LOCUS       sequence-137965-        4755 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4755)
  AUTHORS   Johannessen CM, Boehm JS, Kim SY, Thomas SR, Wardwell L, Johnson LA,
            Emery CM, Stransky N, Cogdill AP, Barretina J, Caponigro G, 
            Hieronymus H, Murray RR, Salehi-Ashtiani K, Hill DE, Vidal M, Zhao 
            JJ, Yang X, Alkan O, Kim S, Harris JL, Wilson CJ, Myer VE, Finan PM,
            Root DE, Roberts TM, Golub T, Flaherty KT, Dummer R, Weber BL, 
            Sellers WR, Schlegel R, Wargo JA, Hahn WC, Garraway LA
  TITLE     COT drives resistance to RAF inhibition through MAP kinase pathway 
            reactivation.
  JOURNAL   Nature. 2010 Dec 16;468(7326):968-72. doi: 10.1038/nature09627
  PUBMED    21107320
REFERENCE   2  (bases 1 to 4755)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2010-12-16- 16"; volume: "468"; issue: "7326"; 
            pages: "968-72"
COMMENT     Sequence Label: pDONR223-SRPK1
FEATURES             Location/Qualifiers
     source          1..4755
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(149..176)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(268..354)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     403..425
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     417..434
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     418..434
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    450..549
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2515..2614)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2631..2650)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2632..2650)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2655..2671)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2655..2671)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3102..3893
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3986..4574
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4475..4494
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4728..4745
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat acgcgtaccg
      121 ctagccagga agagtttgta gaaacgcaaa aaggccatcc gtcaggatgg ccttctgctt
      181 agtttgatgc ctggcagttt atggcgggcg tcctgcccgc caccctccgg gccgttgctt
      241 cacaacgttc aaatccgctc ccggcggatt tgtcctactc aggagagcgt tcaccgacaa
      301 acaacagata aaacgaaagg cccagtcttc cgactgagcc tttcgtttta tttgatgcct
      361 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta
      421 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga
      481 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa
      541 aagttggcat ggagcggaaa gtgcttgcgc tccaggcccg aaagaaaagg accaaggcca
      601 agaaggacaa agcccaaagg aaatctgaaa ctcagcaccg aggctctgct ccccactctg
      661 agagtgatct accagagcag gaagaggaga ttctgggatc tgatgatgat gagcaagaag
      721 atcctaatga ttattgtaaa ggaggttatc atcttgtgaa aattggagat ctattcaatg
      781 ggagatacca tgtgatccga aagttaggct ggggacactt ttcaacagta tggttatcat
      841 gggatattca ggggaagaaa tttgtggcaa tgaaagtagt taaaagtgct gaacattaca
      901 ctgaaacagc actagatgaa atccggttgc tgaagtcagt tcgcaattca gaccctaatg
      961 atccaaatag agaaatggtt gttcaactac tagatgactt taaaatatca ggagttaatg
     1021 gaacacatat ctgcatggta tttgaagttt tggggcatca tctgctcaag tggatcatca
     1081 aatccaatta tcaggggctt ccactgcctt gtgtcaaaaa aattattcag caagtgttac
     1141 agggtcttga ttatttacat accaagtgcc gtatcaccca cactgacatt aaaccagaga
     1201 acatcttatt gtcagtgaat gagcagtaca ttcggaggct ggctgcagaa gcaacagaat
     1261 ggcagcgatc tggagctcct ccgccttccg gatctgcagt cagtactgct ccccagccta
     1321 aaccagctga caaaatgtca aagaataaga agaagaaatt gaagaagaag cagaagcgcc
     1381 aggcagaatt actagagaag cgaatgcagg aaattgagga aatggagaaa gagtcgggcc
     1441 ctgggcaaaa aagaccaaac aagcaagaag aatcagagag tcctgttgaa agacccttga
     1501 aagagaaccc acctaataaa atgacccaag aaaaacttga agagtcaagt accattggcc
     1561 aggatcaaac gcttatggaa cgtgatacag agggtggtgc agcagaaatt aattgcaatg
     1621 gagtgattga agtcattaat tatactcaga acagtaataa tgaaacattg agacataaag
     1681 aggatctaca taatgctaat gactgtgatg tccaaaattt gaatcaggaa tctagtttcc
     1741 taagctccca aaatggagac agcagcacat ctcaagaaac agactcttgt acacctataa
     1801 catctgaggt gtcagacacc atggtgtgcc agtcttcctc aactgtaggt cagtcattca
     1861 gtgaacaaca cattagccaa cttcaagaaa gcattcgggc agagataccc tgtgaagatg
     1921 aacaagagca agaacataac ggaccactgg acaacaaagg aaaatccacg gctggaaatt
     1981 ttcttgttaa tccccttgag ccaaaaaatg cagaaaagct caaggtgaag attgctgacc
     2041 ttggaaatgc ttgttgggtg cacaaacatt tcactgaaga tattcaaaca aggcaatatc
     2101 gttccttgga agttctaatc ggatctggct ataatacccc tgctgacatt tggagcacgg
     2161 catgcatggc ctttgaactg gccacaggtg actatttgtt tgaacctcat tcaggggaag
     2221 agtacactcg agatgaagat cacattgcat tgatcataga acttctgggg aaggtgcctc
     2281 gcaagctcat tgtggcagga aaatattcca aggaattttt caccaaaaaa ggtgacctga
     2341 aacatatcac gaagctgaaa ccttggggcc tttttgaggt tctagtggag aagtatgagt
     2401 ggtcgcagga agaggcagct ggcttcacag atttcttact gcccatgttg gagctgatcc
     2461 ctgagaagag agccactgcc gccgagtgtc tccggcaccc ttggcttaac tcctacccaa
     2521 ctttcttgta caaagttggc attataagaa agcattgctt atcaatttgt tgcaacgaac
     2581 aggtcactat cagtcaaaat aaaatcatta tttgccatcc agctgatatc ccctatagtg
     2641 agtcgtatta catggtcata gctgtttcct ggcagctctg gcccgtgtct caaaatctct
     2701 gatgttacat tgcacaagat aaaaatatat catcatgcct cctctagacc agccaggaca
     2761 gaaatgcctc gacttcgctg ctacccaagg ttgccgggtg acgcacaccg tggaaacgga
     2821 tgaaggcacg aacccagtgg acataagcct gttcggttcg taagctgtaa tgcaagtagc
     2881 gtatgcgctc acgcaactgg tccagaacct tgaccgaacg cagcggtggt aacggcgcag
     2941 tggcggtttt catggcttgt tatgactgtt tttttggggt acagtctatg cctcgggcat
     3001 ccaagcagca agcgcgttac gccgtgggtc gatgtttgat gttatggagc agcaacgatg
     3061 ttacgcagca gggcagtcgc cctaaaacaa agttaaacat tatgagggaa gcggtgatcg
     3121 ccgaagtatc gactcaacta tcagaggtag ttggcgtcat cgagcgccat ctcgaaccga
     3181 cgttgctggc cgtacatttg tacggctccg cagtggatgg cggcctgaag ccacacagtg
     3241 atattgattt gctggttacg gtgaccgtaa ggcttgatga aacaacgcgg cgagctttga
     3301 tcaacgacct tttggaaact tcggcttccc ctggagagag cgagattctc cgcgctgtag
     3361 aagtcaccat tgttgtgcac gacgacatca ttccgtggcg ttatccagct aagcgcgaac
     3421 tgcaatttgg agaatggcag cgcaatgaca ttcttgcagg tatcttcgag ccagccacga
     3481 tcgacattga tctggctatc ttgctgacaa aagcaagaga acatagcgtt gccttggtag
     3541 gtccagcggc ggaggaactc tttgatccgg ttcctgaaca ggatctattt gaggcgctaa
     3601 atgaaacctt aacgctatgg aactcgccgc ccgactgggc tggcgatgag cgaaatgtag
     3661 tgcttacgtt gtcccgcatt tggtacagcg cagtaaccgg caaaatcgcg ccgaaggatg
     3721 tcgctgccga ctgggcaatg gagcgcctgc cggcccagta tcagcccgtc atacttgaag
     3781 ctagacaggc ttatcttgga caagaagaag atcgcttggc ctcgcgcgca gatcagttgg
     3841 aagaatttgt ccactacgtg aaaggcgaga tcaccaaggt agtcggcaaa taaccctcga
     3901 gccacccatg accaaaatcc cttaacgtga gttacgcgtc gttccactga gcgtcagacc
     3961 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     4021 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     4081 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     4141 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     4201 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     4261 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     4321 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     4381 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     4441 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     4501 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     4561 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     4621 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     4681 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     4741 gcgaggaagc ggaag
//
