LOCUS       sequence-13792-e        9193 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9193)
  AUTHORS   Chu D, Barnes DJ, von der Haar T
  TITLE     The role of tRNA and ribosome competition in coupling the expression
            of different mRNAs in Saccharomyces cerevisiae.
  JOURNAL   Nucleic Acids Res. 2011 Aug;39(15):6705-14. doi: 10.1093/nar/gkr300.
            Epub 2011 May 9.
  PUBMED    21558172
REFERENCE   2  (bases 1 to 9193)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/nar/gkr300"; journalName: "Nucleic Acids Res"; date: 
            "2011-08"; volume: "39"; issue: "15"; pages: "6705-14"
COMMENT     Sequence Label: pTH650-RLuc/staFLuc
FEATURES             Location/Qualifiers
     source          1..9193
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(10..28)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    70..573
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        610..714
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(715..783,784..1575)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFFHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(933..952)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1746..2334
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2488..2505
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        join(2658..2663,2664..2681,2682..2688)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2696..2712
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2701..2723
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2720..2736
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2720..2736
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        2757..2775
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     terminator      complement(2824..3123)
                     /label=ADH2 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 2 (ADH2) gene"
     CDS             complement(3154..4086)
                     /codon_start=1
                     /product="luciferase from the anthozoan coelenterate 
                     Renilla reniformis (sea pansy)"
                     /label=Rluc
                     /translation="MTSKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     promoter        complement(4094..4490)
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     promoter        4499..5144
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     5036..5059
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
     CDS             5152..6804
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase"
                     /label=luciferase
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     primer_bind     complement(5218..5236)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     terminator      6960..7147
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(7015..7039)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(7209..7228)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7210..7228)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7235..7252)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7235..7251)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7244..7266)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7396..7851
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7483..7502)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7693..7714
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(7980..8783)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(8784..9004)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     primer_bind     complement(9024..9043)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttaggacgg atcgcttgcc tgtaacttac acgcgcctcg tatcttttaa tgatggaata
      121 atttgggaat ttactctgtg tttatttatt tttatgtttt gtatttggat tttagaaagt
      181 aaataaagaa ggtagaagag ttacggaatg aagaaaaaaa aataaacaaa ggtttaaaaa
      241 atttcaacaa aaagcgtact ttacatatat atttattaga caagaaaagc agattaaata
      301 gatatacatt cgattaacga taagtaaaat gtaaaatcac aggattttcg tgtgtggtct
      361 tctacacaga caagatgaaa caattcggca ttaatacctg agagcaggaa gagcaagata
      421 aaaggtagta tttgttggcg atccccctag agtcttttac atcttcggaa aacaaaaact
      481 attttttctt taatttcttt ttttactttc tatttttaat ttatatattt atattaaaaa
      541 atttaaatta taattatttt tatagcacgt gatgaaaagg acccaggtgg cacttttcgg
      601 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
      661 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
      721 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
      781 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
      841 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
      901 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
      961 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     1021 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     1081 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     1141 ccgaaggagc taaccgcttt ttttcacaac atgggggatc atgtaactcg ccttgatcgt
     1201 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     1261 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     1321 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     1381 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     1441 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     1501 ggcagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     1561 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     1621 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     1681 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     1741 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     1801 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     1861 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac
     1921 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     1981 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     2041 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     2101 acgacctaca ccgaactgag atacctacag cgtgagcatt gagaaagcgc cacgcttccc
     2161 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     2221 agggagcttc caggggggaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     2281 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc cgagcctatg gaaaaacgcc
     2341 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     2401 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     2461 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     2521 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
     2581 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttacctcact
     2641 cattaggcac cccaggcttt acactttatg cttccggctc ctatgttgtg tggaattgtg
     2701 agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa gctcggaatt
     2761 aaccctcact aaagggaaca aaagctgggt accgggcccc ccctcgaggg catgcgaagg
     2821 aaaatgagaa atatcgaggg agacgattca gaggagcagg acaaactata accgactgtt
     2881 tgttggagga tgccgtacat aacgaacact gctgaagcta ccatgtctac agtttagagg
     2941 aatgggtaca actcacaggc gagggatggt gttcactcgt gctagcaaac gcggtgggag
     3001 caaaaagtag aatattatct tttattcgtg aaacttcgaa cactgtcatc taaagatgct
     3061 atatactaat ataggcatac ttgataatgg aaactataaa tcgtgaaggc ataggagatc
     3121 cgcttattta gaagtgtcga attcttacga catttgttca tttttgagaa ctcgctcaac
     3181 gaacgatttg atatattttc ccatttcatc aggtgcatct tcttgcgaaa aatgaagacc
     3241 ttttactttg acaaattcag tattaggaaa cttcttggca ccttcaacaa tagcattgga
     3301 aaagaatcct gggtccgatt caataaacat ttttggtaaa tcatcacttg cacgtagata
     3361 agcattataa ttcctaacaa tttgtacaac gtcaggttta ccacctttta ctaacgggat
     3421 ttcacgaggc catgataatg ttggacgacg aacttcacct ttctctttga atggttcaag
     3481 atatgctgca aattcttctg gttctaactt tctcatgatt tttgatggca acatggtttc
     3541 cacgaagaag ttattctcca aaaccatttt ttctccttct tcagatttga tcaacgcaat
     3601 atcttcttca atatcaggcc attcatccca tgattcaatc acatctacta cactttcagc
     3661 gtgaactatt gctttgatct tatcttgatg ctcatagcta taatgaaatg ccaaacaagc
     3721 accccaatca tggccgacaa aaatgatctt ctttggtaaa ttaagaagtt caaaccatgc
     3781 agtaagatat ttgtaatgat caagtaacct ataagaacca ttaccagatt tgcctgattt
     3841 gcccatacca ataaggtctg gtataataca ccgcgctact ggctcaatat gtggcacaac
     3901 atgtcgccat aaataagaag aggccgcgtt accatgtaaa aaaataacag cattttctgc
     3961 atgtttttct gaatcataat aattaataaa tgaatcaaga acattcattt gtttacatct
     4021 ggcccaccac tgcggaccag ttatcatccg tttcctttgt tctggatcat aaactttcga
     4081 agtcatcccg gggagttgat tgtatgcttg gtatagcttg aaatattgtg cagaaaaaga
     4141 aacaaggaag aaagggaacg agaacaatga cgaggaaaca aaagattaat aattgcaggt
     4201 ctatttatac ttgatagcaa agcggcaaac tttttttatt tcaaattcaa gtaactggaa
     4261 ggaaggccgt ataccgttgc tcattagaga gtagtgtgcg tgaatgaagg aaggaaaaag
     4321 tttcgtgtgt tcgaagatac ccctcatcag ctctggaaca acgacatctg ttggtgctgt
     4381 ctttgtcgtt aattttttcc tttagtgtct tccatcattt tttttgtcat tgcggatatg
     4441 gtgagacaac aacgggggag agagaaaaga aaaaaaaaga aaagaatccg ggagtttatc
     4501 attatcaata ctgccatttc aaagaatacg taaataatta atagtagtga ttttcctaac
     4561 tttatttagt caaaaaatta gccttttaat tctgctgtaa cccgtacatg cccaaaatag
     4621 ggggcgggtt acacagaata tataacatcg taggtgtctg ggtgaacagt ttattcctgg
     4681 catccactaa atataatgga gcccgctttt taagctggca tccagaaaaa aaaagaatcc
     4741 cagcaccaaa atattgtttt cttcaccaac catcagttca taggtccatt ctcttagcgc
     4801 aactacagag aacaggggca caaacaggca aaaaacgggc acaacctcaa tggagtgatg
     4861 caacctgcct ggagtaaatg atgacacaag gcaattgacc cacgcatgta tctatctcat
     4921 tttcttacac cttctattac cttctgctct ctctgatttg gaaaaagctg aaaaaaaagg
     4981 ttgaaaccag ttccctgaaa ttattcccct acttgactaa taagtatata aagaacggta
     5041 ggtattgatt gtaattctgt aaatctattt cttaaacttc ttaaattcta cttttatagt
     5101 tagtcttttt ttttagtttt aaaacaccaa gaacttagtt tcgacggatc catggaagac
     5161 gccaaaaaca taaagaaagg cccggcgcca ttctatcctc tagaggatgg aaccgctgga
     5221 gagcaactgc ataaggctat gaagagatac gccctggttc ctggaacaat tgcttttaca
     5281 gatgcacata tcgaggtgaa catcacgtac gcggaatact tcgaaatgtc cgttcggttg
     5341 gcagaagcta tgaaacgata tgggctgaat acaaatcaca gaatcgtcgt atgcagtgaa
     5401 aactctcttc aattctttat gccggtgttg ggcgcgttat ttatcggagt tgcagttgcg
     5461 cccgcgaacg acatttataa tgaacgtgaa ttgctcaaca gtatgaacat ttcgcagcct
     5521 accgtagtgt ttgtttccaa aaaggggttg caaaaaattt tgaacgtgca aaaaaaatta
     5581 ccaataatcc agaaaattat tatcatggat tctaaaacgg attaccaggg atttcagtcg
     5641 atgtacacgt tcgtcacatc tcatctacct cccggtttta atgaatacga ttttgtacca
     5701 gagtcctttg atcgtgacaa aacaattgca ctgataatga attcctctgg atctactggg
     5761 ttacctaagg gtgtggccct tccgcataga actgcctgcg tcagattctc gcatgccaga
     5821 gatcctattt ttggcaatca aatcattccg gatactgcga ttttaagtgt tgttccattc
     5881 catcacggtt ttggaatgtt tactacactc ggatatttga tatgtggatt tcgagtcgtc
     5941 ttaatgtata gatttgaaga agagctgttt ttacgatccc ttcaggatta caaaattcaa
     6001 agtgcgttgc tagtaccaac cctattttca ttcttcgcca aaagcactct gattgacaaa
     6061 tacgatttat ctaatttaca cgaaattgct tctgggggcg cacctctttc gaaagaagtc
     6121 ggggaagcgg ttgcaaaacg cttccatctt ccagggatac gacaaggata tgggctcact
     6181 gagactacat cagctattct gattacaccc gagggggatg ataaaccggg cgcggtcggt
     6241 aaagttgttc cattttttga agcgaaggtt gtggatctgg ataccgggaa aacgctgggc
     6301 gttaatcaga gaggcgaatt atgtgtcaga ggacctatga ttatgtccgg ttatgtaaac
     6361 aatccggaag cgaccaacgc cttgattgac aaggatggat ggctacattc tggagacata
     6421 gcttactggg acgaagacga acacttcttc atagttgacc gcttgaagtc tttaattaaa
     6481 tacaaaggat atcaggtggc ccccgctgaa ttggaatcga tattgttaca acaccccaac
     6541 atcttcgacg cgggcgtggc aggtcttccc gacgatgacg ccggtgaact tcccgccgcc
     6601 gttgttgttt tggagcacgg aaagacgatg acggaaaaag agatcgtgga ttacgtcgcc
     6661 agtcaagtaa caaccgcgaa aaagttgcgc ggaggagttg tgtttgtgga cgaagtaccg
     6721 aaaggtctta ccggaaaact cgacgcaaga aaaatcagag agatcctcat aaaggccaag
     6781 aagggcggaa agtccaaatt gtaagtcgac ctgcaggcat gcaagctttg gacttcttcg
     6841 ccagaggttt ggtcaagtct ccaatcaagg ttgtcggctt gtctaccttg ccagaaattt
     6901 acgaaaagat ggaaaagggt caaatcgttg gtagatacgt tgttgacact tctaaataag
     6961 cgaatttctt atgatttatg atttttatta ttaaataagt tataaaaaaa ataagtgtat
     7021 acaaatttta aagtgactct taggttttaa aacgaaaatt cttgttcttg agtaactctt
     7081 tcctgtaggt caggttgctt tctcaggtat agcatgaggt cgctcttatt gaccacacct
     7141 ctaccggcat gcccgaaatt cccctaccct atgaaaagcg gccgccaccg cggtggagct
     7201 ccaattcgcc ctatagtgag tcgtattaca attcactggc cgtcgtttta caacgtcgtg
     7261 actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc cccttcgcca
     7321 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga
     7381 atggcgaatg gcgcgacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     7441 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
     7501 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
     7561 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
     7621 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
     7681 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
     7741 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
     7801 tttaacaaaa atttaacgcg aattttaaca aaatattaac gtttacaatt tcctgatgcg
     7861 gtattttctc cttacgcatc tgtgcggtat ttcacaccgc agggtaataa ctgatataat
     7921 taaattgaag ctctaatttg tgagtttagt atacatgcat ttacttataa tacagttttt
     7981 tagttttgct ggccgcatct tctcaaatat gcttcccagc ctgcttttct gtaacgttca
     8041 ccctctacct tagcatccct tccctttgca aatagtcctc ttccaacaat aataatgtca
     8101 gatcctgtag agaccacatc atccacggtt ctatactgtt gacccaatgc gtctcccttg
     8161 tcatctaaac ccacaccggg tgtcataatc aaccaatcgt aaccttcatc tcttccaccc
     8221 atgtctcttt gagcaataaa gccgataaca aaatctttgt cgctcttcgc aatgtcaaca
     8281 gtacccttag tatattctcc agtagatagg gagcccttgc atgacaattc tgctaacatc
     8341 aaaaggcctc taggttcctt tgttacttct tctgccgcct gcttcaaacc gctaacaata
     8401 cctgggccca ccacaccgtg tgcattcgta atgtctgccc attctgctat tctgtataca
     8461 cccgcagagt actgcaattt gactgtatta ccaatgtcag caaattttct gtcttcgaag
     8521 agtaaaaaat tgtacttggc ggataatgcc tttagcggct taactgtgcc ctccatggaa
     8581 aaatcagtca agatatccac atgtgttttt agtaaacaaa ttttgggacc taatgcttca
     8641 actaactcca gtaattcctt ggtggtacga acatccaatg aagcacacaa gtttgtttgc
     8701 ttttcgtgca tgatattaaa tagcttggca gcaacaggac taggatgagt agcagcacgt
     8761 tccttatatg tagctttcga catgatttat cttcgtttcc tgcaggtttt tgttctgtgc
     8821 agttgggtta agaatactgg gcaatttcat gtttcttcaa cactacatat gcgtatatat
     8881 accaatctaa gtctgtgctc cttccttcgt tcttccttct gttcggagat taccgaatca
     8941 aaaaaatttc aaagaaaccg aaatcaaaaa aaagaataaa aaaaaaatga tgaattgaat
     9001 tgaaaagcgt ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc
     9061 cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct
     9121 tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca
     9181 ccgaaacgcg cga
//
