LOCUS       sequence-137901-         755 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  construction of TEF2 terminator in HCKan-P receiving vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 755)
  AUTHORS   Guo Y, Dong J, Zhou T, Auxillos J, Li T, Zhang W, Wang L, Shen Y, 
            Luo Y, Zheng Y, Lin J, Chen GQ, Wu Q, Cai Y, Dai J
  TITLE     YeastFab: the design and construction of standard biological parts 
            for metabolic engineering in Saccharomyces cerevisiae.
  JOURNAL   Nucleic Acids Res. 2015 May 8. pii: gkv464.
  PUBMED    25956650
REFERENCE   2  (bases 1 to 755)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 May 8. pii: gkv464."
COMMENT     Sequence Label: HCKan-T-TEF2
FEATURES             Location/Qualifiers
     source          1..755
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      487..518
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     promoter        519..621
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(695..714)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 aggcccaccc gtgaaggtga gccagtgagt tgattgcagt ccagttacgc tggagtccgt
       61 ctcgtagcag taataattat tgcttccata taatattttt atatacctct tatttttatg
      121 tattagttaa ttaagtattt ttatctatct gcttatcatt ttcttttcat ataggggggg
      181 ttggtgtttt cttgcccatc agattgatgt cctccaactc ggcactattt tacaaagggt
      241 ttttttgtaa gagaaggaga agacagatac taaaccatac gttactcgaa acaaaaaaaa
      301 aaaaaatgga aaaagctgct atcaacaaaa gacggcctca tcaaacctaa agaaaccatg
      361 tcagcgtatg tatatacctt gtaatttacg tttccttaaa tcttctttct actaacgttt
      421 tcattattct atactctcct ccgagacgac tgaccattta aatcatacct gacctccata
      481 gcagaaagtc aaaagcctcc gaccggaggc ttttgacttg atcggcacgt aagaggttcc
      541 aactttcacc ataatgaaat aagatcacta ccgggcgtat tttttgagtt atcgagattt
      601 tcaggagcta aggaagctaa aatgagccat attcaacggg aaacgtcttg ctcgaggccg
      661 cgattaaatt ccaacatgga tgctgattta tatgggtata aatgggctcg cgataatgtc
      721 gggcaatcag gtgcgacaat ctatcgattg tatgg
//
