LOCUS       sequence-137785-         719 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of Flag-tagged mRAP1 with single point mutatoin I312R.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 719)
  AUTHORS   Yeung F, Ramirez CM, Mateos-Gomez PA, Pinzaru A, Ceccarini G, Kabir 
            S, Fernandez-Hernando C, Sfeir A
  TITLE     Nontelomeric role for Rap1 in regulating metabolism and protecting 
            against obesity.
  JOURNAL   Cell Rep. 2013 Jun 27;3(6):1847-56. doi: 
            10.1016/j.celrep.2013.05.032. Epub 2013 Jun 20.
  PUBMED    23791522
REFERENCE   2  (bases 1 to 719)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2013.05.032"; journalName: "Cell Rep"; date: 
            "2013-06-27- 27"; volume: "3"; issue: "6"; pages: "1847-56"
COMMENT     Sequence Label: pLPC flag-mRAP1 I312R
FEATURES             Location/Qualifiers
     source          1..719
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     20..39
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        20..38
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      70..79
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             79..102
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 cttactggct tatcgaaatt aatacgactc actataggga gacccaagct tggtaccgag
       61 ctcggatcag ccaccatgga ctacaaagac gatgacgata aaggatccgc ggaggcgatg
      121 gatctgggta aagaccccaa tgggcccact cactcctcca ctctgttcgt gagagaagac
      181 ggcagcgcca tgtcgtttta cgtgcggccc agctcggcca agcgccggct gtcgacgctc
      241 atcctgcacg gcggcggcac cgtgtgtcgg gtgcaggaac ccggagccgt gcttctcgcc
      301 cagcccgggg aggcgctggc cgaggcttcg ggcgacttca tctccacgca gtacatccta
      361 gactgcgtgg atcgcaacga gaagctggac ctggaggcct atcggctggg cctgacggag
      421 caggcgtccg atccgaagcc cggggcttcc accgagggct ccacggaacc ggagccgcag
      481 cccctgaccg ggcgcatcgc ctacaccgac gcggaagatg tggccatcct gacctacgtg
      541 aaggagaacg cccgttcgcc cagctcggtc acaggcaatg ccttgtggaa agcgatggag
      601 aagagctcgc tcacgcagca ctcctggcag tcgctcaagg accgctacct caagcaccta
      661 cggggtcagg agcacaagta cctgctcggg aacgccccgg tcagcccgtc ctcccagaa
//
