgca taaaaaacag actacataat actgtaaaac
      121 acaacatatc cagtcactat ggcggccgca ttaggcaccc caggctttac actttatgct
      181 tccggctcgt ataatgtgtg gattttgagt taggatccgt cgagattttc aggagctaag
      241 gaagctaaaa tggagaaaaa aatcactgga tataccaccg ttgatatatc ccaatggcat
      301 cgtaaagaac attttgaggc atttcagtca gttgctcaat gtacctataa ccagaccgtt
      361 cagctggata ttacggcctt tttaaagacc gtaaagaaaa ataagcacaa gttttatccg
      421 gcctttattc acattcttgc ccgcctgatg aatgctcatc cggaattccg tatggcaatg
      481 aaagacggtg agctggtgat atgggatagt gttcaccctt gttacaccgt tttccatgag
      541 caaactgaaa cgttttcatc gctctggagt gaataccacg acgatttccg gcagtttcta
      601 cacatatatt cgcaagatgt ggcgtgttac ggtgaaaacc tggcctatct ccctaaaggg
      661 tttattgaga atatgttttt cgtctcagcc aatccctggg tgagtttcac cagttttgat
      721 ttaaacgtgg ccaatatgga caacttcttc gcccccgttt tcaccatggg caaatattat
      781 acgcaaggcg acaaggtgct gatgccgctg gcgattcagg
//
