LOCUS       sequence-137794-         614 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of chick Cx45.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 614)
  AUTHORS   Veenstra RD, Wang HZ, Westphale EM, Beyer EC
  TITLE     Multiple connexins confer distinct regulatory and conductance 
            properties of gap junctions in developing heart.
  JOURNAL   Circ Res. 1992 Nov;71(5):1277-83.
  PUBMED    1382884
REFERENCE   2  (bases 1 to 614)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Circ Res.";
            date: "1992-11"; volume: "71(5)"; pages: "1277-83"
COMMENT     Sequence Label: pSFFV-Cx45
FEATURES             Location/Qualifiers
     source          1..614
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(35..54)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(36..54)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(61..78)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(61..77)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(70..92)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      219..599
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(306..325)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     516..537
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 agagcggccg ccaccgcggt ggagctccaa ttcgccctat agtgagtcgt attacaattc
       61 actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg
      121 ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg
      181 cccttcccaa cagttgcgca gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt
      241 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
      301 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
      361 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
      421 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
      481 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
      541 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
      601 ctattggtta aaaa
//
