LOCUS       sequence-137788-         697 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression in Mammalian Cells, Cas9 Nuclease to analize 
            translocations.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 697)
  AUTHORS   Mateos-Gomez PA, Gong F, Nair N, Miller KM, Lazzerini-Denchi E, 
            Sfeir A
  TITLE     Mammalian polymerase theta promotes alternative NHEJ and suppresses 
            recombination.
  JOURNAL   Nature. 2015 Feb 12;518(7538):254-7. doi: 10.1038/nature14157. Epub 
            2015 Feb 2.
  PUBMED    25642960
REFERENCE   2  (bases 1 to 697)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature14157"; journalName: "Nature"; date: "2015-02-12- 
            12"; volume: "518"; issue: "7538"; pages: "254-7"
COMMENT     Sequence Label: pX330(puro) Rosa26-H3F3B
FEATURES             Location/Qualifiers
     source          1..697
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        36..114
                     /label=tracrRNA
                     /note="trans-activating CRISPR RNA for the Streptococcus 
                     pyogenes CRISPR/Cas9 system"
     primer_bind     188..209
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    285..318
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(414..437)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 tcttataagt tctgtatgag accacagatc tagttggaac cattcaaaac agcatagcaa
       61 gttaaaataa ggctagtccg ttatcaactt gaaaaagtgg caccgagtcg gtgctttttg
      121 gcgccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc
      181 gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg
      241 agctgtacaa gtaagaattc cgatcatatt caataaccct taatataact tcgtataatg
      301 tatgctatac gaagttatta ggtctgaaga ggagtttacg tccagccaag cttaggatct
      361 cgacctcgaa attctaccgg gtaggggagg cgcttttccc aaggcagtct ggagcatgcg
      421 ctttagcagc cccgctgggc acttggcgct acacaagtgg cctctggcct cgcacacatt
      481 ccacatccac cggtaggcgc caaccggctc cgttctttgg tggccccttc gcgccacctt
      541 ctactcctcc cctagtcagg aagttccccc ccgccccgca gctcgcgtcg tgcaggacgt
      601 gacaaatgga agtagcacgt ctcactagtc tcgtgcagat ggacagcacc gctgagcaat
      661 ggaagcgggt aggcctttgg ggcagcggcc aatagca
//
