LOCUS       sequence-137784-         781 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of myc-tagged mRAP1 with single point mutatoin I312R.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 781)
  AUTHORS   Yeung F, Ramirez CM, Mateos-Gomez PA, Pinzaru A, Ceccarini G, Kabir 
            S, Fernandez-Hernando C, Sfeir A
  TITLE     Nontelomeric role for Rap1 in regulating metabolism and protecting 
            against obesity.
  JOURNAL   Cell Rep. 2013 Jun 27;3(6):1847-56. doi: 
            10.1016/j.celrep.2013.05.032. Epub 2013 Jun 20.
  PUBMED    23791522
REFERENCE   2  (bases 1 to 781)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2013.05.032"; journalName: "Cell Rep"; date: 
            "2013-06-27- 27"; volume: "3"; issue: "6"; pages: "1847-56"
COMMENT     Sequence Label: pLPC myc-mRAP1 I312R
FEATURES             Location/Qualifiers
     source          1..781
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     50..69
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        50..68
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      100..109
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             109..138
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 agagctctct ggctactaga gaacccactg cttactggct tatcgaaatt aatacgactc
       61 actataggga gacccaagct tggtaccgag ctcggatcag ccaccatgga acaaaagttg
      121 atttctgaag aagatttgtt aagaaagaga ggatccgcgg aggcgatgga tctgggtaaa
      181 gaccccaatg ggcccactca ctcctccact ctgttcgtga gagaagacgg cagcgccatg
      241 tcgttttacg tgcggcccag ctcggccaag cgccggctgt cgacgctcat cctgcacggc
      301 ggcggcaccg tgtgtcgggt gcaggaaccc ggagccgtgc ttctcgccca gcccggggag
      361 gcgctggccg aggcttcggg cgacttcatc tccacgcagt acatcctaga ctgcgtggat
      421 cgcaacgaga agctggacct ggaggcctat cggctgggcc tgacggagca ggcgtccgat
      481 ccgaagcccg gggcttccac cgagggctcc acggaaccgg agccgcagcc cctgaccggg
      541 cgcatcgcct acaccgacgc ggaagatgtg gccatcctga cctacgtgaa ggagaacgcc
      601 cgttcgccca gctcggtcac aggcaatgcc ttgtggaaag cgatggagaa gagctcgctc
      661 acgcagcact cctggcagtc gctcaaggac cgctacctca agcacctacg gggtcaggag
      721 cacaagtacc tgctcgggaa cgccccggtc agcccgtcct cccagaagct caaacggaag
      781 g
//
