LOCUS       sequence-137831-       16270 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16270)
  AUTHORS   Osakada F, Mori T, Cetin AH, Marshel JH, Virgen B, Callaway EM
  TITLE     New rabies virus variants for monitoring and manipulating activity 
            and gene expression in defined neural circuits.
  JOURNAL   Neuron. 2011 Aug 25;71(4):617-31.
  PUBMED    21867879
REFERENCE   2  (bases 1 to 16270)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron."; 
            date: "2011-08-25"; volume: "71(4)"; pages: "617-31"
COMMENT     Sequence Label: pSADdeltaG-DsRed2
FEATURES             Location/Qualifiers
     source          1..16270
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     629..648
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      4142..4151
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4148..4825
                     /codon_start=1
                     /product="rapidly maturing tetrameric variant of DsRed 
                     fluorescent protein (Bevis and Glick, 2002)"
                     /label=DsRed-Express
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGSFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERAEGRHH
                     LFL"
     primer_bind     complement(4278..4296)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     complement(4328..4348)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     4546..4565
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     4740..4763
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     CDS             6591..6602
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(11627..11644)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    11633..11857
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      11903..12331
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11990..12009)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     12200..12221
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(12340..12360)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        12345..12674
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      12525..12660
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     12587..12606
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             12741..13535
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(12795..12814)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     13405..13424
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    13709..13830
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(13746..13765)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     13800..13819
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(13879..13895)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13879..13895)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(13892..13914)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    13903..13919
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(13927..13933,13934..13951,13952..13957))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13972..13993
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(14110..14127)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(14281..14869)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(14361..14380)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(15040..15831,15832..15900))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     15663..15682
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(15901..16005)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(16080..16099)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctg
      661 gctagcgttt aaacgggccc tctagactcg agcggccgcc agtgtgatgg atatctgcag
      721 aattcgccct tttaagcgtc tgatgagtcc gtgaggacga aacccggagt cccgggtcac
      781 gcttaacaac cagatcaaag aaaaaacaga cattgtcaat tgcaaagcaa aaatgtaaca
      841 cccctacaat ggatgccgac aagattgtat tcaaagtcaa taatcaggtg gtctctttga
      901 agcctgagat tatcgtggat caatatgagt acaagtaccc tgccatcaaa gatttgaaaa
      961 agccctgtat aaccctagga aaggctcccg atttaaataa agcatacaag tcagttttgt
     1021 caggcatgag cgccgccaaa cttaatcctg acgatgtatg ttcctatttg gcagcggcaa
     1081 tgcagttttt tgaggggaca tgtccggaag actggaccag ctatggaatt gtgattgcac
     1141 gaaaaggaga taagatcacc ccaggttctc tggtggagat aaaacgtact gatgtagaag
     1201 ggaattgggc tctgacagga ggcatggaac tgacaagaga ccccactgtc cctgagcatg
     1261 cgtccttagt cggtcttctc ttgagtctgt ataggttgag caaaatatcc gggcaaaaca
     1321 ctggtaacta taagacaaac attgcagaca ggatagagca gatttttgag acagcccctt
     1381 ttgttaaaat cgtggaacac catactctaa tgacaactca caaaatgtgt gctaattgga
     1441 gtactatacc aaacttcaga tttttggccg gaacctatga catgtttttc tcccggattg
     1501 agcatctata ttcagcaatc agagtgggca cagttgtcac tgcttatgaa gactgttcag
     1561 gactggtatc atttactggg ttcataaaac aaatcaatct caccgctaga gaggcaatac
     1621 tatatttctt ccacaagaac tttgaggaag agataagaag aatgtttgag ccagggcagg
     1681 agacagctgt tcctcactct tatttcatcc acttccgttc actaggcttg agtgggaaat
     1741 ctccttattc atcaaatgct gttggtcacg tgttcaatct cattcacttt gtaggatgct
     1801 atatgggtca agtcagatcc ctaaatgcaa cggttattgc tgcatgtgct cctcatgaaa
     1861 tgtctgttct agggggctat ctgggagagg aattcttcgg gaaagggaca tttgaaagaa
     1921 gattcttcag agatgagaaa gaacttcaag aatacgaggc ggctgaactg acaaagactg
     1981 acgtagcact ggcagatgat ggaactgtca actctgacga cgaggactac ttttcaggtg
     2041 aaaccagaag tccggaggct gtttatactc gaatcatgat gaatggaggt cgactaaaga
     2101 gatctcacat acggagatat gtctcagtca gttccaatca tcaagcccgt ccaaactcat
     2161 tcgccgagtt tctaaacaag acatattcga gtgactcata agaagttgaa taacaaaatg
     2221 ccggaaatct acggattgtg tatatccatc atgaaaaaaa ctaacacccc tcctttcgaa
     2281 ccatcccaaa catgagcaag atctttgtca atcctagtgc tattagagcc ggtctggccg
     2341 atcttgagat ggctgaagaa actgttgatc tgatcaatag aaatatcgaa gacaatcagg
     2401 ctcatctcca aggggaaccc atagaggtgg acaatctccc tgaggatatg gggcgacttc
     2461 acctggatga tggaaaatcg cccaaccatg gtgagatagc caaggtggga gaaggcaagt
     2521 atcgagagga ctttcagatg gatgaaggag aggatcctag cttcctgttc cagtcatacc
     2581 tggaaaatgt tggagtccaa atagtcagac aaatgaggtc aggagagaga tttctcaaga
     2641 tatggtcaca gaccgtagaa gagattatat cctatgtcgc ggtcaacttt cccaaccctc
     2701 caggaaagtc ttcagaggat aaatcaaccc agactactgg ccgagagctc aagaaggaga
     2761 caacacccac tccttctcag agagaaagcc aatcatcgaa agccaggatg gcggctcaaa
     2821 ttgcttctgg ccctccagcc cttgaatggt cggctaccaa tgaagaggat gatctatcag
     2881 tggaggctga gatcgctcac cagattgcag aaagtttctc caaaaaatat aagtttccct
     2941 ctcgatcctc agggatactc ttgtataatt ttgagcaatt gaaaatgaac cttgatgata
     3001 tagttaaaga ggcaaaaaat gtaccaggtg tgacccgttt agcccatgac gggtccaaac
     3061 tccccctaag atgtgtactg ggatgggtcg ctttggccaa ctctaagaaa ttccagttgt
     3121 tagtcgaatc cgacaagctg agtaaaatca tgcaagatga cttgaatcgc tatacatctt
     3181 gctaaccgaa cctctcccct cagtccctct agacaataaa atccgagatg tcccaaagtc
     3241 aacatgaaaa aaacaggcaa caccactgat aaaatgaacc tcctacgtaa gatagtgaaa
     3301 aaccgcaggg acgaggacac tcaaaaatcc tctcccgcgt cagcccctct ggatgacgat
     3361 gacttgtggc ttccaccccc tgaatacgtc ccgctgaaag aacttacagg caagaagaac
     3421 atgaggaact tttgtatcaa cggaagggtt aaagtgtgta gcccgaatgg ttactcgttc
     3481 aggatcctgc ggcacattct gaaatcattc gacgagatat attctgggaa tcataggatg
     3541 atcgggttag tcaaagtggt tattggactg gctttgtcag gatctccagt ccctgagggc
     3601 ctgaactggg tatacaaatt gaggagaacc tttatcttcc agtgggctga ttccaggggc
     3661 cctcttgaag gggaggagtt ggaatactct caggagatca cttgggatga tgatactgag
     3721 ttcgtcggat tgcaaataag agtgattgca aaacagtgtc atatccaggg cagagtctgg
     3781 tgtatcaaca tgaacccgag agcatgtcaa ctatggtctg acatgtctct tcagacacaa
     3841 aggtccgaag aggacaaaga ttcctctctg cttctagaat aatcagatta tatcccgcaa
     3901 atttatcact tgtttacctc tggaggagag aacatatggg ctcaactcca acccttggga
     3961 gcaatataac aaaaaacatg ttatggtgcc attaaaccgc tgcatttcat caaagtcaag
     4021 ttgattacct ttacattttg atcctcttgg atgtgaaaaa aactattaac atccctcaaa
     4081 ggacgggatc caccggcccg ggcccgggcc cgggaattca tttgatcccc gggtaccggt
     4141 cgccaccatg gcctcctccg aggacgtcat caaggagttc atgcgcttca aggtgcgcat
     4201 ggagggctcc gtgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta
     4261 cgagggcacc cagaccgcca agctgaaggt gaccaagggc ggccccctgc ccttcgcctg
     4321 ggacatcctg tccccccagt tccagtacgg ctccaaggtg tacgtgaagc accccgccga
     4381 catccccgac tacaagaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa
     4441 cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg acggctcctt
     4501 catctacaag gtgaagttca tcggcgtgaa cttcccctcc gacggccccg taatgcagaa
     4561 gaagactatg ggctgggagg cctccaccga gcgcctgtac ccccgcgacg gcgtgctgaa
     4621 gggcgagatc cacaaggccc tgaagctgaa ggacggcggc cactacctgg tggagttcaa
     4681 gtccatctac atggccaaga agcccgtgca gctgcccggc tactactacg tggactccaa
     4741 gctggacatc acctcccaca acgaggacta caccatcgtg gagcagtacg agcgcgccga
     4801 gggccgccac cacctgttcc tgtagcggcc aaatgaattc ccggggtaca agtaaagcgg
     4861 ccgacccgct agcttttcag tcgagaaaaa aacattagat cagaagaaca actggcaaca
     4921 cttctcaacc tgagacttac ttcaagatgc tcgatcctgg agaggtctat gatgacccta
     4981 ttgacccaat cgagttagag gctgaaccca gaggaacccc cattgtcccc aacatcttga
     5041 ggaactctga ctacaatctc aactctcctt tgatagaaga tcctgctaga ctaatgttag
     5101 aatggttaaa aacagggaat agaccttatc ggatgactct aacagacaat tgctccaggt
     5161 ctttcagagt tttgaaagat tatttcaaga aggtagattt gggttctctc aaggtgggcg
     5221 gaatggctgc acagtcaatg atttctctct ggttatatgg tgcccactct gaatccaaca
     5281 ggagccggag atgtataaca gacttggccc atttctattc caagtcgtcc cccatagaga
     5341 agctgttgaa tctcacgcta ggaaatagag ggctgagaat ccccccagag ggagtgttaa
     5401 gttgccttga gagggttgat tatgataatg catttggaag gtatcttgcc aacacgtatt
     5461 cctcttactt gttcttccat gtaatcacct tatacatgaa cgccctagac tgggatgaag
     5521 aaaagaccat cctagcatta tggaaagatt taacctcagt ggacatcggg aaggacttgg
     5581 taaagttcaa agaccaaata tggggactgc tgatcgtgac aaaggacttt gtttactccc
     5641 aaagttccaa ttgtcttttt gacagaaact acacacttat gctaaaagat cttttcttgt
     5701 ctcgcttcaa ctccttaatg gtcttgctct ctcccccaga gccccgatac tcagatgact
     5761 tgatatctca actatgccag ctgtacattg ctggggatca agtcttgtct atgtgtggaa
     5821 actccggcta tgaagtcatc aaaatattgg agccatatgt cgtgaatagt ttagtccaga
     5881 gagcagaaaa gtttaggcct ctcattcatt ccttgggaga ctttcctgta tttataaaag
     5941 acaaggtaag tcaacttgaa gagacgttcg gtccctgtgc aagaaggttc tttagggctc
     6001 tggatcaatt cgacaacata catgacttgg tttttgtgtt tggctgttac aggcattggg
     6061 ggcacccata tatagattat cgaaagggtc tgtcaaaact atatgatcag gttcacctta
     6121 aaaaaatgat agataagtcc taccaggagt gcttagcaag cgacctagcc aggaggatcc
     6181 ttagatgggg ttttgataag tactccaagt ggtatctgga ttcaagattc ctagcccgag
     6241 accacccctt gactccttat atcaaaaccc aaacatggcc acccaaacat attgtagact
     6301 tggtggggga tacatggcac aagctcccga tcacgcagat ctttgagatt cctgaatcaa
     6361 tggatccgtc agaaatattg gatgacaaat cacattcttt caccagaacg agactagctt
     6421 cttggctgtc agaaaaccga ggggggcctg ttcctagcga aaaagttatt atcacggccc
     6481 tgtctaagcc gcctgtcaat ccccgagagt ttctgaggtc tatagacctc ggaggattgc
     6541 cagatgaaga cttgataatt ggcctcaagc caaaggaacg ggaattgaag attgaaggtc
     6601 gattctttgc tctaatgtca tggaatctaa gattgtattt tgtcatcact gaaaaactct
     6661 tggccaacta catcttgcca ctttttgacg cgctgactat gacagacaac ctgaacaagg
     6721 tgtttaaaaa gctgatcgac agggtcaccg ggcaagggct tttggactat tcaagggtca
     6781 catatgcatt tcacctggac tatgaaaagt ggaacaacca tcaaagatta gagtcaacag
     6841 aggatgtatt ttctgtccta gatcaagtgt ttggattgaa gagagtgttt tctagaacac
     6901 acgagttttt tcaaaaggcc tggatctatt attcagacag atcagacctc atcgggttac
     6961 gggaggatca aatatactgc ttagatgcgt ccaacggccc aacctgttgg aatggccagg
     7021 atggcgggct agaaggctta cggcagaagg gctggagtct agtcagctta ttgatgatag
     7081 atagagaatc tcaaatcagg aacacaagaa ccaaaatact agctcaagga gacaaccagg
     7141 ttttatgtcc gacatacatg ttgtcgccag ggctatctca agaggggctc ctctatgaat
     7201 tggagagaat atcaaggaat gcactttcga tatacagagc cgtcgaggaa ggggcatcta
     7261 agctagggct gatcatcaag aaagaagaga ccatgtgtag ttatgacttc ctcatctatg
     7321 gaaaaacccc tttgtttaga ggtaacatat tggtgcctga gtccaaaaga tgggccagag
     7381 tctcttgcgt ctctaatgac caaatagtca acctcgccaa tataatgtcg acagtgtcca
     7441 ccaatgcgct aacagtggca caacactctc aatctttgat caaaccgatg agggattttc
     7501 tgctcatgtc agtacaggca gtctttcact acctgctatt tagcccaatc ttaaagggaa
     7561 gagtttacaa gattctgagc gctgaagggg agagctttct cctagccatg tcaaggataa
     7621 tctatctaga tccttctttg ggagggatat ctggaatgtc cctcggaaga ttccatatac
     7681 gacagttctc agaccctgtc tctgaagggt tatccttctg gagagagatc tggttaagct
     7741 cccaagagtc ctggattcac gcgttgtgtc aagaggctgg aaacccagat cttggagaga
     7801 gaacactcga gagcttcact cgccttctag aagatccgac caccttaaat atcagaggag
     7861 gggccagtcc taccattcta ctcaaggatg caatcagaaa ggctttatat gacgaggtgg
     7921 acaaggtgga aaattcagag tttcgagagg caatcctgtt gtccaagacc catagagata
     7981 attttatact cttcttaata tctgttgagc ctctgtttcc tcgatttctc agtgagctat
     8041 tcagttcgtc ttttttggga atccccgagt caatcattgg attgatacaa aactcccgaa
     8101 cgataagaag gcagtttaga aagagtctct caaaaacttt agaagaatcc ttctacaact
     8161 cagagatcca cgggattagt cggatgaccc agacacctca gagggttggg ggggtgtggc
     8221 cttgctcttc agagagggca gatctactta gggagatctc ttggggaaga aaagtggtag
     8281 gcacgacagt tcctcaccct tctgagatgt tgggattact tcccaagtcc tctatttctt
     8341 gcacttgtgg agcaacagga ggaggcaatc ctagagtttc tgtatcagta ctcccgtcct
     8401 ttgatcagtc atttttttca cgaggccccc taaagggata cttgggctcg tccacctcta
     8461 tgtcgaccca gctattccat gcatgggaaa aagtcactaa tgttcatgtg gtgaagagag
     8521 ctctatcgtt aaaagaatct ataaactggt tcattactag agattccaac ttggctcaag
     8581 ctctaattag gaacattatg tctctgacag gccctgattt ccctctagag gaggcccctg
     8641 tcttcaaaag gacggggtca gccttgcata ggttcaagtc tgccagatac agcgaaggag
     8701 ggtattcttc tgtctgcccg aacctcctct ctcatatttc tgttagtaca gacaccatgt
     8761 ctgatttgac ccaagacggg aagaactacg atttcatgtt ccagccattg atgctttatg
     8821 cacagacatg gacatcagag ctggtacaga gagacacaag gctaagagac tctacgtttc
     8881 attggcacct ccgatgcaac aggtgtgtga gacccattga cgacgtgacc ctggagacct
     8941 ctcagatctt cgagtttccg gatgtgtcga aaagaatatc cagaatggtt tctggggctg
     9001 tgcctcactt ccagaggctt cccgatatcc gtctgagacc aggagatttt gaatctctaa
     9061 gcggtagaga aaagtctcac catatcggat cagctcaggg gctcttatac tcaatcttag
     9121 tggcaattca cgactcagga tacaatgatg gaaccatctt ccctgtcaac atatacggca
     9181 aggtttcccc tagagactat ttgagagggc tcgcaagggg agtattgata ggatcctcga
     9241 tttgcttctt gacaagaatg acaaatatca atattaatag acctcttgaa ttggtctcag
     9301 gggtaatctc atatattctc ctgaggctag ataaccatcc ctccttgtac ataatgctca
     9361 gagaaccgtc tcttagagga gagatatttt ctatccctca gaaaatcccc gccgcttatc
     9421 caaccactat gaaagaaggc aacagatcaa tcttgtgtta tctccaacat gtgctacgct
     9481 atgagcgaga gataatcacg gcgtctccag agaatgactg gctatggatc ttttcagact
     9541 ttagaagtgc caaaatgacg tacctatccc tcattactta ccagtctcat cttctactcc
     9601 agagggttga gagaaaccta tctaagagta tgagagataa cctgcgacaa ttgagttctt
     9661 tgatgaggca ggtgctgggc gggcacggag aagatacctt agagtcagac gacaacattc
     9721 aacgactgct aaaagactct ttacgaagga caagatgggt ggatcaagag gtgcgccatg
     9781 cagctagaac catgactgga gattacagcc ccaacaagaa ggtgtcccgt aaggtaggat
     9841 gttcagaatg ggtctgctct gctcaacagg ttgcagtctc tacctcagca aacccggccc
     9901 ctgtctcgga gcttgacata agggccctct ctaagaggtt ccagaaccct ttgatctcgg
     9961 gcttgagagt ggttcagtgg gcaaccggtg ctcattataa gcttaagcct attctagatg
    10021 atctcaatgt tttcccatct ctctgccttg tagttgggga cgggtcaggg gggatatcaa
    10081 gggcagtcct caacatgttt ccagatgcca agcttgtgtt caacagtctt ttagaggtga
    10141 atgacctgat ggcttccgga acacatccac tgcctccttc agcaatcatg aggggaggaa
    10201 atgatatcgt ctccagagtg atagatcttg actcaatctg ggaaaaaccg tccgacttga
    10261 gaaacttggc aacctggaaa tacttccagt cagtccaaaa gcaggtcaac atgtcctatg
    10321 acctcattat ttgcgatgca gaagttactg acattgcatc tatcaaccgg atcaccctgt
    10381 taatgtccga ttttgcattg tctatagatg gaccactcta tttggtcttc aaaacttatg
    10441 ggactatgct agtaaatcca aactacaagg ctattcaaca cctgtcaaga gcgttcccct
    10501 cggtcacagg gtttatcacc caagtaactt cgtctttttc atctgagctc tacctccgat
    10561 tctccaaacg agggaagttt ttcagagatg ctgagtactt gacctcttcc acccttcgag
    10621 aaatgagcct tgtgttattc aattgtagca gccccaagag tgagatgcag agagctcgtt
    10681 ccttgaacta tcaggatctt gtgagaggat ttcctgaaga aatcatatca aatccttaca
    10741 atgagatgat cataactctg attgacagtg atgtagaatc ttttctagtc cacaagatgg
    10801 ttgatgatct tgagttacag aggggaactc tgtctaaagt ggctatcatt atagccatca
    10861 tgatagtttt ctccaacaga gtcttcaacg tttccaaacc cctaactgac ccctcgttct
    10921 atccaccgtc tgatcccaaa atcctgaggc acttcaacat atgttgcagt actatgatgt
    10981 atctatctac tgctttaggt gacgtcccta gcttcgcaag acttcacgac ctgtataaca
    11041 gacctataac ttattacttc agaaagcaag tcattcgagg gaacgtttat ctatcttgga
    11101 gttggtccaa cgacacctca gtgttcaaaa gggtagcctg taattctagc ctgagtctgt
    11161 catctcactg gatcaggttg atttacaaga tagtgaagac taccagactc gttggcagca
    11221 tcaaggatct atccagagaa gtggaaagac accttcatag gtacaacagg tggatcaccc
    11281 tagaggatat cagatctaga tcatccctac tagactacag ttgcctgtga accggatact
    11341 cctggaagcc tgcccatgct aagactcttg tgtgatgtat cttgaaaaaa acaagatcct
    11401 aaatctgaac ctttggttgt ttgattgttt ttctcatttt tgttgtttat ttgttaagcg
    11461 tgggtcggca tggcatctcc acctcctcgc ggtccgacct gggcatccga aggaggacgc
    11521 acgtccactc ggatggctaa gggagaaggg cgaattccag cacactggcg gccgttacta
    11581 gtggatccga gctcggtacc aagcttaagt ttaaaccgct gatcagcctc gactgtgcct
    11641 tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt
    11701 gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg
    11761 tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac
    11821 aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc
    11881 tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
    11941 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
    12001 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
    12061 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
    12121 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
    12181 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
    12241 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
    12301 gagctgattt aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt cagttagggt
    12361 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
    12421 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
    12481 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc
    12541 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
    12601 ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
    12661 taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct gatcaagaga
    12721 caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg
    12781 cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg
    12841 ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt
    12901 ccggtgccct gaatgaactg caggacgagg cagcgcggct atcgtggctg gccacgacgg
    12961 gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat
    13021 tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat
    13081 ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg
    13141 accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg
    13201 atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc
    13261 tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc
    13321 cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg
    13381 tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg
    13441 gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca
    13501 tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt tcgaaatgac
    13561 cgaccaagcg acgcccaacc tgccatcacg agatttcgat tccaccgccg ccttctatga
    13621 aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga
    13681 tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa
    13741 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
    13801 tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta
    13861 gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
    13921 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
    13981 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
    14041 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
    14101 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
    14161 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
    14221 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
    14281 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
    14341 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
    14401 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
    14461 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
    14521 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
    14581 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
    14641 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
    14701 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
    14761 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
    14821 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
    14881 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
    14941 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
    15001 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
    15061 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
    15121 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
    15181 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
    15241 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
    15301 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
    15361 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
    15421 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
    15481 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
    15541 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
    15601 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
    15661 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
    15721 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
    15781 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
    15841 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
    15901 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
    15961 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
    16021 agtgccacct gacgtcgacg gatcgggaga tctcccgatc ccctatggtg cactctcagt
    16081 acaatctgct ctgatgccgc atagttaagc cagtatctgc tccctgcttg tgtgttggag
    16141 gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt gaccgacaat
    16201 tgcatgaaga atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga
    16261 tatacgcgtt
//
