LOCUS       sequence-137832-       15943 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15943)
  AUTHORS   Osakada F, Mori T, Cetin AH, Marshel JH, Virgen B, Callaway EM
  TITLE     New rabies virus variants for monitoring and manipulating activity 
            and gene expression in defined neural circuits.
  JOURNAL   Neuron. 2011 Aug 25;71(4):617-31.
  PUBMED    21867879
REFERENCE   2  (bases 1 to 15943)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron."; 
            date: "2011-08-25"; volume: "71(4)"; pages: "617-31"
COMMENT     Sequence Label: pSADdeltaG-BFP
FEATURES             Location/Qualifiers
     source          1..15943
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     629..648
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             4091..4789
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     misc_feature    4790..4855
                     /label=MCS
                     /note="multiple cloning site"
     CDS             6608..6619
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(11717..11734)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    11723..11947
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      11993..12421
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(12080..12099)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     12290..12311
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(12430..12450)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        12435..12764
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      12615..12750
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     12677..12696
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        12812..12859
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             12878..13252
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    13382..13503
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(13419..13438)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     13473..13492
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(13552..13568)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13552..13568)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(13565..13587)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    13576..13592
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(13600..13606,13607..13624,13625..13630))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13645..13666
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(13783..13800)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(13954..14542)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(14034..14053)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(14713..15504,15505..15573))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     15336..15355
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(15574..15678)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(15753..15772)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctg
      661 gctagattaa gcgtctgatg agtccgtgag gacgaaaccc ggagtcccgg gtcacgctta
      721 acaaccagat caaagaaaaa acagacattg tcaattgcaa agcaaaaatg taacacccct
      781 acaatggatg ccgacaagat tgtattcaaa gtcaataatc aggtggtctc tttgaagcct
      841 gagattatcg tggatcaata tgagtacaag taccctgcca tcaaagattt gaaaaagccc
      901 tgtataaccc taggaaaggc tcccgattta aataaagcat acaagtcagt tttgtcaggc
      961 atgagcgccg ccaaacttaa tcctgacgat gtatgttcct atttggcagc ggcaatgcag
     1021 ttttttgagg ggacatgtcc ggaagactgg accagctatg gaattgtgat tgcacgaaaa
     1081 ggagataaga tcaccccagg ttctctggtg gagataaaac gtactgatgt agaagggaat
     1141 tgggctctga caggaggcat ggaactgaca agagacccca ctgtccctga gcatgcgtcc
     1201 ttagtcggtc ttctcttgag tctgtatagg ttgagcaaaa tatccgggca aaacactggt
     1261 aactataaga caaacattgc agacaggata gagcagattt ttgagacagc cccttttgtt
     1321 aaaatcgtgg aacaccatac tctaatgaca actcacaaaa tgtgtgctaa ttggagtact
     1381 ataccaaact tcagattttt ggccggaacc tatgacatgt ttttctcccg gattgagcat
     1441 ctatattcag caatcagagt gggcacagtt gtcactgctt atgaagactg ttcaggactg
     1501 gtatcattta ctgggttcat aaaacaaatc aatctcaccg ctagagaggc aatactatat
     1561 ttcttccaca agaactttga ggaagagata agaagaatgt ttgagccagg gcaggagaca
     1621 gctgttcctc actcttattt catccacttc cgttcactag gcttgagtgg gaaatctcct
     1681 tattcatcaa atgctgttgg tcacgtgttc aatctcattc actttgtagg atgctatatg
     1741 ggtcaagtca gatccctaaa tgcaacggtt attgctgcat gtgctcctca tgaaatgtct
     1801 gttctagggg gctatctggg agaggaattc ttcgggaaag ggacatttga aagaagattc
     1861 ttcagagatg agaaagaact tcaagaatac gaggcggctg aactgacaaa gactgacgta
     1921 gcactggcag atgatggaac tgtcaactct gacgacgagg actacttttc aggtgaaacc
     1981 agaagtccgg aggctgttta tactcgaatc atgatgaatg gaggtcgact aaagagatct
     2041 cacatacgga gatatgtctc agtcagttcc aatcatcaag cccgtccaaa ctcattcgcc
     2101 gagtttctaa acaagacata ttcgagtgac tcataagaag ttgaataaca aaatgccgga
     2161 aatctacgga ttgtgtatat ccatcatgaa aaaaactaac acccctcctt tcgaaccatc
     2221 ccaaacatga gcaagatctt tgtcaatcct agtgctatta gagccggtct ggccgatctt
     2281 gagatggctg aagaaactgt tgatctgatc aatagaaata tcgaagacaa tcaggctcat
     2341 ctccaagggg aacccataga ggtggacaat ctccctgagg atatggggcg acttcacctg
     2401 gatgatggaa aatcgcccaa ccatggtgag atagccaagg tgggagaagg caagtatcga
     2461 gaggactttc agatggatga aggagaggat cctagcttcc tgttccagtc atacctggaa
     2521 aatgttggag tccaaatagt cagacaaatg aggtcaggag agagatttct caagatatgg
     2581 tcacagaccg tagaagagat tatatcctat gtcgcggtca actttcccaa ccctccagga
     2641 aagtcttcag aggataaatc aacccagact actggccgag agctcaagaa ggagacaaca
     2701 cccactcctt ctcagagaga aagccaatca tcgaaagcca ggatggcggc tcaaattgct
     2761 tctggccctc cagcccttga atggtcggct accaatgaag aggatgatct atcagtggag
     2821 gctgagatcg ctcaccagat tgcagaaagt ttctccaaaa aatataagtt tccctctcga
     2881 tcctcaggga tactcttgta taattttgag caattgaaaa tgaaccttga tgatatagtt
     2941 aaagaggcaa aaaatgtacc aggtgtgacc cgtttagccc atgacgggtc caaactcccc
     3001 ctaagatgtg tactgggatg ggtcgctttg gccaactcta agaaattcca gttgttagtc
     3061 gaatccgaca agctgagtaa aatcatgcaa gatgacttga atcgctatac atcttgctaa
     3121 ccgaacctct cccctcagtc cctctagaca ataaaatccg agatgtccca aagtcaacat
     3181 gaaaaaaaca ggcaacacca ctgataaatc gatgaacctc ctacgtaaga tagtgaaaaa
     3241 ccgcagggac gaggacactc aaaaatcctc tcccgcgtca gcccctctgg atgacgatga
     3301 cttgtggctt ccaccccctg aatacgtccc gctgaaagaa cttacaggca agaagaacat
     3361 gaggaacttt tgtatcaacg gaagggttaa agtgtgtagc ccgaatggtt actcgttcag
     3421 gatcctgcgg cacattctga aatcattcga cgagatatat tctgggaatc ataggatgat
     3481 cgggttagtc aaagtggtta ttggactggc tttgtcagga tctccagtcc ctgagggcct
     3541 gaactgggta tacaaattga ggagaacctt tatcttccag tgggctgatt ccaggggccc
     3601 tcttgaaggg gaggagttgg aatactctca ggagatcact tgggatgatg atactgagtt
     3661 cgtcggattg caaataagag tgattgcaaa acagtgtcat atccagggca gagtctggtg
     3721 tatcaacatg aacccgagag catgtcaact atggtctgac atgtctcttc agacacaaag
     3781 gtccgaagag gacaaagatt cctctctgct tctagaataa cgtacgtcag attatatccc
     3841 gcaaatttat cacttgttta cctctggagg agagaacata tgggctcaac tccaaccctt
     3901 gggagcaata taacaaaaaa catgttatgg tgccattaaa ccgctgcatt tcatcaaagt
     3961 caagttgatt acctttacat tttgatcctc ttggatgtga aaaaaactat taacatccct
     4021 caaaggacct gcaggatgcc cgggcatgct agcatgaaaa aaactaacac ccctccttaa
     4081 ttaagccacc atgagcgagc tgattaagga gaacatgcac atgaagctgt acatggaggg
     4141 caccgtggac aaccatcact tcaagtgcac atccgagggc gaaggcaagc cctacgaggg
     4201 cacccagacc atgagaatca aggtggtcga gggcggccct ctccccttcg ccttcgacat
     4261 cctggctact agcttcctct acggcagcaa gaccttcatc aaccacaccc agggcatccc
     4321 cgacttcttc aagcagtcct tccctgaggg cttcacatgg gagagagtca ccacatacga
     4381 agacgggggc gtgctgaccg ctacccagga caccagcctc caggacggct gcctcatcta
     4441 caacgtcaag atcagagggg tgaacttcac atccaacggc cctgtgatgc agaagaaaac
     4501 actcggctgg gaggccttca ccgagacgct gtaccccgct gacggcggcc tggaaggcag
     4561 aaacgacatg gccctgaagc tcgtgggcgg gagccatctg atcgcaaaca tcaagaccac
     4621 atatagatcc aagaaacccg ctaagaacct caagatgcct ggcgtctact atgtggacta
     4681 cagactggaa agaatcaagg aggccaacaa cgagacctac gtcgagcagc acgaggtggc
     4741 agtggccaga tactgcgacc tccctagcaa actggggcac aagcttaatt ccggactcag
     4801 atctcgagct caagcttcga attctgcagt cgacggtacc gcgggcccgg gatccaccgg
     4861 atctagataa ggcgcgccat ccgcggtagc ttttcagtcg agaaaaaaac attagatcag
     4921 aagaacaact ggcaacactt ctcaacctga gacttacttc aagatgctcg atcctggaga
     4981 ggtctatgat gaccctattg acccaatcga gttagaggct gaacccagag gaacccccat
     5041 tgtccccaac atcttgagga actctgacta caatctcaac tctcctttga tagaagatcc
     5101 tgctagacta atgttagaat ggttaaaaac agggaataga ccttatcgga tgactctaac
     5161 agacaattgc tccaggtctt tcagagtttt gaaagattat ttcaagaagg tagatttggg
     5221 ttctctcaag gtgggcggaa tggctgcaca gtcaatgatt tctctctggt tatatggtgc
     5281 ccactctgaa tccaacagga gccggagatg tataacagac ttggcccatt tctattccaa
     5341 gtcgtccccc atagagaagc tgttgaatct cacgctagga aatagagggc tgagaatccc
     5401 cccagaggga gtgttaagtt gccttgagag ggttgattat gataatgcat ttggaaggta
     5461 tcttgccaac acgtattcct cttacttgtt cttccatgta atcaccttat acatgaacgc
     5521 cctagactgg gatgaagaaa agaccatcct agcattatgg aaagatttaa cctcagtgga
     5581 catcgggaag gacttggtaa agttcaaaga ccaaatatgg ggactgctga tcgtgacaaa
     5641 ggactttgtt tactcccaaa gttccaattg tctttttgac agaaactaca cacttatgct
     5701 aaaagatctt ttcttgtctc gcttcaactc cttaatggtc ttgctctctc ccccagagcc
     5761 ccgatactca gatgacttga tatctcaact atgccagctg tacattgctg gggatcaagt
     5821 cttgtctatg tgtggaaact ccggctatga agtcatcaaa atattggagc catatgtcgt
     5881 gaatagttta gtccagagag cagaaaagtt taggcctctc attcattcct tgggagactt
     5941 tcctgtattt ataaaagaca aggtaagtca acttgaagag acgttcggtc cctgtgcaag
     6001 aaggttcttt agggctctgg atcaattcga caacatacat gacttggttt ttgtgtttgg
     6061 ctgttacagg cattgggggc acccatatat agattatcga aagggtctgt caaaactata
     6121 tgatcaggtt caccttaaaa aaatgataga taagtcctac caggagtgct tagcaagcga
     6181 cctagccagg aggatcctta gatggggttt tgataagtac tccaagtggt atctggattc
     6241 aagattccta gcccgagacc accccttgac tccttatatc aaaacccaaa catggccacc
     6301 caaacatatt gtagacttgg tgggggatac atggcacaag ctcccgatca cgcagatctt
     6361 tgagattcct gaatcaatgg atccgtcaga aatattggat gacaaatcac attctttcac
     6421 cagaacgaga ctagcttctt ggctgtcaga aaaccgaggg gggcctgttc ctagcgaaaa
     6481 agttattatc acggccctgt ctaagccgcc tgtcaatccc cgagagtttc tgaggtctat
     6541 agacctcgga ggattgccag atgaagactt gataattggc ctcaagccaa aggaacggga
     6601 attgaagatt gaaggtcgat tctttgctct aatgtcatgg aatctaagat tgtattttgt
     6661 catcactgaa aaactcttgg ccaactacat cttgccactt tttgacgcgc tgactatgac
     6721 agacaacctg aacaaggtgt ttaaaaagct gatcgacagg gtcaccgggc aagggctttt
     6781 ggactattca agggtcacat atgcatttca cctggactat gaaaagtgga acaaccatca
     6841 aagattagag tcaacagagg atgtattttc tgtcctagat caagtgtttg gattgaagag
     6901 agtgttttct agaacacacg agttttttca aaaggcctgg atctattatt cagacagatc
     6961 agacctcatc gggttacggg aggatcaaat atactgctta gatgcgtcca acggcccaac
     7021 ctgttggaat ggccaggatg gcgggctaga aggcttacgg cagaagggct ggagtctagt
     7081 cagcttattg atgatagata gagaatctca aatcaggaac acaagaacca aaatactagc
     7141 tcaaggagac aaccaggttt tatgtccgac atacatgttg tcgccagggc tatctcaaga
     7201 ggggctcctc tatgaattgg agagaatatc aaggaatgca ctttcgatat acagagccgt
     7261 cgaggaaggg gcatctaagc tagggctgat catcaagaaa gaagagacca tgtgtagtta
     7321 tgacttcctc atctatggaa aaaccccttt gtttagaggt aacatattgg tgcctgagtc
     7381 caaaagatgg gccagagtct cttgcgtctc taatgaccaa atagtcaacc tcgccaatat
     7441 aatgtcgaca gtgtccacca atgcgctaac agtggcacaa cactctcaat ctttgatcaa
     7501 accgatgagg gattttctgc tcatgtcagt acaggcagtc tttcactacc tgctatttag
     7561 cccaatctta aagggaagag tttacaagat tctgagcgct gaaggggaga gctttctcct
     7621 agccatgtca aggataatct atctagatcc ttctttggga gggatatctg gaatgtccct
     7681 cggaagattc catatacgac agttctcaga ccctgtctct gaagggttat ccttctggag
     7741 agagatctgg ttaagctccc aagagtcctg gattcacgcg ttgtgtcaag aggctggaaa
     7801 cccagatctt ggagagagaa cactcgagag cttcactcgc cttctagaag atccgaccac
     7861 cttaaatatc agaggagggg ccagtcctac cattctactc aaggatgcaa tcagaaaggc
     7921 tttatatgac gaggtggaca aggtggaaaa ttcagagttt cgagaggcaa tcctgttgtc
     7981 caagacccat agagataatt ttatactctt cttaatatct gttgagcctc tgtttcctcg
     8041 atttctcagt gagctattca gttcgtcttt tttgggaatc cccgagtcaa tcattggatt
     8101 gatacaaaac tcccgaacga taagaaggca gtttagaaag agtctctcaa aaactttaga
     8161 agaatccttc tacaactcag agatccacgg gattagtcgg atgacccaga cacctcagag
     8221 ggttgggggg gtgtggcctt gctcttcaga gagggcagat ctacttaggg agatctcttg
     8281 gggaagaaaa gtggtaggca cgacagttcc tcacccttct gagatgttgg gattacttcc
     8341 caagtcctct atttcttgca cttgtggagc aacaggagga ggcaatccta gagtttctgt
     8401 atcagtactc ccgtcctttg atcagtcatt tttttcacga ggccccctaa agggatactt
     8461 gggctcgtcc acctctatgt cgacccagct attccatgca tgggaaaaag tcactaatgt
     8521 tcatgtggtg aagagagctc tatcgttaaa agaatctata aactggttca ttactagaga
     8581 ttccaacttg gctcaagctc taattaggaa cattatgtct ctgacaggcc ctgatttccc
     8641 tctagaggag gcccctgtct tcaaaaggac ggggtcagcc ttgcataggt tcaagtctgc
     8701 cagatacagc gaaggagggt attcttctgt ctgcccgaac ctcctctctc atatttctgt
     8761 tagtacagac accatgtctg atttgaccca agacgggaag aactacgatt tcatgttcca
     8821 gccattgatg ctttatgcac agacatggac atcagagctg gtacagagag acacaaggct
     8881 aagagactct acgtttcatt ggcacctccg atgcaacagg tgtgtgagac ccattgacga
     8941 cgtgaccctg gagacctctc agatcttcga gtttccggat gtgtcgaaaa gaatatccag
     9001 aatggtttct ggggctgtgc ctcacttcca gaggcttccc gatatccgtc tgagaccagg
     9061 agattttgaa tctctaagcg gtagagaaaa gtctcaccat atcggatcag ctcaggggct
     9121 cttatactca atcttagtgg caattcacga ctcaggatac aatgatggaa ccatcttccc
     9181 tgtcaacata tacggcaagg tttcccctag agactatttg agagggctcg caaggggagt
     9241 attgatagga tcctcgattt gcttcttgac aagaatgaca aatatcaata ttaatagacc
     9301 tcttgaattg gtctcagggg taatctcata tattctcctg aggctagata accatccctc
     9361 cttgtacata atgctcagag aaccgtctct tagaggagag atattttcta tccctcagaa
     9421 aatccccgcc gcttatccaa ccactatgaa agaaggcaac agatcaatct tgtgttatct
     9481 ccaacatgtg ctacgctatg agcgagagat aatcacggcg tctccagaga atgactggct
     9541 atggatcttt tcagacttta gaagtgccaa aatgacgtac ctatccctca ttacttacca
     9601 gtctcatctt ctactccaga gggttgagag aaacctatct aagagtatga gagataacct
     9661 gcgacaattg agttctttga tgaggcaggt gctgggcggg cacggagaag ataccttaga
     9721 gtcagacgac aacattcaac gactgctaaa agactcttta cgaaggacaa gatgggtgga
     9781 tcaagaggtg cgccatgcag ctagaaccat gactggagat tacagcccca acaagaaggt
     9841 gtcccgtaag gtaggatgtt cagaatgggt ctgctctgct caacaggttg cagtctctac
     9901 ctcagcaaac ccggcccctg tctcggagct tgacataagg gccctctcta agaggttcca
     9961 gaaccctttg atctcgggct tgagagtggt tcagtgggca accggtgctc attataagct
    10021 taagcctatt ctagatgatc tcaatgtttt cccatctctc tgccttgtag ttggggacgg
    10081 gtcagggggg atatcaaggg cagtcctcaa catgtttcca gatgccaagc ttgtgttcaa
    10141 cagtctttta gaggtgaatg acctgatggc ttccggaaca catccactgc ctccttcagc
    10201 aatcatgagg ggaggaaatg atatcgtctc cagagtgata gatcttgact caatctggga
    10261 aaaaccgtcc gacttgagaa acttggcaac ctggaaatac ttccagtcag tccaaaagca
    10321 ggtcaacatg tcctatgacc tcattatttg cgatgcagaa gttactgaca ttgcatctat
    10381 caaccggatc accctgttaa tgtccgattt tgcattgtct atagatggac cactctattt
    10441 ggtcttcaaa acttatggga ctatgctagt aaatccaaac tacaaggcta ttcaacacct
    10501 gtcaagagcg ttcccctcgg tcacagggtt tatcacccaa gtaacttcgt ctttttcatc
    10561 tgagctctac ctccgattct ccaaacgagg gaagtttttc agagatgctg agtacttgac
    10621 ctcttccacc cttcgagaaa tgagccttgt gttattcaat tgtagcagcc ccaagagtga
    10681 gatgcagaga gctcgttcct tgaactatca ggatcttgtg agaggatttc ctgaagaaat
    10741 catatcaaat ccttacaatg agatgatcat aactctgatt gacagtgatg tagaatcttt
    10801 tctagtccac aagatggttg atgatcttga gttacagagg ggaactctgt ctaaagtggc
    10861 tatcattata gccatcatga tagttttctc caacagagtc ttcaacgttt ccaaacccct
    10921 aactgacccc tcgttctatc caccgtctga tcccaaaatc ctgaggcact tcaacatatg
    10981 ttgcagtact atgatgtatc tatctactgc tttaggtgac gtccctagct tcgcaagact
    11041 tcacgacctg tataacagac ctataactta ttacttcaga aagcaagtca ttcgagggaa
    11101 cgtttatcta tcttggagtt ggtccaacga cacctcagtg ttcaaaaggg tagcctgtaa
    11161 ttctagcctg agtctgtcat ctcactggat caggttgatt tacaagatag tgaagactac
    11221 cagactcgtt ggcagcatca aggatctatc cagagaagtg gaaagacacc ttcataggta
    11281 caacaggtgg atcaccctag aggatatcag atctagatca tccctactag actacagttg
    11341 cctgtgaacc ggatactcct ggaagcctgc ccatgctaag actcttgtgt gatgtatctt
    11401 gaaaaaaaca agatcctaaa tctgaacctt tggttgtttg attgtttttc tcatttttgt
    11461 tgtttatttg ttaagcgtgg gtcggcatgg catctccacc tcctcgcggt ccgacctggg
    11521 catccgaagg aggacgcacg tccactcgga tggctaaggg agaagggcga attccagcac
    11581 actggcggcc gttactagtg gatccgagct cggtaccgag ctcggatcca ctagtccagt
    11641 gtggtggaat tctgcagata tccagcacag tggcggccgc tcgagtctag agggcccgtt
    11701 taaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc
    11761 tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat
    11821 gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg
    11881 caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc
    11941 tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc
    12001 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
    12061 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
    12121 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
    12181 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
    12241 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
    12301 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
    12361 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
    12421 taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca
    12481 gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct
    12541 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc
    12601 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
    12661 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc
    12721 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt
    12781 gtatatccat tttcggatct gatcagcacg tgttgacaat taatcatcgg catagtatat
    12841 cggcatagta taatacgaca aggtgaggaa ctaaaccatg gccaagttga ccagtgccgt
    12901 tccggtgctc accgcgcgcg acgtcgccgg agcggtcgag ttctggaccg accggctcgg
    12961 gttctcccgg gacttcgtgg aggacgactt cgccggtgtg gtccgggacg acgtgaccct
    13021 gttcatcagc gcggtccagg accaggtggt gccggacaac accctggcct gggtgtgggt
    13081 gcgcggcctg gacgagctgt acgccgagtg gtcggaggtc gtgtccacga acttccggga
    13141 cgcctccggg ccggccatga ccgagatcgg cgagcagccg tgggggcggg agttcgccct
    13201 gcgcgacccg gccggcaact gcgtgcactt cgtggccgag gagcaggact gacacgtgct
    13261 acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg
    13321 ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc
    13381 caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
    13441 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
    13501 ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct
    13561 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
    13621 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
    13681 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
    13741 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
    13801 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
    13861 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
    13921 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
    13981 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
    14041 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
    14101 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
    14161 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
    14221 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
    14281 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
    14341 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
    14401 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
    14461 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
    14521 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
    14581 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
    14641 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
    14701 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
    14761 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
    14821 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
    14881 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
    14941 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
    15001 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
    15061 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
    15121 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
    15181 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
    15241 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
    15301 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
    15361 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
    15421 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
    15481 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
    15541 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
    15601 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
    15661 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg acggatcggg
    15721 agatctcccg atcccctatg gtgcactctc agtacaatct gctctgatgc cgcatagtta
    15781 agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt
    15841 taagctacaa caaggcaagg cttgaccgac aattgcatga agaatctgct tagggttagg
    15901 cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc gtt
//
