LOCUS       sequence-137827-       16208 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16208)
  AUTHORS   Osakada F, Mori T, Cetin AH, Marshel JH, Virgen B, Callaway EM
  TITLE     New rabies virus variants for monitoring and manipulating activity 
            and gene expression in defined neural circuits.
  JOURNAL   Neuron. 2011 Aug 25;71(4):617-31.
  PUBMED    21867879
REFERENCE   2  (bases 1 to 16208)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron."; 
            date: "2011-08-25"; volume: "71(4)"; pages: "617-31"
COMMENT     Sequence Label: pSADdeltaG-mCherry
FEATURES             Location/Qualifiers
     source          1..16208
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     629..648
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             4095..4805
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(4240..4258)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4508..4527
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             6529..6540
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(11565..11582)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    11571..11795
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      11841..12269
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11928..11947)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     12138..12159
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(12278..12298)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        12283..12612
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      12463..12598
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     12525..12544
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             12679..13473
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(12733..12752)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     13343..13362
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    13647..13768
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(13684..13703)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     13738..13757
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(13817..13833)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13817..13833)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(13830..13852)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    13841..13857
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(13865..13871,13872..13889,13890..13895))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13910..13931
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(14048..14065)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(14219..14807)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(14299..14318)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(14978..15769,15770..15838))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     15601..15620
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(15839..15943)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(16018..16037)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctg
      661 gctagcgttt aaacgggccc tctagactcg agcggccgcc agtgtgatgg atatctgcag
      721 aattcgccct tttaagcgtc tgatgagtcc gtgaggacga aacccggagt cccgggtcac
      781 gcttaacaac cagatcaaag aaaaaacaga cattgtcaat tgcaaagcaa aaatgtaaca
      841 cccctacaat ggatgccgac aagattgtat tcaaagtcaa taatcaggtg gtctctttga
      901 agcctgagat tatcgtggat caatatgagt acaagtaccc tgccatcaaa gatttgaaaa
      961 agccctgtat aaccctagga aaggctcccg atttaaataa agcatacaag tcagttttgt
     1021 caggcatgag cgccgccaaa cttaatcctg acgatgtatg ttcctatttg gcagcggcaa
     1081 tgcagttttt tgaggggaca tgtccggaag actggaccag ctatggaatt gtgattgcac
     1141 gaaaaggaga taagatcacc ccaggttctc tggtggagat aaaacgtact gatgtagaag
     1201 ggaattgggc tctgacagga ggcatggaac tgacaagaga ccccactgtc cctgagcatg
     1261 cgtccttagt cggtcttctc ttgagtctgt ataggttgag caaaatatcc gggcaaaaca
     1321 ctggtaacta taagacaaac attgcagaca ggatagagca gatttttgag acagcccctt
     1381 ttgttaaaat cgtggaacac catactctaa tgacaactca caaaatgtgt gctaattgga
     1441 gtactatacc aaacttcaga tttttggccg gaacctatga catgtttttc tcccggattg
     1501 agcatctata ttcagcaatc agagtgggca cagttgtcac tgcttatgaa gactgttcag
     1561 gactggtatc atttactggg ttcataaaac aaatcaatct caccgctaga gaggcaatac
     1621 tatatttctt ccacaagaac tttgaggaag agataagaag aatgtttgag ccagggcagg
     1681 agacagctgt tcctcactct tatttcatcc acttccgttc actaggcttg agtgggaaat
     1741 ctccttattc atcaaatgct gttggtcacg tgttcaatct cattcacttt gtaggatgct
     1801 atatgggtca agtcagatcc ctaaatgcaa cggttattgc tgcatgtgct cctcatgaaa
     1861 tgtctgttct agggggctat ctgggagagg aattcttcgg gaaagggaca tttgaaagaa
     1921 gattcttcag agatgagaaa gaacttcaag aatacgaggc ggctgaactg acaaagactg
     1981 acgtagcact ggcagatgat ggaactgtca actctgacga cgaggactac ttttcaggtg
     2041 aaaccagaag tccggaggct gtttatactc gaatcatgat gaatggaggt cgactaaaga
     2101 gatctcacat acggagatat gtctcagtca gttccaatca tcaagcccgt ccaaactcat
     2161 tcgccgagtt tctaaacaag acatattcga gtgactcata agaagttgaa taacaaaatg
     2221 ccggaaatct acggattgtg tatatccatc atgaaaaaaa ctaacacccc tcctttcgaa
     2281 ccatcccaaa catgagcaag atctttgtca atcctagtgc tattagagcc ggtctggccg
     2341 atcttgagat ggctgaagaa actgttgatc tgatcaatag aaatatcgaa gacaatcagg
     2401 ctcatctcca aggggaaccc atagaggtgg acaatctccc tgaggatatg gggcgacttc
     2461 acctggatga tggaaaatcg cccaaccatg gtgagatagc caaggtggga gaaggcaagt
     2521 atcgagagga ctttcagatg gatgaaggag aggatcctag cttcctgttc cagtcatacc
     2581 tggaaaatgt tggagtccaa atagtcagac aaatgaggtc aggagagaga tttctcaaga
     2641 tatggtcaca gaccgtagaa gagattatat cctatgtcgc ggtcaacttt cccaaccctc
     2701 caggaaagtc ttcagaggat aaatcaaccc agactactgg ccgagagctc aagaaggaga
     2761 caacacccac tccttctcag agagaaagcc aatcatcgaa agccaggatg gcggctcaaa
     2821 ttgcttctgg ccctccagcc cttgaatggt cggctaccaa tgaagaggat gatctatcag
     2881 tggaggctga gatcgctcac cagattgcag aaagtttctc caaaaaatat aagtttccct
     2941 ctcgatcctc agggatactc ttgtataatt ttgagcaatt gaaaatgaac cttgatgata
     3001 tagttaaaga ggcaaaaaat gtaccaggtg tgacccgttt agcccatgac gggtccaaac
     3061 tccccctaag atgtgtactg ggatgggtcg ctttggccaa ctctaagaaa ttccagttgt
     3121 tagtcgaatc cgacaagctg agtaaaatca tgcaagatga cttgaatcgc tatacatctt
     3181 gctaaccgaa cctctcccct cagtccctct agacaataaa atccgagatg tcccaaagtc
     3241 aacatgaaaa aaacaggcaa caccactgat aaaatgaacc tcctacgtaa gatagtgaaa
     3301 aaccgcaggg acgaggacac tcaaaaatcc tctcccgcgt cagcccctct ggatgacgat
     3361 gacttgtggc ttccaccccc tgaatacgtc ccgctgaaag aacttacagg caagaagaac
     3421 atgaggaact tttgtatcaa cggaagggtt aaagtgtgta gcccgaatgg ttactcgttc
     3481 aggatcctgc ggcacattct gaaatcattc gacgagatat attctgggaa tcataggatg
     3541 atcgggttag tcaaagtggt tattggactg gctttgtcag gatctccagt ccctgagggc
     3601 ctgaactggg tatacaaatt gaggagaacc tttatcttcc agtgggctga ttccaggggc
     3661 cctcttgaag gggaggagtt ggaatactct caggagatca cttgggatga tgatactgag
     3721 ttcgtcggat tgcaaataag agtgattgca aaacagtgtc atatccaggg cagagtctgg
     3781 tgtatcaaca tgaacccgag agcatgtcaa ctatggtctg acatgtctct tcagacacaa
     3841 aggtccgaag aggacaaaga ttcctctctg cttctagaat aatcagatta tatcccgcaa
     3901 atttatcact tgtttacctc tggaggagag aacatatggg ctcaactcca acccttggga
     3961 gcaatataac aaaaaacatg ttatggtgcc attaaaccgc tgcatttcat caaagtcaag
     4021 ttgattacct ttacattttg atcctcttgg atgtgaaaaa aactattaac atccctcaaa
     4081 ggacgggatc caccatggtg agcaagggcg aggaggataa catggccatc atcaaggagt
     4141 tcatgcgctt caaggtgcac atggagggct ccgtgaacgg ccacgagttc gagatcgagg
     4201 gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag gtgaccaagg
     4261 gtggccccct gcccttcgcc tgggacatcc tgtcccctca gttcatgtac ggctccaagg
     4321 cctacgtgaa gcaccccgcc gacatccccg actacttgaa gctgtccttc cccgagggct
     4381 tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg acccaggact
     4441 cctccctgca ggacggcgag ttcatctaca aggtgaagct gcgcggcacc aacttcccct
     4501 ccgacggccc cgtaatgcag aagaagacca tgggctggga ggcctcctcc gagcggatgt
     4561 accccgagga cggcgccctg aagggcgaga tcaagcagag gctgaagctg aaggacggcg
     4621 gccactacga cgctgaggtc aagaccacct acaaggccaa gaagcccgtg cagctgcccg
     4681 gcgcctacaa cgtcaacatc aagttggaca tcacctccca caacgaggac tacaccatcg
     4741 tggaacagta cgaacgcgcc gagggccgcc actccaccgg cggcatggac gagctgtaca
     4801 agtaagctag cttttcagtc gagaaaaaaa cattagatca gaagaacaac tggcaacact
     4861 tctcaacctg agacttactt caagatgctc gatcctggag aggtctatga tgaccctatt
     4921 gacccaatcg agttagaggc tgaacccaga ggaaccccca ttgtccccaa catcttgagg
     4981 aactctgact acaatctcaa ctctcctttg atagaagatc ctgctagact aatgttagaa
     5041 tggttaaaaa cagggaatag accttatcgg atgactctaa cagacaattg ctccaggtct
     5101 ttcagagttt tgaaagatta tttcaagaag gtagatttgg gttctctcaa ggtgggcgga
     5161 atggctgcac agtcaatgat ttctctctgg ttatatggtg cccactctga atccaacagg
     5221 agccggagat gtataacaga cttggcccat ttctattcca agtcgtcccc catagagaag
     5281 ctgttgaatc tcacgctagg aaatagaggg ctgagaatcc ccccagaggg agtgttaagt
     5341 tgccttgaga gggttgatta tgataatgca tttggaaggt atcttgccaa cacgtattcc
     5401 tcttacttgt tcttccatgt aatcacctta tacatgaacg ccctagactg ggatgaagaa
     5461 aagaccatcc tagcattatg gaaagattta acctcagtgg acatcgggaa ggacttggta
     5521 aagttcaaag accaaatatg gggactgctg atcgtgacaa aggactttgt ttactcccaa
     5581 agttccaatt gtctttttga cagaaactac acacttatgc taaaagatct tttcttgtct
     5641 cgcttcaact ccttaatggt cttgctctct cccccagagc cccgatactc agatgacttg
     5701 atatctcaac tatgccagct gtacattgct ggggatcaag tcttgtctat gtgtggaaac
     5761 tccggctatg aagtcatcaa aatattggag ccatatgtcg tgaatagttt agtccagaga
     5821 gcagaaaagt ttaggcctct cattcattcc ttgggagact ttcctgtatt tataaaagac
     5881 aaggtaagtc aacttgaaga gacgttcggt ccctgtgcaa gaaggttctt tagggctctg
     5941 gatcaattcg acaacataca tgacttggtt tttgtgtttg gctgttacag gcattggggg
     6001 cacccatata tagattatcg aaagggtctg tcaaaactat atgatcaggt tcaccttaaa
     6061 aaaatgatag ataagtccta ccaggagtgc ttagcaagcg acctagccag gaggatcctt
     6121 agatggggtt ttgataagta ctccaagtgg tatctggatt caagattcct agcccgagac
     6181 caccccttga ctccttatat caaaacccaa acatggccac ccaaacatat tgtagacttg
     6241 gtgggggata catggcacaa gctcccgatc acgcagatct ttgagattcc tgaatcaatg
     6301 gatccgtcag aaatattgga tgacaaatca cattctttca ccagaacgag actagcttct
     6361 tggctgtcag aaaaccgagg ggggcctgtt cctagcgaaa aagttattat cacggccctg
     6421 tctaagccgc ctgtcaatcc ccgagagttt ctgaggtcta tagacctcgg aggattgcca
     6481 gatgaagact tgataattgg cctcaagcca aaggaacggg aattgaagat tgaaggtcga
     6541 ttctttgctc taatgtcatg gaatctaaga ttgtattttg tcatcactga aaaactcttg
     6601 gccaactaca tcttgccact ttttgacgcg ctgactatga cagacaacct gaacaaggtg
     6661 tttaaaaagc tgatcgacag ggtcaccggg caagggcttt tggactattc aagggtcaca
     6721 tatgcatttc acctggacta tgaaaagtgg aacaaccatc aaagattaga gtcaacagag
     6781 gatgtatttt ctgtcctaga tcaagtgttt ggattgaaga gagtgttttc tagaacacac
     6841 gagttttttc aaaaggcctg gatctattat tcagacagat cagacctcat cgggttacgg
     6901 gaggatcaaa tatactgctt agatgcgtcc aacggcccaa cctgttggaa tggccaggat
     6961 ggcgggctag aaggcttacg gcagaagggc tggagtctag tcagcttatt gatgatagat
     7021 agagaatctc aaatcaggaa cacaagaacc aaaatactag ctcaaggaga caaccaggtt
     7081 ttatgtccga catacatgtt gtcgccaggg ctatctcaag aggggctcct ctatgaattg
     7141 gagagaatat caaggaatgc actttcgata tacagagccg tcgaggaagg ggcatctaag
     7201 ctagggctga tcatcaagaa agaagagacc atgtgtagtt atgacttcct catctatgga
     7261 aaaacccctt tgtttagagg taacatattg gtgcctgagt ccaaaagatg ggccagagtc
     7321 tcttgcgtct ctaatgacca aatagtcaac ctcgccaata taatgtcgac agtgtccacc
     7381 aatgcgctaa cagtggcaca acactctcaa tctttgatca aaccgatgag ggattttctg
     7441 ctcatgtcag tacaggcagt ctttcactac ctgctattta gcccaatctt aaagggaaga
     7501 gtttacaaga ttctgagcgc tgaaggggag agctttctcc tagccatgtc aaggataatc
     7561 tatctagatc cttctttggg agggatatct ggaatgtccc tcggaagatt ccatatacga
     7621 cagttctcag accctgtctc tgaagggtta tccttctgga gagagatctg gttaagctcc
     7681 caagagtcct ggattcacgc gttgtgtcaa gaggctggaa acccagatct tggagagaga
     7741 acactcgaga gcttcactcg ccttctagaa gatccgacca ccttaaatat cagaggaggg
     7801 gccagtccta ccattctact caaggatgca atcagaaagg ctttatatga cgaggtggac
     7861 aaggtggaaa attcagagtt tcgagaggca atcctgttgt ccaagaccca tagagataat
     7921 tttatactct tcttaatatc tgttgagcct ctgtttcctc gatttctcag tgagctattc
     7981 agttcgtctt ttttgggaat ccccgagtca atcattggat tgatacaaaa ctcccgaacg
     8041 ataagaaggc agtttagaaa gagtctctca aaaactttag aagaatcctt ctacaactca
     8101 gagatccacg ggattagtcg gatgacccag acacctcaga gggttggggg ggtgtggcct
     8161 tgctcttcag agagggcaga tctacttagg gagatctctt ggggaagaaa agtggtaggc
     8221 acgacagttc ctcacccttc tgagatgttg ggattacttc ccaagtcctc tatttcttgc
     8281 acttgtggag caacaggagg aggcaatcct agagtttctg tatcagtact cccgtccttt
     8341 gatcagtcat ttttttcacg aggcccccta aagggatact tgggctcgtc cacctctatg
     8401 tcgacccagc tattccatgc atgggaaaaa gtcactaatg ttcatgtggt gaagagagct
     8461 ctatcgttaa aagaatctat aaactggttc attactagag attccaactt ggctcaagct
     8521 ctaattagga acattatgtc tctgacaggc cctgatttcc ctctagagga ggcccctgtc
     8581 ttcaaaagga cggggtcagc cttgcatagg ttcaagtctg ccagatacag cgaaggaggg
     8641 tattcttctg tctgcccgaa cctcctctct catatttctg ttagtacaga caccatgtct
     8701 gatttgaccc aagacgggaa gaactacgat ttcatgttcc agccattgat gctttatgca
     8761 cagacatgga catcagagct ggtacagaga gacacaaggc taagagactc tacgtttcat
     8821 tggcacctcc gatgcaacag gtgtgtgaga cccattgacg acgtgaccct ggagacctct
     8881 cagatcttcg agtttccgga tgtgtcgaaa agaatatcca gaatggtttc tggggctgtg
     8941 cctcacttcc agaggcttcc cgatatccgt ctgagaccag gagattttga atctctaagc
     9001 ggtagagaaa agtctcacca tatcggatca gctcaggggc tcttatactc aatcttagtg
     9061 gcaattcacg actcaggata caatgatgga accatcttcc ctgtcaacat atacggcaag
     9121 gtttccccta gagactattt gagagggctc gcaaggggag tattgatagg atcctcgatt
     9181 tgcttcttga caagaatgac aaatatcaat attaatagac ctcttgaatt ggtctcaggg
     9241 gtaatctcat atattctcct gaggctagat aaccatccct ccttgtacat aatgctcaga
     9301 gaaccgtctc ttagaggaga gatattttct atccctcaga aaatccccgc cgcttatcca
     9361 accactatga aagaaggcaa cagatcaatc ttgtgttatc tccaacatgt gctacgctat
     9421 gagcgagaga taatcacggc gtctccagag aatgactggc tatggatctt ttcagacttt
     9481 agaagtgcca aaatgacgta cctatccctc attacttacc agtctcatct tctactccag
     9541 agggttgaga gaaacctatc taagagtatg agagataacc tgcgacaatt gagttctttg
     9601 atgaggcagg tgctgggcgg gcacggagaa gataccttag agtcagacga caacattcaa
     9661 cgactgctaa aagactcttt acgaaggaca agatgggtgg atcaagaggt gcgccatgca
     9721 gctagaacca tgactggaga ttacagcccc aacaagaagg tgtcccgtaa ggtaggatgt
     9781 tcagaatggg tctgctctgc tcaacaggtt gcagtctcta cctcagcaaa cccggcccct
     9841 gtctcggagc ttgacataag ggccctctct aagaggttcc agaacccttt gatctcgggc
     9901 ttgagagtgg ttcagtgggc aaccggtgct cattataagc ttaagcctat tctagatgat
     9961 ctcaatgttt tcccatctct ctgccttgta gttggggacg ggtcaggggg gatatcaagg
    10021 gcagtcctca acatgtttcc agatgccaag cttgtgttca acagtctttt agaggtgaat
    10081 gacctgatgg cttccggaac acatccactg cctccttcag caatcatgag gggaggaaat
    10141 gatatcgtct ccagagtgat agatcttgac tcaatctggg aaaaaccgtc cgacttgaga
    10201 aacttggcaa cctggaaata cttccagtca gtccaaaagc aggtcaacat gtcctatgac
    10261 ctcattattt gcgatgcaga agttactgac attgcatcta tcaaccggat caccctgtta
    10321 atgtccgatt ttgcattgtc tatagatgga ccactctatt tggtcttcaa aacttatggg
    10381 actatgctag taaatccaaa ctacaaggct attcaacacc tgtcaagagc gttcccctcg
    10441 gtcacagggt ttatcaccca agtaacttcg tctttttcat ctgagctcta cctccgattc
    10501 tccaaacgag ggaagttttt cagagatgct gagtacttga cctcttccac ccttcgagaa
    10561 atgagccttg tgttattcaa ttgtagcagc cccaagagtg agatgcagag agctcgttcc
    10621 ttgaactatc aggatcttgt gagaggattt cctgaagaaa tcatatcaaa tccttacaat
    10681 gagatgatca taactctgat tgacagtgat gtagaatctt ttctagtcca caagatggtt
    10741 gatgatcttg agttacagag gggaactctg tctaaagtgg ctatcattat agccatcatg
    10801 atagttttct ccaacagagt cttcaacgtt tccaaacccc taactgaccc ctcgttctat
    10861 ccaccgtctg atcccaaaat cctgaggcac ttcaacatat gttgcagtac tatgatgtat
    10921 ctatctactg ctttaggtga cgtccctagc ttcgcaagac ttcacgacct gtataacaga
    10981 cctataactt attacttcag aaagcaagtc attcgaggga acgtttatct atcttggagt
    11041 tggtccaacg acacctcagt gttcaaaagg gtagcctgta attctagcct gagtctgtca
    11101 tctcactgga tcaggttgat ttacaagata gtgaagacta ccagactcgt tggcagcatc
    11161 aaggatctat ccagagaagt ggaaagacac cttcataggt acaacaggtg gatcacccta
    11221 gaggatatca gatctagatc atccctacta gactacagtt gcctgtgaac cggatactcc
    11281 tggaagcctg cccatgctaa gactcttgtg tgatgtatct tgaaaaaaac aagatcctaa
    11341 atctgaacct ttggttgttt gattgttttt ctcatttttg ttgtttattt gttaagcgtg
    11401 ggtcggcatg gcatctccac ctcctcgcgg tccgacctgg gcatccgaag gaggacgcac
    11461 gtccactcgg atggctaagg gagaagggcg aattccagca cactggcggc cgttactagt
    11521 ggatccgagc tcggtaccaa gcttaagttt aaaccgctga tcagcctcga ctgtgccttc
    11581 tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc
    11641 cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg
    11701 tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa
    11761 tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg
    11821 gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
    11881 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
    11941 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
    12001 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg
    12061 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
    12121 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
    12181 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
    12241 gctgatttaa caaaaattta acgcgaatta attctgtgga atgtgtgtca gttagggtgt
    12301 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
    12361 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
    12421 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg
    12481 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
    12541 gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
    12601 ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga tcaagagaca
    12661 ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct
    12721 tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc
    12781 gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc
    12841 ggtgccctga atgaactgca ggacgaggca gcgcggctat cgtggctggc cacgacgggc
    12901 gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg
    12961 ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc
    13021 atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac
    13081 caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat
    13141 caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc
    13201 aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg
    13261 aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg
    13321 gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc
    13381 gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc
    13441 gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc gaaatgaccg
    13501 accaagcgac gcccaacctg ccatcacgag atttcgattc caccgccgcc ttctatgaaa
    13561 ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc
    13621 tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat ggttacaaat
    13681 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
    13741 gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc tctagctaga
    13801 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
    13861 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
    13921 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
    13981 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
    14041 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
    14101 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
    14161 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
    14221 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
    14281 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
    14341 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
    14401 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
    14461 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
    14521 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
    14581 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
    14641 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
    14701 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
    14761 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
    14821 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
    14881 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
    14941 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
    15001 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
    15061 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
    15121 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
    15181 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
    15241 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
    15301 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
    15361 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
    15421 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
    15481 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
    15541 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
    15601 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
    15661 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
    15721 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
    15781 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
    15841 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
    15901 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
    15961 tgccacctga cgtcgacgga tcgggagatc tcccgatccc ctatggtgca ctctcagtac
    16021 aatctgctct gatgccgcat agttaagcca gtatctgctc cctgcttgtg tgttggaggt
    16081 cgctgagtag tgcgcgagca aaatttaagc tacaacaagg caaggcttga ccgacaattg
    16141 catgaagaat ctgcttaggg ttaggcgttt tgcgctgctt cgcgatgtac gggccagata
    16201 tacgcgtt
//
