LOCUS       sequence-137841-         857 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial pMCSG7 expression vector expressing Wildtype Nme cas9 with
            T7 promoter, N-terminal His tag and TEV site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 857)
  AUTHORS   Zhang Y, Rajan R, Seifert HS, Mondragon A, Sontheimer EJ
  TITLE     DNase H Activity of Neisseria meningitidis Cas9.
  JOURNAL   Mol Cell. 2015 Oct 15;60(2):242-55. doi: 
            10.1016/j.molcel.2015.09.020.
  PUBMED    26474066
REFERENCE   2  (bases 1 to 857)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2015.09"; journalName: "Mol Cell"; date: 
            "2015-10-15- 15"; volume: "60"; issue: "2"; pages: "242-55"
COMMENT     Sequence Label: pMCSG7-Wt-NmeCas9
FEATURES             Location/Qualifiers
     source          1..857
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     47..66
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        47..65
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    66..90
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             105..127
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             138..155
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             180..200
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 atgcgtccgg cgtagaggat cgagatcgat ctcgatcccg cgaaattaat acgactcact
       61 ataggggaat tgtgagcgga taacaattcc cctctagaaa taattttgtt taactttaag
      121 aaggagatat acatatgcac catcatcatc atcattcttc tggtgtagat ctgggtaccg
      181 agaacctgta cttccaatcc aatgccattg ctgccttcaa acctaattca atcaactaca
      241 tcctcggcct cgatatcggc atcgcatccg tcggctgggc gatggtagaa attgacgaag
      301 aagaaaaccc catccgcctg attgatttgg gcgtgcgcgt atttgagcgt gccgaagtac
      361 cgaaaacagg cgactccctt gccatggcaa ggcgtttggc gcgcagtgtt cgccgcctga
      421 cccgccgtcg cgcccaccgc ctgcttcgga cccgccgcct attgaaacgc gaaggcgtat
      481 tacaagccgc caattttgac gaaaacggct tgattaaatc cttaccgaat acaccatggc
      541 aacttcgcgc agccgcatta gaccgcaaac tgacgccttt agagtggtcg gcagtcttgt
      601 tgcatttaat caaacatcgc ggctatttat cgcaacggaa aaacgagggc gaaactgccg
      661 ataaggagct tggcgctttg cttaaaggcg tagccggcaa tgcccatgcc ttacagacag
      721 gcgatttccg cacaccggcc gaattggctt taaataaatt tgagaaagaa agcggccata
      781 tccgcaatca gcgcagcgat tattcgcata cgttcagccg caaagattta caggcggagc
      841 tgattttgct gtttgaa
//
