LOCUS       sequence-137954-         806 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial pMCSG7 expression vector expressing Nme cas9 nickase with 
            a D16A mutation (RuvC domain) with T7 promoter, N-terminal His tag 
            and TEV site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 806)
  AUTHORS   Zhang Y, Rajan R, Seifert HS, Mondragon A, Sontheimer EJ
  TITLE     DNase H Activity of Neisseria meningitidis Cas9.
  JOURNAL   Mol Cell. 2015 Oct 15;60(2):242-55. doi: 
            10.1016/j.molcel.2015.09.020.
  PUBMED    26474066
REFERENCE   2  (bases 1 to 806)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2015.09"; journalName: "Mol Cell"; date: 
            "2015-10-15- 15"; volume: "60"; issue: "2"; pages: "242-55"
COMMENT     Sequence Label: pMCSG7-D16A-NmeCas9
FEATURES             Location/Qualifiers
     source          1..806
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(17..34)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tagcagccgg atctcagtgg tggtggtggt ggtgctcgag tgcggccgca agcttgtcga
       61 cggagctcga attcggatcc gttatccact tccaatgttt taacggacag gcgggcgttt
      121 tttcagacgg catggtctga tttctttgcc cagttcgtca atttggtatt tttggaatga
      181 aagggcggtt ttgacgccga taccttccag tattccattt ttgccaattt tatgatcaag
      241 atcatgaatg cgtatattga tattacctgt gcctcgatgg cagctggcaa agtaaccaaa
      301 cattctagct ttttttgtta taacctcgac taaatcatta gggtgtaatg agaatttaaa
      361 gttgaaacta tcatcaataa gttgccaatc ttcttcatct tttccttgta caacagccct
      421 atccggcaaa atccctttcg ctacctgcca actgtaaatc ggtaccagat aatacttgtc
      481 gcctttctca aacacatcta cgcgcaccat ggttgcgttg tcggcaatac cgttatggtt
      541 gcgcacccat acgccggttt tctgtacttg ctctacgcgt acggctttta cctgttgggt
      601 gcggttgcct gctttatcgt atttgtaaaa cggctcggca aaggctttgg caggatcgtc
      661 tttatgtgct tccagccgtg ctttcagtgc ttcgtatagc ttaggttcgc gctcccgatt
      721 gaccattttt tccaagtctt tcagttttaa ctgtgtcagc ggtacgcgca acacgctgac
      781 gccttcgtcc agtcgtttgg cggatt
//
