LOCUS       sequence-137949-         688 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  construction of TEF2 terminator in HCKan-P receiving vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 688)
  AUTHORS   Guo Y, Dong J, Zhou T, Auxillos J, Li T, Zhang W, Wang L, Shen Y, 
            Luo Y, Zheng Y, Lin J, Chen GQ, Wu Q, Cai Y, Dai J
  TITLE     YeastFab: the design and construction of standard biological parts 
            for metabolic engineering in Saccharomyces cerevisiae.
  JOURNAL   Nucleic Acids Res. 2015 May 8. pii: gkv464.
  PUBMED    25956650
REFERENCE   2  (bases 1 to 688)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 May 8. pii: gkv464."
COMMENT     Sequence Label: HCKan-T-TEF2
FEATURES             Location/Qualifiers
     source          1..688
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(28..130)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     terminator      complement(131..162)
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     terminator      626..655
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
ORIGIN
        1 agacgtttcc cgttgaatat ggctcatttt agcttcctta gctcctgaaa atctcgataa
       61 ctcaaaaaat acgcccggta gtgatcttat ttcattatgg tgaaagttgg aacctcttac
      121 gtgccgatca agtcaaaagc ctccggtcgg aggcttttga ctttctgcta tggaggtcag
      181 gtatgattta aatggtcagt cgtctcggag gagagtatag aataatgaaa acgttagtag
      241 aaagaagatt taaggaaacg taaattacaa ggtatataca tacgctgaca tggtttcttt
      301 aggtttgatg aggccgtctt ttgttgatag cagctttttc catttttttt ttttttgttt
      361 cgagtaacgt atggtttagt atctgtcttc tccttctctt acaaaaaaac cctttgtaaa
      421 atagtgccga gttggaggac atcaatctga tgggcaagaa aacaccaacc ccccctatat
      481 gaaaagaaaa tgataagcag atagataaaa atacttaatt aactaataca taaaaataag
      541 aggtatataa aaatattata tggaagcaat aattattact gctacgagac ggactccagc
      601 gtaactggac tgcaatcaac tcactggctc accttcacgg gtgggccttt cttcggtaga
      661 aaatcaaagg atcttcttga gatccttt
//
