LOCUS       sequence-137948-         706 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial pMCSG7 expression vector expressing Nme cas9 nickase with 
            H588A mutation (HNH domain) with T7 promoter, N-terminal His tag and
            TEV site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 706)
  AUTHORS   Zhang Y, Rajan R, Seifert HS, Mondragon A, Sontheimer EJ
  TITLE     DNase H Activity of Neisseria meningitidis Cas9.
  JOURNAL   Mol Cell. 2015 Oct 15;60(2):242-55. doi: 
            10.1016/j.molcel.2015.09.020.
  PUBMED    26474066
REFERENCE   2  (bases 1 to 706)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2015.09"; journalName: "Mol Cell"; date: 
            "2015-10-15- 15"; volume: "60"; issue: "2"; pages: "242-55"
COMMENT     Sequence Label: pMCSG7-H588A-NmeCas9
FEATURES             Location/Qualifiers
     source          1..706
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    16..40
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             55..77
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             88..105
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             130..150
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 acgactcact ataggggaat tgtgagcgga taacaattcc cctctagaaa taattttgtt
       61 taactttaag aaggagatat acatatgcac catcatcatc atcattcttc tggtgtagat
      121 ctgggtaccg agaacctgta cttccaatcc aatgccattg ctgccttcaa acctaattca
      181 atcaactaca tcctcggcct cgatatcggc atcgcatccg tcggctgggc gatggtagaa
      241 attgacgaag aagaaaaccc catccgcctg attgatttgg gcgtgcgcgt atttgagcgt
      301 gccgaagtac cgaaaacagg cgactccctt gccatggcaa ggcgtttggc gcgcagtgtt
      361 cgccgcctga cccgccgtcg cgcccaccgc ctgcttcgga cccgccgcct attgaaacgc
      421 gaaggcgtat tacaagccgc caattttgac gaaaacggct tgattaaatc cttaccgaat
      481 acaccatggc aacttcgcgc agccgcatta gaccgcaaac tgacgccttt agagtggtcg
      541 gcagtcttgt tgcatttaat caaacatcgc ggctatttat cgcaacggaa aaacgagggc
      601 gaaactgccg ataaggagct tggcgctttg cttaaaggcg tagccggcaa tgcccatgcc
      661 ttacagacag gcgatttccg cacaccggcc gaattggctt taaata
//
