LOCUS       sequence-137972-       12687 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pZ8-1 plasmid carrying dcas9, driven by the IPTG-inducible Ptac 
            promoter, KanR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12687)
  AUTHORS   Cleto S, Jensen JV, Wendisch VF, Lu TK
  TITLE     Corynebacterium glutamicum Metabolic Engineering with CRISPR 
            Interference (CRISPRi).
  JOURNAL   ACS Synth Biol. 2016 Feb 16.
  PUBMED    26829286
REFERENCE   2  (bases 1 to 12687)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 Feb 16."
COMMENT     Sequence Label: pZ8-T_dCas9
FEATURES             Location/Qualifiers
     source          1..12687
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     268..290
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     promoter        368..445
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             446..1528
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPSTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(465..484)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        join(1645..1650,1651..1666,1667..1673)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    1681..1697
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1686..1708
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     CDS             1723..1788
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             1795..1815
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(1825..1851,1852..1854,1855..5928)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             5929..5949
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(6016..6033)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      6186..6272
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      6364..6391
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        6410..6501
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(6720..6739)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     CDS             complement(10446..11261)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIADALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     11169..11188
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     11253..11272
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(11722..11739)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11856..12401)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(11936..11955)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cggtacgcct gcggccaaat ttgattcctt tcagctttgc ttcctgtcgg ccctcattcg
       61 tgcgctctag gatcagtgat ccgggcttat cgactgcacg gtgcaccaat gcttctggcg
      121 tcaggcagcc atcggaagct gtggtatggc cgctatcgct acgtgactgg gtcatggctg
      181 cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat
      241 ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt
      301 catcaccgaa acgcgcgagg cagcagatca attcgcgcgc gaaggcgaag cggcatgcat
      361 ttacgttgac accatcgaat ggtgcaaaac ctttcgcggt atggcatgat agcgcccgga
      421 agagagtcaa ttcagggtgg tgaatgtgaa accagtaacg ttatacgatg tcgcagagta
      481 tgccggtgtc tcttatcaga ccgtttcccg cgtggtgaac caggccagcc acgtttctgc
      541 gaaaacgcgg gaaaaagtgg aagcggcgat ggcggagctg aattacattc ccaaccgcgt
      601 ggcacaacaa ctggcgggca aacagtcgtt gctgattggc gttgccacct ccagtctggc
      661 cctgcacgcg ccgtcgcaaa ttgtcgcggc gattaaatct cgcgccgatc aactgggtgc
      721 cagcgtggtg gtgtcgatgg tagaacgaag cggcgtcgaa gcctgtaaag cggcggtgca
      781 caatcttctc gcgcaacgcg tcagtgggct gatcattaac tatccgctgg atgaccagga
      841 tgccattgct gtggaagctg cctgcactaa tgttccggcg ttatttcttg atgtctctga
      901 ccagacaccc atcaacagta ttattttctc ccatgaagac ggtacgcgac tgggcgtgga
      961 gcatctggtc gcattgggtc accagcaaat cgcgctgtta gcgggcccat taagttctgt
     1021 ctcggcgcgt ctgcgtctgg ctggctggca taaatatctc actcgcaatc aaattcagcc
     1081 gatagcggaa cgggaaggcg actggagtgc catgtccggt tttcaacaaa ccatgcaaat
     1141 gctgaatgag ggcatcgttc ccactgcgat gctggttgcc aacgatcaga tggcgctggg
     1201 cgcaatgcgc gccattaccg agtccgggct gcgcgttggt gcggatatct cggtagtggg
     1261 atacgacgat accgaagaca gctcatgtta tatcccgccg tcaaccacca tcaaacagga
     1321 ttttcgcctg ctggggcaaa ccagcgtgga ccgcttgctg caactctctc agggccaggc
     1381 ggtgaagggc aatcagctgt tgcccgtctc actggtgaaa agaaaaacca ccctggcgcc
     1441 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
     1501 ggtttcccga ctggaaagcg ggcagtgact gtgcaggtcg taaatcactg cataattcgt
     1561 gtcgctcaag gcgcactccc gttctggata atgttttttg cgccgacatc ataacggttc
     1621 tggcaaatat tctgaaatga gctgttgaca attaatcatc ggctcgtata atgtgtggaa
     1681 ttgtgagcgg ataacaattt cacacaggaa acagaattca tggactacaa agaccatgac
     1741 ggtgattata aagatcatga catcgattac aaggatgacg atgacaagat ggcccccaag
     1801 aagaagagga aggtgggccg cggaatggat aagaaatact caataggctt agctatcggc
     1861 acaaatagcg tcggatgggc ggtgatcact gatgaatata aggttccgtc taaaaagttc
     1921 aaggttctgg gaaatacaga ccgccacagt atcaaaaaaa atcttatagg ggctctttta
     1981 tttgacagtg gagagacagc ggaagcgact cgtctcaaac ggacagctcg tagaaggtat
     2041 acacgtcgga agaatcgtat ttgttatcta caggagattt tttcaaatga gatggcgaaa
     2101 gtagatgata gtttctttca tcgacttgaa gagtcttttt tggtggaaga agacaagaag
     2161 catgaacgtc atcctatttt tggaaatata gtagatgaag ttgcttatca tgagaaatat
     2221 ccaactatct atcatctgcg aaaaaaattg gtagattcta ctgataaagc ggatttgcgc
     2281 ttaatctatt tggccttagc gcatatgatt aagtttcgtg gtcatttttt gattgaggga
     2341 gatttaaatc ctgataatag tgatgtggac aaactattta tccagttggt acaaacctac
     2401 aatcaattat ttgaagaaaa ccctattaac gcaagtggag tagatgctaa agcgattctt
     2461 tctgcacgat tgagtaaatc aagacgatta gaaaatctca ttgctcagct ccccggtgag
     2521 aagaaaaatg gcttatttgg gaatctcatt gctttgtcat tgggtttgac ccctaatttt
     2581 aaatcaaatt ttgatttggc agaagatgct aaattacagc tttcaaaaga tacttacgat
     2641 gatgatttag ataatttatt ggcgcaaatt ggagatcaat atgctgattt gtttttggca
     2701 gctaagaatt tatcagatgc tattttactt tcagatatcc taagagtaaa tactgaaata
     2761 actaaggctc ccctatcagc ttcaatgatt aaacgctacg atgaacatca tcaagacttg
     2821 actcttttaa aagctttagt tcgacaacaa cttccagaaa agtataaaga aatctttttt
     2881 gatcaatcaa aaaacggata tgcaggttat attgatgggg gagctagcca agaagaattt
     2941 tataaattta tcaaaccaat tttagaaaaa atggatggta ctgaggaatt attggtgaaa
     3001 ctaaatcgtg aagatttgct gcgcaagcaa cggacctttg acaacggctc tattccccat
     3061 caaattcact tgggtgagct gcatgctatt ttgagaagac aagaagactt ttatccattt
     3121 ttaaaagaca atcgtgagaa gattgaaaaa atcttgactt ttcgaattcc ttattatgtt
     3181 ggtccattgg cgcgtggcaa tagtcgtttt gcatggatga ctcggaagtc tgaagaaaca
     3241 attaccccat ggaattttga agaagttgtc gataaaggtg cttcagctca atcatttatt
     3301 gaacgcatga caaactttga taaaaatctt ccaaatgaaa aagtactacc aaaacatagt
     3361 ttgctttatg agtattttac ggtttataac gaattgacaa aggtcaaata tgttactgaa
     3421 ggaatgcgaa aaccagcatt tctttcaggt gaacagaaga aagccattgt tgatttactc
     3481 ttcaaaacaa atcgaaaagt aaccgttaag caattaaaag aagattattt caaaaaaata
     3541 gaatgttttg atagtgttga aatttcagga gttgaagata gatttaatgc ttcattaggt
     3601 acctaccatg atttgctaaa aattattaaa gataaagatt ttttggataa tgaagaaaat
     3661 gaagatatct tagaggatat tgttttaaca ttgaccttat ttgaagatag ggagatgatt
     3721 gaggaaagac ttaaaacata tgctcacctc tttgatgata aggtgatgaa acagcttaaa
     3781 cgtcgccgtt atactggttg gggacgtttg tctcgaaaat tgattaatgg tattagggat
     3841 aagcaatctg gcaaaacaat attagatttt ttgaaatcag atggttttgc caatcgcaat
     3901 tttatgcagc tgatccatga tgatagtttg acatttaaag aagacattca aaaagcacaa
     3961 gtgtctggac aaggcgatag tttacatgaa catattgcaa atttagctgg tagccctgct
     4021 attaaaaaag gtattttaca gactgtaaaa gttgttgatg aattggtcaa agtaatgggg
     4081 cggcataagc cagaaaatat cgttattgaa atggcacgtg aaaatcagac aactcaaaag
     4141 ggccagaaaa attcgcgaga gcgtatgaaa cgaatcgaag aaggtatcaa agaattagga
     4201 agtcagattc ttaaagagca tcctgttgaa aatactcaat tgcaaaatga aaagctctat
     4261 ctctattatc tccaaaatgg aagagacatg tatgtggacc aagaattaga tattaatcgt
     4321 ttaagtgatt atgatgtcga tcacattgtt ccacaaagtt tccttaaaga cgattcaata
     4381 gacaataagg tcttaacgcg ttctgataaa aatcgtggta aatcggataa cgttccaagt
     4441 gaagaagtag tcaaaaagat gaaaaactat tggagacaac ttctaaacgc caagttaatc
     4501 actcaacgta agtttgataa tttaacgaaa gctgaacgtg gaggtttgag tgaacttgat
     4561 aaagctggtt ttatcaaacg ccaattggtt gaaactcgcc aaatcactaa gcatgtggca
     4621 caaattttgg atagtcgcat gaatactaaa tacgatgaaa atgataaact tattcgagag
     4681 gttaaagtga ttaccttaaa atctaaatta gtttctgact tccgaaaaga tttccaattc
     4741 tataaagtac gtgagattaa caattaccat catgcccatg atgcgtatct aaatgccgtc
     4801 gttggaactg ctttgattaa gaaatatcca aaacttgaat cggagtttgt ctatggtgat
     4861 tataaagttt atgatgttcg taaaatgatt gctaagtctg agcaagaaat aggcaaagca
     4921 accgcaaaat atttctttta ctctaatatc atgaacttct tcaaaacaga aattacactt
     4981 gcaaatggag agattcgcaa acgccctcta atcgaaacta atggggaaac tggagaaatt
     5041 gtctgggata aagggcgaga ttttgccaca gtgcgcaaag tattgtccat gccccaagtc
     5101 aatattgtca agaaaacaga agtacagaca ggcggattct ccaaggagtc aattttacca
     5161 aaaagaaatt cggacaagct tattgctcgt aaaaaagact gggatccaaa aaaatatggt
     5221 ggttttgata gtccaacggt agcttattca gtcctagtgg ttgctaaggt ggaaaaaggg
     5281 aaatcgaaga agttaaaatc cgttaaagag ttactaggga tcacaattat ggaaagaagt
     5341 tcctttgaaa aaaatccgat tgacttttta gaagctaaag gatataagga agttaaaaaa
     5401 gacttaatca ttaaactacc taaatatagt ctttttgagt tagaaaacgg tcgtaaacgg
     5461 atgctggcta gtgccggaga attacaaaaa ggaaatgagc tggctctgcc aagcaaatat
     5521 gtgaattttt tatatttagc tagtcattat gaaaagttga agggtagtcc agaagataac
     5581 gaacaaaaac aattgtttgt ggagcagcat aagcattatt tagatgagat tattgagcaa
     5641 atcagtgaat tttctaagcg tgttatttta gcagatgcca atttagataa agttcttagt
     5701 gcatataaca aacatagaga caaaccaata cgtgaacaag cagaaaatat tattcattta
     5761 tttacgttga cgaatcttgg agctcccgct gcttttaaat attttgatac aacaattgat
     5821 cgtaaacgat atacgtctac aaaagaagtt ttagatgcca ctcttatcca tcaatccatc
     5881 actggtcttt atgaaacacg cattgatttg agtcagctag gaggtgaccc caagaagaag
     5941 aggaaggtgg gctgaccggg gatccgtcga cctgcagcca agcttctgtt ttggcggatg
     6001 agagaagatt ttcagcctga tacagattaa atcagaacgc agaagcggtc tgataaaaca
     6061 gaatttgcct ggcggcagta gcgcggtggt cccacctgac cccatgccga actcagaagt
     6121 gaaacgccgt agcgccgatg gtagtgtggg gtctccccat gcgagagtag ggaactgcca
     6181 ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt
     6241 tgtcggtgaa cgctctcctg agtaggacaa atccgccggg agcggatttg aacgttgcga
     6301 agcaacggcc cggagggtgg cgggcaggac gcccgccata aactgccagg catcaaatta
     6361 agcagaaggc catcctgacg gatggccttt ttgcgtttct acaaactctt ttgtttattt
     6421 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     6481 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     6541 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     6601 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     6661 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     6721 ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata
     6781 cactattctc agaatgactt ggttgagtac tgcggcgtcg ctgatcgccc tggcgacgtt
     6841 gtgcgggtgg cttgtccctg agggcgctgc gacagatagc taaaaatctg ggtcaggatc
     6901 gccgtagagc gcgcgtcgtc gattggaggc ttcccctttg gttgacggtc ttcaatcgct
     6961 ctacggcgat cctgacgctt ttttgttgcg taccgtcgat cgttttattt ctgtcgatcc
     7021 cgaaaaagtt tttgcctttt gtaaaaaact tctcggtcgc cccgcaaatt ttcgattcca
     7081 gattttttaa aaaccaagcc agaaatacga cacaccgttt gcagataatc tgtctttcgg
     7141 aaaaatcaag tgcgatacaa aatttttagc acccctgagc tgcgcaaagt cccgcttcgt
     7201 gaaaattttc gtgccgcgtg attttccgcc aaaaacttta acgaacgttc gttataatgg
     7261 tgtcatgacc ttcacgacga agtactaaaa ttggcccgaa tcatcagcta tggatctctc
     7321 tgatgtcgcg ctggagtccg acgcgctcga tgctgccgtc gatttaaaaa cggtgatcgg
     7381 atttttccga gctctcgata cgacggacgc gccagcatca cgagactggg ccagtgccgc
     7441 gagcgaccta gaaactctcg tggcggatct tgaggagctg gctgacgagc tgcgtgctcg
     7501 gccagcgcca ggaggacgca cagtagtgga ggatgcaatc agttgcgcct actgcggtgg
     7561 cctgattcct ccccggcctg acccgcgagg acggcgcgca aaatattgct cagatgcgtg
     7621 tcgtgccgca gccagccgcg agcgcgccaa caaacgccac gccgaggagc tggaggcggc
     7681 taggtcgcaa atggcgctgg aagtgcgtcc cccgagcgaa attttggcca tggtcgtcac
     7741 agagctggaa gcggcagcga gaattatccg cgatcgtggc gcggtgcccg caggcatgac
     7801 aaacatcgta aatgccgcgt ttcgtgtggc cgtggccgcc caggacgtgt cagcgccgcc
     7861 accacctgca ccgaatcggc agcagcgtcg cgcgtcgaaa aagcgcacag gcggcaagaa
     7921 gcgataagct gcacgaatac ctgaaaaatg ttgaacgccc cgtgagcggt aactcacagg
     7981 gcgtcggcta acccccagtc caaacctggg agaaagcgct caaaaatgac tctagcggat
     8041 tcacgagaca ttgacacacc ggcctggaaa ttttccgctg atctgttcga cacccatccc
     8101 gagctcgcgc tgcgatcacg tggctggacg agcgaagacc gccgcaaatt cctcgctcac
     8161 ctgggcagag aaaatttcca gggcagcaag acccgcgact tcgccagcgc ttggatcaaa
     8221 gacccggaca cgggagaaac acagccgaag ttataccgag ttggttcaaa atcgcttgcc
     8281 cggtgccagt atgttgctct gacgcacgcg cagcacgcag ccgtgcttgt cctggacatt
     8341 gatgtgccga gccaccaggc cggcgggaaa atcgagcacg taaaccccga ggtctacgcg
     8401 attttggagc gctgggcacg cctggaaaaa gcgccagctt ggatcggcgt gaatccactg
     8461 agcgggaaat gccagctcat ctggctcatt gatccggtgt atgccgcagc aggcatgagc
     8521 agcccgaata tgcgcctgct ggctgcaacg accgaggaaa tgacccgcgt tttcggcgct
     8581 gaccaggctt tttcacatag gctgagccgt ggccactgca ctctccgacg atcccagccg
     8641 taccgctggc atgcccagca caatcgcgtg gatcgcctag ctgatcttat ggaggttgct
     8701 cgcatgatct caggcacaga aaaacctaaa aaacgctatg agcaggagtt ttctagcgga
     8761 cgggcacgta tcgaagcggc aagaaaagcc actgcggaag caaaagcact tgccacgctt
     8821 gaagcaagcc tgccgagcgc cgctgaagcg tctggagagc tgatcgacgg cgtccgtgtc
     8881 ctctggactg ctccagggcg tgccgcccgt gatgagacgg cttttcgcca cgctttgact
     8941 gtgggatacc agttaaaagc ggctggtgag cgcctaaaag acaccaaggg tcatcgagcc
     9001 tacgagcgtg cctacaccgt cgctcaggcg gtcggaggag gccgtgagcc tgatctgccg
     9061 ccggactgtg accgccagac ggattggccg cgacgtgtgc gcggctacgt cgctaaaggc
     9121 cagccagtcg tccctgctcg tcagacagag acgcagagcc agccgaggcg aaaagctctg
     9181 gccactatgg gaagacgtgg cggtaaaaag gccgcagaac gctggaaaga cccaaacagt
     9241 gagtacgccc gagcacagcg agaaaaacta gctaagtcca gtcaacgaca agctaggaaa
     9301 gctaaaggaa atcgcttgac cattgcaggt tggtttatga ctgttgaggg agagactggc
     9361 tcgtggccga caatcaatga agctatgtct gaatttagcg tgtcacgtca gaccgtgaat
     9421 agagcactta aggtctgcgg gcattgaact tccacgagga cgccgaaagc ttcccagtaa
     9481 atgtgccatc tcgtaggcag aaaacggttc ccccgtaggg tctctctctt ggcctccttt
     9541 ctaggtcggg ctgattgctc ttgaagctct ctaggggggc tcacaccata ggcagataac
     9601 gttccccacc ggctcgcctc gtaagcgcac aaggactgct cccaaagatc ttcaaagcca
     9661 ctgccgcgac tgccttcgcg aagccttgcc ccgcggaaat ttcctccacc gagttcgtgc
     9721 acacccctat gccaagcttc tttcacccta aattcgagag attggattct taccgtggaa
     9781 attcttcgca aaaatcgtcc cctgatcgcc cttgcgacgt tggcgtcggt gccgctggtt
     9841 gcgcttggct tgaccgactt gatcctccgg cgttcagcct gtgccacagc cgacaggatg
     9901 gtgaccacca tttgccccat atcaccgtcg gtactgatcc cgtcgtcaat aaaccgaacc
     9961 gctacaccct gagcatcaaa ctcttttatc agttggatca tgtcggcgtg tcgcggccaa
    10021 gacggtcgag cttcttcacc agaatgacat caccttcctc caccttcatc ctcagcaaat
    10081 ccagcccttc ccgatctgtt gaactgccgg atgccttgtc ggtaaagatg cggttagctt
    10141 ttacccctgc atctttgagc gctgaggtct gcctcgtgaa gaaggtgttg ctgactcata
    10201 ccaggcctga atcgccccat catccagcca gaaagtgagg gagccacggt tgatgagagc
    10261 tttgttgtag gtggaccagt tggtgatttt gaacttttgc tttgccacgg aacggtctgc
    10321 gttgtcggga agatgcgtga tctgatcctt caactcagca aaagttcgat ttattcaaca
    10381 aagccgccgt cccgtcaagt cagcgtaatg ctctgccagt gttacaacca attaaccaat
    10441 tctgattaga aaaactcatc gagcatcaaa tgaaactgca atttattcat atcaggatta
    10501 tcaataccat atttttgaaa aagccgtttc tgtaatgaag gagaaaactc accgaggcag
    10561 ttccatagga tggcaagatc ctggtatcgg tctgcgattc cgactcgtcc aacatcaata
    10621 caacctatta atttcccctc gtcaaaaata aggttatcaa gtgagaaatc accatgagtg
    10681 acgactgaat ccggtgagaa tggcaaaagc ttatgcattt ctttccagac ttgttcaaca
    10741 ggccagccat tacgctcgtc atcaaaatca ctcgcatcaa ccaaaccgtt attcattcgt
    10801 gattgcgcct gagcgagacg aaatacgcga tcgctgttaa aaggacaatt acaaacagga
    10861 atcgaatgca accggcgcag gaacactgcc agcgcatcag caatattttc acctgaatca
    10921 ggatattctt ctaatacctg gaatgctgtt ttcccgggga tcgcagtggt gagtaaccat
    10981 gcatcatcag gagtacggat aaaatgcttg atggtcggaa gaggcataaa ttccgtcagc
    11041 cagtttagtc tgaccatctc atctgtaaca tcattggcaa cgctaccttt gccatgtttc
    11101 agaaacaact ctggcgcatc gggcttccca tacaatcgat agattgtcgc acctgattgc
    11161 ccgacattat cgcgagccca tttataccca tataaatcag catccatgtt ggaatttaat
    11221 cgcggcctcg agcaagacgt ttcccgttga atatggctca taacacccct tgtattactg
    11281 tttatgtaag cagacagttt tattgttcat gatgatatat ttttatcttg tgcaatgtaa
    11341 catcagagat tttgagacac aacgtggctt tgttgaataa atcgaacttt tgctgagttg
    11401 aaggatcaga tcacgcatct tcccgacaac gcagaccgtt ccgtggcaaa gcaaaagttc
    11461 aaaatcacca actggtccac ctacaacaaa gctctcatca accgtggctc cctcactttc
    11521 tggctggatg atggggcgat tcaggcctgg tatgagtcag caacaccttc ttcacgaggc
    11581 agacctcagc gctagcggag tgtatactgg cttactatgt tggcactgat gagggtgtca
    11641 gtgaagtgct tcatgtggca ggagaaaaaa ggctgcaccg gtgcgtcagc agaatatgtg
    11701 atacaggata tattccgctt cctcgctcac tgactcgcta cgctcggtcg ttcgactgcg
    11761 gcgagcggaa atggcttacg aacggggcgg agatttcctg gaagatgcca ggaagatact
    11821 taacagggaa gtgagagggc cgcggcaaag ccgtttttcc ataggctccg cccccctgac
    11881 aagcatcacg aaatctgacg ctcaaatcag tggtggcgaa acccgacagg actataaaga
    11941 taccaggcgt ttccccctgg cggctccctc gtgcgctctc ctgttcctgc ctttcggttt
    12001 accggtgtca ttccgctgtt atggccgcgt ttgtctcatt ccacgcctga cactcagttc
    12061 cgggtaggca gttcgctcca agctggactg tatgcacgaa ccccccgttc agtccgaccg
    12121 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gaaagacatg caaaagcacc
    12181 actggcagca gccactggta attgatttag aggagttagt cttgaagtca tgcgccggtt
    12241 aaggctaaac tgaaaggaca agttttggtg actgcgctcc tccaagccag ttacctcggt
    12301 tcaaagagtt ggtagctcag agaaccttcg aaaaaccgcc ctgcaaggcg gttttttcgt
    12361 tttcagagca agagattacg cgcagaccaa aacgatctca agaagatcat cttattaagg
    12421 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
    12481 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
    12541 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
    12601 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
    12661 tacgggaggg cttaccatct ggcccca
//
