LOCUS       sequence-137811-         739 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For mammalian expression of an N-terminally triple FLAG-tagged and 
            C-terminally triple AU1-tagged full length human GATA6 with triple 
            alanine mutations on aminoacids#33,#34 and #37.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 739)
  AUTHORS   Chitforoushzadeh Z, Ye Z, Sheng Z, LaRue S, Fry RC, Lauffenburger 
            DA, Janes KA
  TITLE     TNF-insulin crosstalk at the transcription factor GATA6 is revealed 
            by a model that links signaling and transcriptomic data tensors.
  JOURNAL   Sci Signal. 2016 Jun 7;9(431):ra59. doi: 10.1126/scisignal.aad3373.
  PUBMED    27273097
REFERENCE   2  (bases 1 to 739)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2016-06-7"; volume: "9(431):ra59. doi"; pages: " 
            10.1126/scisignal.aad3373"
COMMENT     Sequence Label: pBabe 3XFLAG-S/T3A-GATA6-3XAU1 puro
FEATURES             Location/Qualifiers
     source          1..739
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             10..81
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags"
                     /label=3xFLAG
                     /translation="DYKDDDDKDYKDDDDKDYKDDDDK"
ORIGIN
        1 ggatccatgg actacaagga tgacgatgac aaggactaca aggatgacga tgacaaggac
       61 tacaaggatg acgatgacaa gatggccttg actgacggcg gctggtgctt gccgaagcgc
      121 ttcggggccg cgggtgcgga cgccagcgac tccagagcct ttccagcgcg ggagcccgcc
      181 gcgccgcctg cccccatctc ttcctcgtcc tcctcctgct cccggggcgg agagcggggc
      241 cccggcggcg ccagcaactg cgggacgcct cagctcgaca cggaggcggc ggccggaccc
      301 ccggcccgct cgctgctgct cagttcctac gcttcgcatc ccttcggggc tccccacgga
      361 ccttcggcgc ctggggtcgc gggccccggg ggcaacctgt cgagctggga ggacttgctg
      421 ctgttcactg acctcgacca agccgcgacc gccagcaagc tgctgtggtc cagccgcggc
      481 gccaagctga gccccttcgc acccgagcag ccggaggaga tgtaccagac cctcgccgct
      541 ctctccagcc agggtccggc cgcctacgac ggcgcgcccg gcggcttcgt gcactctgcg
      601 gccgcggcgg cagcagccgc ggcggcggcc agctccccgg tctacgtgcc caccacccgc
      661 gtgggttcca tgctgcccgg cctaccgtac cacctgcagg ggtcgggcag tgggccagcc
      721 aaccacgcgg gcggcgcgg
//
