LOCUS       sequence-137952-         245 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial pMCSG7 expression vector expressing Nme cas9 nickase with 
            H588A mutation (HNH domain) with T7 promoter, N-terminal His tag and
            TEV site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 245)
  AUTHORS   Zhang Y, Rajan R, Seifert HS, Mondragon A, Sontheimer EJ
  TITLE     DNase H Activity of Neisseria meningitidis Cas9.
  JOURNAL   Mol Cell. 2015 Oct 15;60(2):242-55. doi: 
            10.1016/j.molcel.2015.09.020.
  PUBMED    26474066
REFERENCE   2  (bases 1 to 245)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2015.09"; journalName: "Mol Cell"; date: 
            "2015-10-15- 15"; volume: "60"; issue: "2"; pages: "242-55"
COMMENT     Sequence Label: pMCSG7-H588A-NmeCas9
FEATURES             Location/Qualifiers
     source          1..245
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 attggtaatt tgtccgttgg atgcaaagac acgtttcttg cctttacctg tcagccgcat
       61 acggtcggca acaaattgac acaggaaacg gttgacgtag cgcgtgtcgt tcagattgcg
      121 ttctttaaag ccgtcttcat cgaatttttg cagcagaatc cgttgttttt tactgcgcgg
      181 gaaacggctg gtttcgacac gcgctttaaa ttcctgccat tcgcggctgt tgtctttgcc
      241 gttga
//
