LOCUS       sequence-137789-         744 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To express in mammalian cells  human PolQ.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 744)
  AUTHORS   Mateos-Gomez PA, Gong F, Nair N, Miller KM, Lazzerini-Denchi E, 
            Sfeir A
  TITLE     Mammalian polymerase theta promotes alternative NHEJ and suppresses 
            recombination.
  JOURNAL   Nature. 2015 Feb 12;518(7538):254-7. doi: 10.1038/nature14157. Epub 
            2015 Feb 2.
  PUBMED    25642960
REFERENCE   2  (bases 1 to 744)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature14157"; journalName: "Nature"; date: "2015-02-12- 
            12"; volume: "518"; issue: "7538"; pages: "254-7"
COMMENT     Sequence Label: pcDNA 3.1(Hygro) myc-hPolQ-Flag
FEATURES             Location/Qualifiers
     source          1..744
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(15..38)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gactcgagct attacttgtc gtcatcgtct ttgtagtcca tcccgggcac atcaaagtcc
       61 tttagctctc cccagctggc gcctattttc actttcactt tcaatttcac agacagtttt
      121 acagcacttt ccatttcatt cttgacaatc tgagctacct gaacaacatc ttcttctgcc
      181 acttcatata ggagttcatc atggagttga aggatgaaga agcctcctct gattgggcag
      241 aacatccctt gcagttttct ctttcgtgac aatcctgttc ggtcactttg gagcataccc
      301 tctcgatgac catgggattt gaaggttgag tggaaggtct ctaattgctt ctgaatgtta
      361 actgtggcta ttttgacaat atcagctgct gatccttgga ctattgtgtt gatagcttga
      421 cgctcagcgt gagctttacg ataagggttg ttgtctttga ttcctggcaa atatctacgc
      481 cttcccaaaa tggtctgaac aaatccgtct cttttacaat tcttcactgt ctctgtcatg
      541 aattgattaa tccctgtgta tctggatttg aaggagtcaa tatagcatgc agcatcattt
      601 tctttaatgc ccatctgctc tcccaaagat ttagctccca ttccataaat gatcccatag
      661 caaatctgtt ttgcctgctg cctcagatca tccccaacag actctggctc aatcatcttc
      721 cactctgctg caatgctcct gaaa
//
