LOCUS       sequence-137953-         704 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  construction of ycrtYB gene in HCKan-O receiving vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 704)
  AUTHORS   Guo Y, Dong J, Zhou T, Auxillos J, Li T, Zhang W, Wang L, Shen Y, 
            Luo Y, Zheng Y, Lin J, Chen GQ, Wu Q, Cai Y, Dai J
  TITLE     YeastFab: the design and construction of standard biological parts 
            for metabolic engineering in Saccharomyces cerevisiae.
  JOURNAL   Nucleic Acids Res. 2015 May 8. pii: gkv464.
  PUBMED    25956650
REFERENCE   2  (bases 1 to 704)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 May 8. pii: gkv464."
COMMENT     Sequence Label: HCKan-O-ycrtYB
FEATURES             Location/Qualifiers
     source          1..704
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(28..130)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     terminator      complement(131..162)
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
ORIGIN
        1 agacgtttcc cgttgaatat ggctcatttt agcttcctta gctcctgaaa atctcgataa
       61 ctcaaaaaat acgcccggta gtgatcttat ttcattatgg tgaaagttgg aacctcttac
      121 gtgccgatca agtcaaaagc ctccggtcgg aggcttttga ctttctgcta tggaggtcag
      181 gtatgattta aatggtcagt cgtctcggct attgaccttc ccaaccagac ataacaacag
      241 acaaaacctt tctaactctt ctccaaccag caacggttct tctttcacca acgtcaccct
      301 tccaaacaac cttgatttct ctaccgatca acaagtaaga agcacaagca gctctcatac
      361 cagcttgaac ttcggttggc aatctgtcga tacccttgta agagtgctta gccaagtctt
      421 cagcgtaagc aaccaatggc aaagagtagg tcttccattc gaatctgaaa gattcagaag
      481 cgttagaaga tggcaaggta gaagatggag acaaagacaa caacttgtcg aagtcttgtg
      541 gtcttggttc ggtccagtcg gttgggatag ccaacttaga ttcgtcgcgc aaaccgaaga
      601 aagacaatgg caagtagaat ctaccttcgg tagcgtcacc cttgatgtct ctagcgatgt
      661 taaccaattg caaagcggtt cccatttctc tactagcaac caaa
//
