LOCUS       sequence-137930-        9980 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  high copy number plasmid as receiving vector for exogenous pathway.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9980)
  AUTHORS   Guo Y, Dong J, Zhou T, Auxillos J, Li T, Zhang W, Wang L, Shen Y, 
            Luo Y, Zheng Y, Lin J, Chen GQ, Wu Q, Cai Y, Dai J
  TITLE     YeastFab: the design and construction of standard biological parts 
            for metabolic engineering in Saccharomyces cerevisiae.
  JOURNAL   Nucleic Acids Res. 2015 May 8. pii: gkv464.
  PUBMED    25956650
REFERENCE   2  (bases 1 to 9980)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 May 8. pii: gkv464."
COMMENT     Sequence Label: pRS424-URR
FEATURES             Location/Qualifiers
     source          1..9980
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    515..536
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     protein_bind    637..658
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(673..678,679..696,697..703)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    711..727
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             745..756
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             763..1440
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     terminator      1485..1556
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1572..1599
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     misc_feature    1606..1626
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     promoter        1915..2322
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             2323..3417
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKVDAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVDPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     primer_bind     complement(4425..4441)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4425..4441)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(4478..4498)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(4478..4496)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4517..4533)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4517..4533)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    4541..4557
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4565..4571,4572..4589,4590..4595))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     primer_bind     complement(4748..4765)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4919..5507)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4999..5018)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5678..6469,6470..6538))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6301..6320
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6539..6643)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      6670..8012
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(7633..7680)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     8053..8071
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8109..8131)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8231..8250
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        8267..8547
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     CDS             8548..9222
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     rep_origin      complement(9321..9776)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9458..9479)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9670..9689
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9906..9928
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9920..9937
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9921..9937
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9947..9966
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        9947..9965
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ggccctctaa gcacagtcgc gcgtccgaac ctagctctac tttagaggcc ccggattctg
       61 gtgctcgtag accgcagaac cgattggggg gatgtacaac aatatttgtt agtcaccttt
      121 gggtcacgat ctcccacctt actggaattt agtccctgct ataatttgcc ttgcatataa
      181 gttgcgttac ttcagcgtcc taaccgcacc cttagcacga agacagattt gttcattccc
      241 atactccggc gttggcaggg ggttcgcatg tcccacgtga aacgttgcta aaccctcagg
      301 tttctgagcg acaaaagctt taaacgggag ttcgcgctca taacttggtc cgaatgcggg
      361 ttcttgcatc gttcgactga gtttgtttca tgtagaacgg gcgcaaagta tacttagttc
      421 aatcttcaat acctcgtatc attgtacacc tgccggtcac cacccaacga tgtggggacg
      481 gcgttgcaac ttcgaggacc tcgagacccc attggaattc gcggccgctt ctagagcaat
      541 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      601 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      661 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      721 ataacaattt cacacatact agagaaagag gagaaatact agatggcttc ctccgaagac
      781 gttatcaaag agttcatgcg tttcaaagtt cgtatggaag gttccgttaa cggtcacgag
      841 ttcgaaatcg aaggtgaagg tgaaggtcgt ccgtacgaag gtacccagac cgctaaactg
      901 aaagttacca aaggtggtcc gctgccgttc gcttgggaca tcctgtcccc gcagttccag
      961 tacggttcca aagcttacgt taaacacccg gctgacatcc cggactacct gaaactgtcc
     1021 ttcccggaag gtttcaaatg ggaacgtgtt atgaacttcg aagacggtgg tgttgttacc
     1081 gttacccagg actcctccct gcaagacggt gagttcatct acaaagttaa actgcgtggt
     1141 accaacttcc cgtccgacgg tccggttatg cagaaaaaaa ccatgggttg ggaagcttcc
     1201 accgaacgta tgtacccgga agacggtgct ctgaaaggtg aaatcaaaat gcgtctgaaa
     1261 ctgaaagacg gtggtcacta cgacgctgaa gttaaaacca cctacatggc taaaaaaccg
     1321 gttcagctgc cgggtgctta caaaaccgac atcaaactgg acatcacctc ccacaacgaa
     1381 gactacacca tcgttgaaca gtacgaacgt gctgaaggtc gtcactccac cggtgcttaa
     1441 taacgctgat agtgctagtg tagatcgcta ctagagccag gcatcaaata aaacgaaagg
     1501 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact
     1561 agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttatatacta gtagcggccg
     1621 ctgcagtcag acggtctcat gagctgtgcg gtatttcaca ccgcatatcg acggtcgagg
     1681 agaacttcta gtatatccac atacctaata ttattgcctt attaaaaatg gaatcccaac
     1741 aattacatca aaatccacat tctcttcaaa atcaattgtc ctgtacttcc ttgttcatgt
     1801 gtgttcaaaa acgttatatt tataggataa ttatactcta tttctcaaca agtaattggt
     1861 tgtttggccg agcggtctaa ggcgcctgat tcaagaaata tcttgaccgc agttaactgt
     1921 gggaatactc aggtatcgta agatgcaaga gttcgaatct cttagcaacc attatttttt
     1981 tcctcaacat aacgagaaca cacaggggcg ctatcgcaca gaatcaaatt cgatgactgg
     2041 aaattttttg ttaatttcag aggtcgcctg acgcatatac ctttttcaac tgaaaaattg
     2101 ggagaaaaag gaaaggtgag agcgccggaa ccggcttttc atatagaata gagaagcgtt
     2161 catgactaaa tgcttgcatc acaatacttg aagttgacaa tattatttaa ggacctattg
     2221 ttttttccaa taggtggtta gcaatcgtct tactttctaa cttttcttac cttttacatt
     2281 tcagcaatat atatatatat atttcaagga tataccattc taatgtctgc ccctaagaag
     2341 atcgtcgttt tgccaggtga ccacgttggt caagaaatca cagccgaagc cattaaggtt
     2401 cttaaagcta tttctgatgt tcgttccaat gtcaagttcg atttcgaaaa tcatttaatt
     2461 ggtggtgctg ctatcgatgc tacaggtgtt ccacttccag atgaggcgct ggaagcctcc
     2521 aagaaggttg atgccgtttt gttaggtgct gtgggtggtc ctaaatgggg taccggtagt
     2581 gttagacctg aacaaggttt actaaaaatc cgtaaagaac ttcaattgta cgccaactta
     2641 agaccatgta actttgcatc cgactctctt ttagacttat ctccaatcaa gccacaattt
     2701 gctaaaggta ctgacttcgt tgttgtcaga gaattagtgg gaggtattta ctttggtaag
     2761 agaaaggaag acgatggtga tggtgtcgct tgggatagtg aacaatacac cgttccagaa
     2821 gtgcaaagaa tcacaagaat ggccgctttc atggccctac aacatgagcc accattgcct
     2881 atttggtcct tggataaagc taatgttttg gcctcttcaa gattatggag aaaaactgtg
     2941 gaggaaacca tcaagaacga attccctaca ttgaaggttc aacatcaatt gattgattct
     3001 gccgccatga tcctagttaa gaacccaacc cacctaaatg gtattataat caccagcaac
     3061 atgtttggtg atatcatctc cgatgaagcc tccgttatcc caggttcctt gggtttgttg
     3121 ccatctgcgt ccttggcctc tttgccagac aagaacaccg catttggttt gtacgaacca
     3181 tgccacggtt ctgctccaga tttgccaaag aataaggttg accctatcgc cactatcttg
     3241 tctgctgcaa tgatgttgaa attgtcattg aacttgcctg aagaaggtaa ggccattgaa
     3301 gatgcagtta aaaaggtttt ggatgcaggt atcagaactg gtgatttagg tggttccaac
     3361 agtaccaccg aagtcggtga tgctgtcgcc gaagaagtta agaaaatcct tgcttaaaaa
     3421 gattctcttt ttttatgata tttgtacata aactttataa atgaaattca taatagaaac
     3481 gacacgaaat tacaaaatgg aatatgttca tagggtagac gaaactatat acgcaatcta
     3541 catacattta tcaagaagga gaaaaaggag gatagtaaag gaatacaggt aagcaaattg
     3601 atactaatgg ctcaacgtga taaggaaaaa gaattgcact ttaacattaa tattgacaag
     3661 gaggagggca ccacacaaaa agttaggtgt aacagaaaat catgaaacta cgattcctaa
     3721 tttgatattg gaggattttc tctaaaaaaa aaaaaataca acaaataaaa aacactcaat
     3781 gacctgacca tttgatggag tttaagtcaa taccttcttg aaccatttcc cataatggtg
     3841 aaagttccct caagaatttt actctgtcag aaacggcctt acgacgtagt cgatatggtg
     3901 cactctcagt acaatctcgc tagtaacatc agctaacgaa agagttagag gctcgctaaa
     3961 tcgcactgtc ggggtccctt gggtatttta cactagcgtc aggacgacta gcatgtgtct
     4021 ttccttccag gggtatgcgg gtgcgtggac aaatgagcag catacgtatt tactcggcgt
     4081 gcctgctctc tcgtatttct cctggagatc aaggaaatgt ttcatgtcca agcgaaaagc
     4141 cgctctacgg aatggatcta cgttactgcc tgcataagga aaccggtgta gccaaggacg
     4201 aaagcgaccc taggttctaa ccatcgactt tggcggaaag gtttcactca ggaagcagac
     4261 actgattgac acggtttagc agaacgtttg aggactaggt caaattgagt ggtttaatat
     4321 cggcatgtct ggctttaaaa ttcagtatag tgcgctgatc ggaaacgaat taaaaacacg
     4381 agttcccaaa accaggcggg ctcgccacgc taatcggccc gggggatcca ctagttctag
     4441 agcggccgcc accgcggtgg agctccagct tttgttccct ttagtgaggg ttaattgcgc
     4501 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     4561 cacacaacat aggagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgaggt
     4621 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     4681 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     4741 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     4801 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     4861 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     4921 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     4981 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     5041 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     5101 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     5161 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     5221 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     5281 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     5341 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct
     5401 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     5461 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     5521 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     5581 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     5641 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     5701 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     5761 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     5821 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     5881 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     5941 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     6001 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     6061 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     6121 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     6181 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     6241 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     6301 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     6361 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     6421 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     6481 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     6541 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     6601 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     6661 tgccacctga acgaagcatc tgtgcttcat tttgtagaac aaaaatgcaa cgcgagagcg
     6721 ctaatttttc aaacaaagaa tctgagctgc atttttacag aacagaaatg caacgcgaaa
     6781 gcgctatttt accaacgaag aatctgtgct tcatttttgt aaaacaaaaa tgcaacgcga
     6841 gagcgctaat ttttcaaaca aagaatctga gctgcatttt tacagaacag aaatgcaacg
     6901 cgagagcgct attttaccaa caaagaatct atacttcttt tttgttctac aaaaatgcat
     6961 cccgagagcg ctatttttct aacaaagcat cttagattac tttttttctc ctttgtgcgc
     7021 tctataatgc agtctcttga taactttttg cactgtaggt ccgttaaggt tagaagaagg
     7081 ctactttggt gtctattttc tcttccataa aaaaagcctg actccacttc ccgcgtttac
     7141 tgattactag cgaagctgcg ggtgcatttt ttcaagataa aggcatcccc gattatattc
     7201 tataccgatg tggattgcgc atactttgtg aacagaaagt gatagcgttg atgattcttc
     7261 attggtcaga aaattatgaa cggtttcttc tattttgtct ctatatacta cgtataggaa
     7321 atgtttacat tttcgtattg ttttcgattc actctatgaa tagttcttac tacaattttt
     7381 ttgtctaaag agtaatacta gagataaaca taaaaaatgt agaggtcgag tttagatgca
     7441 agttcaagga gcgaaaggtg gatgggtagg ttatataggg atatagcaca gagatatata
     7501 gcaaagagat acttttgagc aatgtttgtg gaagcggtat tcgcaatatt ttagtagctc
     7561 gttacagtcc ggtgcgtttt tggttttttg aaagtgcgtc ttcagagcgc ttttggtttt
     7621 caaaagcgct ctgaagttcc tatactttct agagaatagg aacttcggaa taggaacttc
     7681 aaagcgtttc cgaaaacgag cgcttccgaa aatgcaacgc gagctgcgca catacagctc
     7741 actgttcacg tcgcacctat atctgcgtgt tgcctgtata tatatataca tgagaagaac
     7801 ggcatagtgc gtgtttatgc ttaaatgcgt acttatatgc gtctatttat gtaggatgaa
     7861 aggtagtcta gtacctcctg tgatattatc ccattccatg cggggtatcg tatgcttcct
     7921 tcagcactac cctttagctg ttctatatgc tgccactcct caattggatt agtctcatcc
     7981 ttcaatgcta tcatttcctt tgatattgga tcatattaag aaaccattat tatcatgaca
     8041 ttaacctata aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac
     8101 ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat
     8161 gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg
     8221 cttaactatg cggcatcaga gcagattgta ctgagagtgc accataaacg acattactat
     8281 atatataata taggaagcat ttaatagaca gcatcgtaat atatgtgtac tttgcagtta
     8341 tgacgccaga tggcagtagt ggaagatatt ctttattgaa aaatagcttg tcaccttacg
     8401 tacaatcttg atccggagct tttctttttt tgccgattaa gaattaattc ggtcgaaaaa
     8461 agaaaaggag agggccaaga gggagggcat tggtgactat tgagcacgtg agtatacgtg
     8521 attaagcaca caaaggcagc ttggagtatg tctgttatta atttcacagg tagttctggt
     8581 ccattggtga aagtttgcgg cttgcagagc acagaggccg cagaatgtgc tctagattcc
     8641 gatgctgact tgctgggtat tatatgtgtg cccaatagaa agagaacaat tgacccggtt
     8701 attgcaagga aaatttcaag tcttgtaaaa gcatataaaa atagttcagg cactccgaaa
     8761 tacttggttg gcgtgtttcg taatcaacct aaggaggatg ttttggctct ggtcaatgat
     8821 tacggcattg atatcgtcca actgcatgga gatgagtcgt ggcaagaata ccaagagttc
     8881 ctcggtttgc cagttattaa aagactcgta tttccaaaag actgcaacat actactcagt
     8941 gcagcttcac agaaacctca ttcgtttatt cccttgtttg attcagaagc aggtgggaca
     9001 ggtgaacttt tggattggaa ctcgatttct gactgggttg gaaggcaaga gagccccgaa
     9061 agcttacatt ttatgttagc tggtggactg acgccagaaa atgttggtga tgcgcttaga
     9121 ttaaatggcg ttattggtgt tgatgtaagc ggaggtgtgg agacaaatgg tgtaaaagac
     9181 tctaacaaaa tagcaaattt cgtcaaaaat gctaagaaat aggttattac tgagtagtat
     9241 ttatttaagt attgtttgtg cacttgccta tgcggtgtga aataccgcac agatgcgtaa
     9301 ggagaaaata ccgcatcagg aaattgtaaa cgttaatatt ttgttaaaat tcgcgttaaa
     9361 tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa
     9421 atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact
     9481 attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc
     9541 actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa
     9601 tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc
     9661 gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt
     9721 cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtcgcg
     9781 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
     9841 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
     9901 gttttcccag tcacgacgtt gtaaaacgac ggccagtgag cgcgcgtaat acgactcact
     9961 atagggcgaa ttgggtaccg
//
