LOCUS       sequence-137947-         747 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  construction of TDH3 promoter in HCKan-P receiving vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 747)
  AUTHORS   Guo Y, Dong J, Zhou T, Auxillos J, Li T, Zhang W, Wang L, Shen Y, 
            Luo Y, Zheng Y, Lin J, Chen GQ, Wu Q, Cai Y, Dai J
  TITLE     YeastFab: the design and construction of standard biological parts 
            for metabolic engineering in Saccharomyces cerevisiae.
  JOURNAL   Nucleic Acids Res. 2015 May 8. pii: gkv464.
  PUBMED    25956650
REFERENCE   2  (bases 1 to 747)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 May 8. pii: gkv464."
COMMENT     Sequence Label: HCKan-P-TDH3
FEATURES             Location/Qualifiers
     source          1..747
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(28..130)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     terminator      complement(131..162)
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     promoter        complement(212..499)
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     complement(319..342)
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
ORIGIN
        1 agacgtttcc cgttgaatat ggctcatttt agcttcctta gctcctgaaa atctcgataa
       61 ctcaaaaaat acgcccggta gtgatcttat ttcattatgg tgaaagttgg aacctcttac
      121 gtgccgatca agtcaaaagc ctccggtcgg aggcttttga ctttctgcta tggaggtcag
      181 gtatgattta aatggtcagt cgtctcgcat ctttgtttgt ttatgtgtgt ttattcgaaa
      241 ctaagttctt ggtgttttaa aactaaaaaa aagactaact ataaaagtag aatttaagaa
      301 gtttaagaaa tagatttaca gaattacaat caatacctac cgtctttata tacttattag
      361 tcaagtaggg gaataatttc agggaactgg tttcaacctt ttttttcagc tttttccaaa
      421 tcagagagag cagaaggtaa tagaaggtgt aagaaaatga gatagataca tgcgtgggtc
      481 aattgccttg tgtcatcatt tactccaggc aggttgcatc actccattga ggttgtgccc
      541 gttttttgcc tgtttgtgcc cctgttctct gtagttgcgc taagagaatg gacctatgaa
      601 ctgatggttg gtgaagaaaa caatattttg gtgctgggat tctttttttt tctggatgcc
      661 agcttaaaaa gcgggctcca ttatatttag tggatgccag gaataaactg ttcacccaga
      721 cacctacgat gttatatatt ctgtgta
//
