LOCUS       sequence-137882-        7692 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7692)
  AUTHORS   Liao RG, Jung J, Tchaicha JH, Wilkerson MD, Sivachenko A, Beauchamp 
            EM, Liu Q, Pugh TJ, Pedamallu CS, Hayes DN, Gray NS, Getz G, Wong 
            KK, Haddad RI, Meyerson M, Hammerman PS
  TITLE     Inhibitor-sensitive FGFR2 and FGFR3 mutations in lung squamous cell 
            carcinoma.
  JOURNAL   Cancer Res. 2013 Jun 20.
  PUBMED    23786770
REFERENCE   2  (bases 1 to 7692)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer Res.
            2013 Jun 20."
COMMENT     Sequence Label: pBp-FGFR2c-WT
FEATURES             Location/Qualifiers
     source          1..7692
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             8..477
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    542..741
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             934..1350
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1318..1334
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     protein_bind    1378..1402
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    1801..1812
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
     protein_bind    complement(3872..3896)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(3932..3952)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3937..4266
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4117..4252
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4276..4875
                     /codon_start=1
                     /gene="pac from Streptomyces"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVECPKDRATWCMTRKPGA"
     primer_bind     complement(4276..4295)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4772..4792
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             4974..5567
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(5768..5785)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5939..6526)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6019..6038)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6704..7495,7496..7564))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7327..7346
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7565..7669)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctgcagcctg aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca
       61 gggccaagaa cagatggaac agctgaatat gggccaaaca ggatatctgt ggtaagcagt
      121 tcctgccccg gctcagggcc aagaacagat ggtccccaga tgcggtccag ccctcagcag
      181 tttctagaga accatcagat gtttccaggg tgccccaagg acctgaaatg accctgtgcc
      241 ttatttgaac taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga
      301 gctcaataaa agagcccaca acccctcact cggggcgcca gtcctccgat tgactgagtc
      361 gcccgggtac ccgtgtatcc aataaaccct cttgcagttg catccgactt gtggtctcgc
      421 tgttccttgg gagggtctcc tctgagtgat tgactacccg tcagcggggg tctttcattt
      481 gggggctcgt ccgggatcgg gagacccctg cccagggacc accgacccac caccgggagg
      541 taagctggcc agcaacttat ctgtgtctgt ccgattgtct agtgtctatg actgatttta
      601 tgcgcctgcg tcggtactag ttagctaact agctctgtat ctggcggacc cgtggtggaa
      661 ctgacgagtt ctgaacaccc ggccgcaacc ctgggagacg tcccagggac tttgggggcc
      721 gtttttgtgg cccgacctga ggaagggagt cgatgtggaa tccgaccccg tcaggatatg
      781 tggttctggt aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt
      841 cggtttggaa ccgaagccgc gcgtcttgtc tgctgcagca tcgttctgtg ttgtctctgt
      901 ctgactgtgt ttctgtattt gtctgaaaat tagggccaga ctgttaccac tcccttaagt
      961 ttgaccttag atcactggaa agatgtcgag cggctcgctc acaaccagtc ggtagatgtc
     1021 aagaagagac gttgggttac cttctgctct gcagaatggc caacctttaa cgtcggatgg
     1081 ccgcgagacg gcacctttaa ccgagacctc atcacccagg ttaagatcaa ggtcttttca
     1141 cctggcccgc atggacaccc agaccaggtc ccctacatcg tgacctggga agccttggct
     1201 tttgaccccc ctccctgggt caagcccttt gtacacccta agcctccgcc tcctcttctt
     1261 ccatccgcgc cgtctctccc ccttgaacct cctctttcga ccccgcctca atcctccctt
     1321 tatccagccc tcactccttc tctaggcgcc ggccggatcc cagtgtggtg gtacatcaca
     1381 agtttgtaca aaaaagcagg caccatggtc agctggggtc gtttcatctg cctggtcgtg
     1441 gtcaccatgg caaccttgtc cctggcccgg ccctccttca gtttagttga ggataccaca
     1501 ttagagccag aagagccacc aaccaaatac caaatctctc aaccagaagt gtacgtggct
     1561 gcgccagggg agtcgctaga ggtgcgctgc ctgttgaaag atgccgccgt gatcagttgg
     1621 actaaggatg gggtgcactt ggggcccaac aataggacag tgcttattgg ggagtacttg
     1681 cagataaagg gcgccacgcc tagagactcc ggcctctatg cttgtactgc cagtaggact
     1741 gtagacagtg aaacttggta cttcatggtg aatgtcacag atgccatctc atccggagat
     1801 gatgaggatg acaccgatgg tgcggaagat tttgtcagtg agaacagtaa caacaagaga
     1861 gcaccatact ggaccaacac agaaaagatg gaaaagcggc tccatgctgt gcctgcggcc
     1921 aacactgtca agtttcgctg cccagccggg gggaacccaa tgccaaccat gcggtggctg
     1981 aaaaacggga aggagtttaa gcaggagcat cgcattggag gctacaaggt acgaaaccag
     2041 cactggagcc tcattatgga aagtgtggtc ccatctgaca agggaaatta tacctgtgtg
     2101 gtggagaatg aatacgggtc catcaatcac acgtaccacc tggatgttgt ggagcgatcg
     2161 cctcaccggc ccatcctcca agccggactg ccggcaaatg cctccacagt ggtcggagga
     2221 gacgtagagt ttgtctgcaa ggtttacagt gatgcccagc cccacatcca gtggatcaag
     2281 cacgtggaaa agaacggcag taaatacggg cccgacgggc tgccctacct caaggttctc
     2341 aaggccgccg gtgttaacac cacggacaaa gagattgagg ttctctatat tcggaatgta
     2401 acttttgagg acgctgggga atatacgtgc ttggcgggta attctattgg gatatccttt
     2461 cactctgcat ggttgacagt tctgccagcg cctggaagag aaaaggagat tacagcttcc
     2521 ccagactacc tggagatagc catttactgc ataggggtct tcttaatcgc ctgtatggtg
     2581 gtaacagtca tcctgtgccg aatgaagaac acgaccaaga agccagactt cagcagccag
     2641 ccggctgtgc acaagctgac caaacgtatc cccctgcgga gacaggtaac agtttcggct
     2701 gagtccagct cctccatgaa ctccaacacc ccgctggtga ggataacaac acgcctctct
     2761 tcaacggcag acacccccat gctggcaggg gtctccgagt atgaacttcc agaggaccca
     2821 aaatgggagt ttccaagaga taagctgaca ctgggcaagc ccctgggaga aggttgcttt
     2881 gggcaagtgg tcatggcgga agcagtggga attgacaaag acaagcccaa ggaggcggtc
     2941 accgtggccg tgaagatgtt gaaagatgat gccacagaga aagacctttc tgatctggtg
     3001 tcagagatgg agatgatgaa gatgattggg aaacacaaga atatcataaa tcttcttgga
     3061 gcctgcacac aggatgggcc tctctatgtc atagttgagt atgcctctaa aggcaacctc
     3121 cgagaatacc tccgagcccg gaggccaccc gggatggagt actcctatga cattaaccgt
     3181 gttcctgagg agcagatgac cttcaaggac ttggtgtcat gcacctacca gctggccaga
     3241 ggcatggagt acttggcttc ccaaaaatgt attcatcgag atttagcagc cagaaatgtt
     3301 ttggtaacag aaaacaatgt gatgaaaata gcagactttg gactcgccag agatatcaac
     3361 aatatagact attacaaaaa gaccaccaat gggcggcttc cagtcaagtg gatggctcca
     3421 gaagccctgt ttgatagagt atacactcat cagagtgatg tctggtcctt cggggtgtta
     3481 atgtgggaga tcttcacttt agggggctcg ccctacccag ggattcccgt ggaggaactt
     3541 tttaagctgc tgaaggaagg acacagaatg gataagccag ccaactgcac caacgaactg
     3601 tacatgatga tgagggactg ttggcatgca gtgccctccc agagaccaac gttcaagcag
     3661 ttggtagaag acttggatcg aattctcact ctcacaacca atgaggaata cttggacctc
     3721 agccaacctc tcgaacagta ttcacctagt taccctgaca caagaagttc ttgttcttca
     3781 ggagatgatt ctgttttttc tccagacccc atgccttacg aaccatgcct tcctcagtat
     3841 ccacacataa acggcagtgt taaaacatag aacccagctt tcttgtacaa agtggtgatg
     3901 taggaattcg ccagcacagt ggtcgacctg tggaatgtgt gtcagttagg gtgtggaaag
     3961 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     4021 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     4081 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     4141 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     4201 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
     4261 tgcaaaaagc ttaccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac
     4321 gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac
     4381 accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg
     4441 cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc
     4501 tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg
     4561 gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg
     4621 caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag
     4681 ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg
     4741 gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc
     4801 ttcaccgtca ccgccgacgt cgagtgcccg aaggaccgcg cgacctggtg catgacccgc
     4861 aagcccggtg cctgacgccc gccccacgac ccgcagcgcc cgaccgaaag gagcgcacga
     4921 ccccatgcat cgataaaata aaagatttta tttagtctcc agaaaaaggg gggaatgaaa
     4981 gaccccacct gtaggtttgg caagctagct taagtaacgc cattttgcaa ggcatggaaa
     5041 aatacataac tgagaataga gaagttcaga tcaaggtcag gaacagatgg aacagctgaa
     5101 tatgggccaa acaggatatc tgtggtaagc agttcctgcc ccggctcagg gccaagaaca
     5161 gatggaacag ctgaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc
     5221 tcagggccaa gaacagatgg tccccagatg cggtccagcc ctcagcagtt tctagagaac
     5281 catcagatgt ttccagggtg ccccaaggac ctgaaatgac cctgtgcctt atttgaacta
     5341 accaatcagt tcgcttctcg cttctgttcg cgcgcttctg ctccccgagc tcaataaaag
     5401 agcccacaac ccctcactcg gggcgccagt cctccgattg actgagtcgc ccgggtaccc
     5461 gtgtatccaa taaaccctct tgcagttgca tccgacttgt ggtctcgctg ttccttggga
     5521 gggtctcctc tgagtgattg actacccgtc agcgggggtc tttcatttcc gacttgtggt
     5581 ctcgctgcct tgggagggtc tcctctgagt gattgactac ccgtcagcgg gggtcttcac
     5641 atgcagcatg tatcaaaatt aatttggttt tttttcttaa gtatttacat taaatggcca
     5701 tagttgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tggcgctctt
     5761 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     5821 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     5881 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     5941 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     6001 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     6061 ctcctgttcc gaccctgccg cttaccggat accgtccgcc tttctccctt cgggaagcgt
     6121 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     6181 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     6241 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     6301 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     6361 ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     6421 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     6481 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     6541 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     6601 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gtttgcggcc
     6661 gcaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     6721 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     6781 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     6841 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     6901 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     6961 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     7021 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     7081 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     7141 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     7201 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     7261 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     7321 cacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     7381 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     7441 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     7501 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     7561 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     7621 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     7681 gaaaagtgcc ac
//
