LOCUS       sequence-137826-       15841 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15841)
  AUTHORS   Osakada F, Mori T, Cetin AH, Marshel JH, Virgen B, Callaway EM
  TITLE     New rabies virus variants for monitoring and manipulating activity 
            and gene expression in defined neural circuits.
  JOURNAL   Neuron. 2011 Aug 25;71(4):617-31.
  PUBMED    21867879
REFERENCE   2  (bases 1 to 15841)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron."; 
            date: "2011-08-25"; volume: "71(4)"; pages: "617-31"
COMMENT     Sequence Label: pSADdeltaG-GFP-F2
FEATURES             Location/Qualifiers
     source          1..15841
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     629..648
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      4043..4052
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4049..4051,4052..4054,4055..4768)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPNNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4094..4115)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4355..4374)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4702..4723
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             6506..6517
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(11615..11632)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    11621..11845
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      11891..12319
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11978..11997)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     12188..12209
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(12328..12348)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        12333..12662
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      12513..12648
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     12575..12594
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        12710..12757
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             12776..13150
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    13280..13401
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(13317..13336)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     13371..13390
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(13450..13466)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13450..13466)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(13463..13485)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    13474..13490
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(13498..13504,13505..13522,13523..13528))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13543..13564
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(13681..13698)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(13852..14440)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13932..13951)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(14611..15402,15403..15471))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     15234..15253
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(15472..15576)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(15651..15670)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctg
      661 gctagattaa gcgtctgatg agtccgtgag gacgaaaccc ggagtcccgg gtcacgctta
      721 acaaccagat caaagaaaaa acagacattg tcaattgcaa agcaaaaatg taacacccct
      781 acaatggatg ccgacaagat tgtattcaaa gtcaataatc aggtggtctc tttgaagcct
      841 gagattatcg tggatcaata tgagtacaag taccctgcca tcaaagattt gaaaaagccc
      901 tgtataaccc taggaaaggc tcccgattta aataaagcat acaagtcagt tttgtcaggc
      961 atgagcgccg ccaaacttaa tcctgacgat gtatgttcct atttggcagc ggcaatgcag
     1021 ttttttgagg ggacatgtcc ggaagactgg accagctatg gaattgtgat tgcacgaaaa
     1081 ggagataaga tcaccccagg ttctctggtg gagataaaac gtactgatgt agaagggaat
     1141 tgggctctga caggaggcat ggaactgaca agagacccca ctgtccctga gcatgcgtcc
     1201 ttagtcggtc ttctcttgag tctgtatagg ttgagcaaaa tatccgggca aaacactggt
     1261 aactataaga caaacattgc agacaggata gagcagattt ttgagacagc cccttttgtt
     1321 aaaatcgtgg aacaccatac tctaatgaca actcacaaaa tgtgtgctaa ttggagtact
     1381 ataccaaact tcagattttt ggccggaacc tatgacatgt ttttctcccg gattgagcat
     1441 ctatattcag caatcagagt gggcacagtt gtcactgctt atgaagactg ttcaggactg
     1501 gtatcattta ctgggttcat aaaacaaatc aatctcaccg ctagagaggc aatactatat
     1561 ttcttccaca agaactttga ggaagagata agaagaatgt ttgagccagg gcaggagaca
     1621 gctgttcctc actcttattt catccacttc cgttcactag gcttgagtgg gaaatctcct
     1681 tattcatcaa atgctgttgg tcacgtgttc aatctcattc actttgtagg atgctatatg
     1741 ggtcaagtca gatccctaaa tgcaacggtt attgctgcat gtgctcctca tgaaatgtct
     1801 gttctagggg gctatctggg agaggaattc ttcgggaaag ggacatttga aagaagattc
     1861 ttcagagatg agaaagaact tcaagaatac gaggcggctg aactgacaaa gactgacgta
     1921 gcactggcag atgatggaac tgtcaactct gacgacgagg actacttttc aggtgaaacc
     1981 agaagtccgg aggctgttta tactcgaatc atgatgaatg gaggtcgact aaagagatct
     2041 cacatacgga gatatgtctc agtcagttcc aatcatcaag cccgtccaaa ctcattcgcc
     2101 gagtttctaa acaagacata ttcgagtgac tcataagaag ttgaataaca aaatgccgga
     2161 aatctacgga ttgtgtatat ccatcatgaa aaaaactaac acccctcctt tcgaaccatc
     2221 ccaaacatga gcaagatctt tgtcaatcct agtgctatta gagccggtct ggccgatctt
     2281 gagatggctg aagaaactgt tgatctgatc aatagaaata tcgaagacaa tcaggctcat
     2341 ctccaagggg aacccataga ggtggacaat ctccctgagg atatggggcg acttcacctg
     2401 gatgatggaa aatcgcccaa ccatggtgag atagccaagg tgggagaagg caagtatcga
     2461 gaggactttc agatggatga aggagaggat cctagcttcc tgttccagtc atacctggaa
     2521 aatgttggag tccaaatagt cagacaaatg aggtcaggag agagatttct caagatatgg
     2581 tcacagaccg tagaagagat tatatcctat gtcgcggtca actttcccaa ccctccagga
     2641 aagtcttcag aggataaatc aacccagact actggccgag agctcaagaa ggagacaaca
     2701 cccactcctt ctcagagaga aagccaatca tcgaaagcca ggatggcggc tcaaattgct
     2761 tctggccctc cagcccttga atggtcggct accaatgaag aggatgatct atcagtggag
     2821 gctgagatcg ctcaccagat tgcagaaagt ttctccaaaa aatataagtt tccctctcga
     2881 tcctcaggga tactcttgta taattttgag caattgaaaa tgaaccttga tgatatagtt
     2941 aaagaggcaa aaaatgtacc aggtgtgacc cgtttagccc atgacgggtc caaactcccc
     3001 ctaagatgtg tactgggatg ggtcgctttg gccaactcta agaaattcca gttgttagtc
     3061 gaatccgaca agctgagtaa aatcatgcaa gatgacttga atcgctatac atcttgctaa
     3121 ccgaacctct cccctcagtc cctctagaca ataaaatccg agatgtccca aagtcaacat
     3181 gaaaaaaaca ggcaacacca ctgataaatc gatgaacctc ctacgtaaga tagtgaaaaa
     3241 ccgcagggac gaggacactc aaaaatcctc tcccgcgtca gcccctctgg atgacgatga
     3301 cttgtggctt ccaccccctg aatacgtccc gctgaaagaa cttacaggca agaagaacat
     3361 gaggaacttt tgtatcaacg gaagggttaa agtgtgtagc ccgaatggtt actcgttcag
     3421 gatcctgcgg cacattctga aatcattcga cgagatatat tctgggaatc ataggatgat
     3481 cgggttagtc aaagtggtta ttggactggc tttgtcagga tctccagtcc ctgagggcct
     3541 gaactgggta tacaaattga ggagaacctt tatcttccag tgggctgatt ccaggggccc
     3601 tcttgaaggg gaggagttgg aatactctca ggagatcact tgggatgatg atactgagtt
     3661 cgtcggattg caaataagag tgattgcaaa acagtgtcat atccagggca gagtctggtg
     3721 tatcaacatg aacccgagag catgtcaact atggtctgac atgtctcttc agacacaaag
     3781 gtccgaagag gacaaagatt cctctctgct tctagaataa cgtacgtcag attatatccc
     3841 gcaaatttat cacttgttta cctctggagg agagaacata tgggctcaac tccaaccctt
     3901 gggagcaata taacaaaaaa catgttatgg tgccattaaa ccgctgcatt tcatcaaagt
     3961 caagttgatt acctttacat tttgatcctc ttggatgtga aaaaaactat taacatccct
     4021 caaaggacct gcaggatcca tcgccaccat ggtgagcaag ggcgaggagc tgttcaccgg
     4081 ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc
     4141 cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac
     4201 cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg
     4261 cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga
     4321 aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc
     4381 cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt
     4441 caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt
     4501 ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa
     4561 catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga
     4621 cggccccgtg ctgctgccca acaaccacta cctgagcacc cagtccgccc tgagcaaaga
     4681 ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac
     4741 tctcggcatg gacgagctgt acaagtaaag cggccgaccc gcggtagctt ttcagtcgag
     4801 aaaaaaacat tagatcagaa gaacaactgg caacacttct caacctgaga cttacttcaa
     4861 gatgctcgat cctggagagg tctatgatga ccctattgac ccaatcgagt tagaggctga
     4921 acccagagga acccccattg tccccaacat cttgaggaac tctgactaca atctcaactc
     4981 tcctttgata gaagatcctg ctagactaat gttagaatgg ttaaaaacag ggaatagacc
     5041 ttatcggatg actctaacag acaattgctc caggtctttc agagttttga aagattattt
     5101 caagaaggta gatttgggtt ctctcaaggt gggcggaatg gctgcacagt caatgatttc
     5161 tctctggtta tatggtgccc actctgaatc caacaggagc cggagatgta taacagactt
     5221 ggcccatttc tattccaagt cgtcccccat agagaagctg ttgaatctca cgctaggaaa
     5281 tagagggctg agaatccccc cagagggagt gttaagttgc cttgagaggg ttgattatga
     5341 taatgcattt ggaaggtatc ttgccaacac gtattcctct tacttgttct tccatgtaat
     5401 caccttatac atgaacgccc tagactggga tgaagaaaag accatcctag cattatggaa
     5461 agatttaacc tcagtggaca tcgggaagga cttggtaaag ttcaaagacc aaatatgggg
     5521 actgctgatc gtgacaaagg actttgttta ctcccaaagt tccaattgtc tttttgacag
     5581 aaactacaca cttatgctaa aagatctttt cttgtctcgc ttcaactcct taatggtctt
     5641 gctctctccc ccagagcccc gatactcaga tgacttgata tctcaactat gccagctgta
     5701 cattgctggg gatcaagtct tgtctatgtg tggaaactcc ggctatgaag tcatcaaaat
     5761 attggagcca tatgtcgtga atagtttagt ccagagagca gaaaagttta ggcctctcat
     5821 tcattccttg ggagactttc ctgtatttat aaaagacaag gtaagtcaac ttgaagagac
     5881 gttcggtccc tgtgcaagaa ggttctttag ggctctggat caattcgaca acatacatga
     5941 cttggttttt gtgtttggct gttacaggca ttgggggcac ccatatatag attatcgaaa
     6001 gggtctgtca aaactatatg atcaggttca ccttaaaaaa atgatagata agtcctacca
     6061 ggagtgctta gcaagcgacc tagccaggag gatccttaga tggggttttg ataagtactc
     6121 caagtggtat ctggattcaa gattcctagc ccgagaccac cccttgactc cttatatcaa
     6181 aacccaaaca tggccaccca aacatattgt agacttggtg ggggatacat ggcacaagct
     6241 cccgatcacg cagatctttg agattcctga atcaatggat ccgtcagaaa tattggatga
     6301 caaatcacat tctttcacca gaacgagact agcttcttgg ctgtcagaaa accgaggggg
     6361 gcctgttcct agcgaaaaag ttattatcac ggccctgtct aagccgcctg tcaatccccg
     6421 agagtttctg aggtctatag acctcggagg attgccagat gaagacttga taattggcct
     6481 caagccaaag gaacgggaat tgaagattga aggtcgattc tttgctctaa tgtcatggaa
     6541 tctaagattg tattttgtca tcactgaaaa actcttggcc aactacatct tgccactttt
     6601 tgacgcgctg actatgacag acaacctgaa caaggtgttt aaaaagctga tcgacagggt
     6661 caccgggcaa gggcttttgg actattcaag ggtcacatat gcatttcacc tggactatga
     6721 aaagtggaac aaccatcaaa gattagagtc aacagaggat gtattttctg tcctagatca
     6781 agtgtttgga ttgaagagag tgttttctag aacacacgag ttttttcaaa aggcctggat
     6841 ctattattca gacagatcag acctcatcgg gttacgggag gatcaaatat actgcttaga
     6901 tgcgtccaac ggcccaacct gttggaatgg ccaggatggc gggctagaag gcttacggca
     6961 gaagggctgg agtctagtca gcttattgat gatagataga gaatctcaaa tcaggaacac
     7021 aagaaccaaa atactagctc aaggagacaa ccaggtttta tgtccgacat acatgttgtc
     7081 gccagggcta tctcaagagg ggctcctcta tgaattggag agaatatcaa ggaatgcact
     7141 ttcgatatac agagccgtcg aggaaggggc atctaagcta gggctgatca tcaagaaaga
     7201 agagaccatg tgtagttatg acttcctcat ctatggaaaa acccctttgt ttagaggtaa
     7261 catattggtg cctgagtcca aaagatgggc cagagtctct tgcgtctcta atgaccaaat
     7321 agtcaacctc gccaatataa tgtcgacagt gtccaccaat gcgctaacag tggcacaaca
     7381 ctctcaatct ttgatcaaac cgatgaggga ttttctgctc atgtcagtac aggcagtctt
     7441 tcactacctg ctatttagcc caatcttaaa gggaagagtt tacaagattc tgagcgctga
     7501 aggggagagc tttctcctag ccatgtcaag gataatctat ctagatcctt ctttgggagg
     7561 gatatctgga atgtccctcg gaagattcca tatacgacag ttctcagacc ctgtctctga
     7621 agggttatcc ttctggagag agatctggtt aagctcccaa gagtcctgga ttcacgcgtt
     7681 gtgtcaagag gctggaaacc cagatcttgg agagagaaca ctcgagagct tcactcgcct
     7741 tctagaagat ccgaccacct taaatatcag aggaggggcc agtcctacca ttctactcaa
     7801 ggatgcaatc agaaaggctt tatatgacga ggtggacaag gtggaaaatt cagagtttcg
     7861 agaggcaatc ctgttgtcca agacccatag agataatttt atactcttct taatatctgt
     7921 tgagcctctg tttcctcgat ttctcagtga gctattcagt tcgtcttttt tgggaatccc
     7981 cgagtcaatc attggattga tacaaaactc ccgaacgata agaaggcagt ttagaaagag
     8041 tctctcaaaa actttagaag aatccttcta caactcagag atccacggga ttagtcggat
     8101 gacccagaca cctcagaggg ttgggggggt gtggccttgc tcttcagaga gggcagatct
     8161 acttagggag atctcttggg gaagaaaagt ggtaggcacg acagttcctc acccttctga
     8221 gatgttggga ttacttccca agtcctctat ttcttgcact tgtggagcaa caggaggagg
     8281 caatcctaga gtttctgtat cagtactccc gtcctttgat cagtcatttt tttcacgagg
     8341 ccccctaaag ggatacttgg gctcgtccac ctctatgtcg acccagctat tccatgcatg
     8401 ggaaaaagtc actaatgttc atgtggtgaa gagagctcta tcgttaaaag aatctataaa
     8461 ctggttcatt actagagatt ccaacttggc tcaagctcta attaggaaca ttatgtctct
     8521 gacaggccct gatttccctc tagaggaggc ccctgtcttc aaaaggacgg ggtcagcctt
     8581 gcataggttc aagtctgcca gatacagcga aggagggtat tcttctgtct gcccgaacct
     8641 cctctctcat atttctgtta gtacagacac catgtctgat ttgacccaag acgggaagaa
     8701 ctacgatttc atgttccagc cattgatgct ttatgcacag acatggacat cagagctggt
     8761 acagagagac acaaggctaa gagactctac gtttcattgg cacctccgat gcaacaggtg
     8821 tgtgagaccc attgacgacg tgaccctgga gacctctcag atcttcgagt ttccggatgt
     8881 gtcgaaaaga atatccagaa tggtttctgg ggctgtgcct cacttccaga ggcttcccga
     8941 tatccgtctg agaccaggag attttgaatc tctaagcggt agagaaaagt ctcaccatat
     9001 cggatcagct caggggctct tatactcaat cttagtggca attcacgact caggatacaa
     9061 tgatggaacc atcttccctg tcaacatata cggcaaggtt tcccctagag actatttgag
     9121 agggctcgca aggggagtat tgataggatc ctcgatttgc ttcttgacaa gaatgacaaa
     9181 tatcaatatt aatagacctc ttgaattggt ctcaggggta atctcatata ttctcctgag
     9241 gctagataac catccctcct tgtacataat gctcagagaa ccgtctctta gaggagagat
     9301 attttctatc cctcagaaaa tccccgccgc ttatccaacc actatgaaag aaggcaacag
     9361 atcaatcttg tgttatctcc aacatgtgct acgctatgag cgagagataa tcacggcgtc
     9421 tccagagaat gactggctat ggatcttttc agactttaga agtgccaaaa tgacgtacct
     9481 atccctcatt acttaccagt ctcatcttct actccagagg gttgagagaa acctatctaa
     9541 gagtatgaga gataacctgc gacaattgag ttctttgatg aggcaggtgc tgggcgggca
     9601 cggagaagat accttagagt cagacgacaa cattcaacga ctgctaaaag actctttacg
     9661 aaggacaaga tgggtggatc aagaggtgcg ccatgcagct agaaccatga ctggagatta
     9721 cagccccaac aagaaggtgt cccgtaaggt aggatgttca gaatgggtct gctctgctca
     9781 acaggttgca gtctctacct cagcaaaccc ggcccctgtc tcggagcttg acataagggc
     9841 cctctctaag aggttccaga accctttgat ctcgggcttg agagtggttc agtgggcaac
     9901 cggtgctcat tataagctta agcctattct agatgatctc aatgttttcc catctctctg
     9961 ccttgtagtt ggggacgggt caggggggat atcaagggca gtcctcaaca tgtttccaga
    10021 tgccaagctt gtgttcaaca gtcttttaga ggtgaatgac ctgatggctt ccggaacaca
    10081 tccactgcct ccttcagcaa tcatgagggg aggaaatgat atcgtctcca gagtgataga
    10141 tcttgactca atctgggaaa aaccgtccga cttgagaaac ttggcaacct ggaaatactt
    10201 ccagtcagtc caaaagcagg tcaacatgtc ctatgacctc attatttgcg atgcagaagt
    10261 tactgacatt gcatctatca accggatcac cctgttaatg tccgattttg cattgtctat
    10321 agatggacca ctctatttgg tcttcaaaac ttatgggact atgctagtaa atccaaacta
    10381 caaggctatt caacacctgt caagagcgtt cccctcggtc acagggttta tcacccaagt
    10441 aacttcgtct ttttcatctg agctctacct ccgattctcc aaacgaggga agtttttcag
    10501 agatgctgag tacttgacct cttccaccct tcgagaaatg agccttgtgt tattcaattg
    10561 tagcagcccc aagagtgaga tgcagagagc tcgttccttg aactatcagg atcttgtgag
    10621 aggatttcct gaagaaatca tatcaaatcc ttacaatgag atgatcataa ctctgattga
    10681 cagtgatgta gaatcttttc tagtccacaa gatggttgat gatcttgagt tacagagggg
    10741 aactctgtct aaagtggcta tcattatagc catcatgata gttttctcca acagagtctt
    10801 caacgtttcc aaacccctaa ctgacccctc gttctatcca ccgtctgatc ccaaaatcct
    10861 gaggcacttc aacatatgtt gcagtactat gatgtatcta tctactgctt taggtgacgt
    10921 ccctagcttc gcaagacttc acgacctgta taacagacct ataacttatt acttcagaaa
    10981 gcaagtcatt cgagggaacg tttatctatc ttggagttgg tccaacgaca cctcagtgtt
    11041 caaaagggta gcctgtaatt ctagcctgag tctgtcatct cactggatca ggttgattta
    11101 caagatagtg aagactacca gactcgttgg cagcatcaag gatctatcca gagaagtgga
    11161 aagacacctt cataggtaca acaggtggat caccctagag gatatcagat ctagatcatc
    11221 cctactagac tacagttgcc tgtgaaccgg atactcctgg aagcctgccc atgctaagac
    11281 tcttgtgtga tgtatcttga aaaaaacaag atcctaaatc tgaacctttg gttgtttgat
    11341 tgtttttctc atttttgttg tttatttgtt aagcgtgggt cggcatggca tctccacctc
    11401 ctcgcggtcc gacctgggca tccgaaggag gacgcacgtc cactcggatg gctaagggag
    11461 aagggcgaat tccagcacac tggcggccgt tactagtgga tccgagctcg gtaccgagct
    11521 cggatccact agtccagtgt ggtggaattc tgcagatatc cagcacagtg gcggccgctc
    11581 gagtctagag ggcccgttta aacccgctga tcagcctcga ctgtgccttc tagttgccag
    11641 ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact
    11701 gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt
    11761 ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat
    11821 gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg
    11881 gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
    11941 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
    12001 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
    12061 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
    12121 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
    12181 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
    12241 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
    12301 caaaaattta acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc
    12361 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt
    12421 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
    12481 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
    12541 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
    12601 ctgcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca
    12661 aaaagctccc gggagcttgt atatccattt tcggatctga tcagcacgtg ttgacaatta
    12721 atcatcggca tagtatatcg gcatagtata atacgacaag gtgaggaact aaaccatggc
    12781 caagttgacc agtgccgttc cggtgctcac cgcgcgcgac gtcgccggag cggtcgagtt
    12841 ctggaccgac cggctcgggt tctcccggga cttcgtggag gacgacttcg ccggtgtggt
    12901 ccgggacgac gtgaccctgt tcatcagcgc ggtccaggac caggtggtgc cggacaacac
    12961 cctggcctgg gtgtgggtgc gcggcctgga cgagctgtac gccgagtggt cggaggtcgt
    13021 gtccacgaac ttccgggacg cctccgggcc ggccatgacc gagatcggcg agcagccgtg
    13081 ggggcgggag ttcgccctgc gcgacccggc cggcaactgc gtgcacttcg tggccgagga
    13141 gcaggactga cacgtgctac gagatttcga ttccaccgcc gccttctatg aaaggttggg
    13201 cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct
    13261 ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa
    13321 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
    13381 caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc
    13441 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
    13501 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
    13561 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
    13621 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
    13681 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
    13741 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
    13801 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
    13861 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
    13921 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
    13981 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
    14041 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
    14101 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
    14161 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
    14221 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
    14281 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
    14341 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
    14401 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
    14461 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
    14521 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
    14581 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
    14641 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
    14701 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
    14761 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
    14821 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
    14881 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
    14941 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
    15001 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
    15061 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
    15121 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
    15181 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
    15241 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
    15301 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
    15361 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
    15421 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
    15481 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
    15541 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
    15601 tgacgtcgac ggatcgggag atctcccgat cccctatggt gcactctcag tacaatctgc
    15661 tctgatgccg catagttaag ccagtatctg ctccctgctt gtgtgttgga ggtcgctgag
    15721 tagtgcgcga gcaaaattta agctacaaca aggcaaggct tgaccgacaa ttgcatgaag
    15781 aatctgctta gggttaggcg ttttgcgctg cttcgcgatg tacgggccag atatacgcgt
    15841 t
//
