LOCUS       sequence-137978-        2706 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expressing UAS-CD4-mIFP T2A HO1 in drosophila.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2706)
  AUTHORS   Yu D, Baird MA, Allen JR, Howe ES, Klassen MP, Reade A, Makhijani K,
            Song Y, Liu S, Murthy Z, Zhang SQ, Weiner OD, Kornberg TB, Jan YN, 
            Davidson MW, Shu X
  TITLE     A naturally monomeric infrared fluorescent protein for protein 
            labeling in vivo.
  JOURNAL   Nat Methods. 2015 Aug;12(8):763-765. doi: 10.1038/nmeth.3447. Epub 
            2015 Jun 22.
  PUBMED    26098020
REFERENCE   2  (bases 1 to 2706)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.3447"; journalName: "Nat Methods"; date: "2015-08"; 
            volume: "12"; issue: "8"; pages: "763-765"
COMMENT     Sequence Label: pACU2_CD4-mIFP T2A HO1
FEATURES             Location/Qualifiers
     source          1..2706
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             820..1779
                     /codon_start=1
                     /product="naturally monomeric infrared fluorescent protein 
                     derived from a truncated bacteriophytochrome (Yu et al., 
                     2015)"
                     /label=mIFP
                     /translation="MSVPLTTSAFGHAFLANCEREQIHLAGSIQPHGILLAVKEPDNVV
                     IQASINAAEFLNTNSVVGRPLRDLGGDLPLQILPHLNGPLHLAPMTLRCTVGSPPRRVD
                     CTIHRPSNGGLIVELEPATKTTNIAPALDGAFHRITSSSSLMGLCDETATIIREITGYD
                     RVMVVRFDEEGNGEILSERRRADLEAFLGNRYPASTIPQIARRLYEHNRVRLLVDVNYT
                     PVPLQPRISPLNGRDLDMSLSCLRSMSPIHQKYMQDMGVGATLVCSLMVSGRLWGLIAC
                     HHYEPRFVPFHIRAAGEALAETCAIRIATLESFAQSQSK"
     CDS             1783..1794
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             1786..1839
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 atgaatccca agagcgaagt cctcattgca gccgtgctgt tcatgctgct ggcctgcgtc
       61 cagtgtcaag ctagcggtgg cggcggaagt ggaggtggag gctcgttcca gaaggcctcc
      121 agcatagtct ataagaaaga gggggaacag gtggagttct ccttcccact cgcctttaca
      181 gttgaaaagc tgacgggcag tggcgagctg tggtggcagg cggagagggc ttcctcctcc
      241 aagtcttgga tcacctttga cctgaagaac aaggaagtgt ctgtaaaacg ggttacccag
      301 gaccctaagc tccagatggg caagaagctc ccgctccacc tcaccctgcc ccaggccttg
      361 cctcagtatg ctggctctgg aaacctcacc ctggcccttg aagcgaaaac aggaaagttg
      421 catcaggaag tgaacctggt ggtgatgaga gccactcagc tccagaaaaa tttgacctgt
      481 gaggtgtggg gacccacctc ccctaagctg atgctgagct tgaaactgga gaacaaggag
      541 gcaaaggtct cgaagcggga gaaggcggtg tgggtgctga accctgaggc ggggatgtgg
      601 cagtgtctgc tgagtgactc gggacaggtc ctgctggaat ccaacatcaa ggttctgccc
      661 acatggtcca ccccggtgca gccaatggcc ctgattgtgc tggggggcgt cgccggcctc
      721 ctgcttttca ttgggctagg catcttcttc tgtgtcaggt gccggcaccg aaggcgcggc
      781 ggaagcggag gcggatccat ggtgagcaag ggcgaggaga tgtcggtacc gctgactacc
      841 tcagcattcg gccacgcgtt tctggctaac tgtgaacgcg agcagatcca cctggcgggc
      901 tccattcagc cgcacggtat cctgctggct gtgaaagagc cggacaacgt ggtgatccag
      961 gcttctatta acgctgcgga gttcctgaac accaactctg ttgttggccg tccgctgcgt
     1021 gacctgggcg gcgatctgcc tttgcagatc ctgccgcacc tgaacggccc gctgcacctg
     1081 gctccgatga ccctgcgttg taccgtgggt tctccgccgc gtcgtgtgga ctgtaccatt
     1141 catcgtccgt ctaacggcgg cctgatcgta gaactggaac cagcaaccaa gaccactaac
     1201 attgcgccgg ctctggacgg tgcgtttcat cgtatcactt cttcatcctc cctgatgggc
     1261 ctgtgtgacg aaaccgcgac tattatccgt gagattactg gctacgaccg tgtgatggta
     1321 gtacgtttcg atgaagaggg taatggcgaa attctgtccg aacgtcgtcg tgcggacctg
     1381 gaagcgttcc tgggtaaccg ctacccggcg tctactattc cgcagatcgc tcgtcgcctg
     1441 tacgaacata accgtgttcg cctgctggta gatgtgaact atactccggt tccgctacag
     1501 ccgcgcatca gcccgctgaa cggtcgtgat ctggatatgt ccctgtcttg cctgcgctct
     1561 atgtccccga tccaccagaa atacatgcag gacatgggcg ttggcgcgac cctggtttgc
     1621 tctctgatgg tgtctggtcg tctgtggggt ctgatcgctt gccaccacta cgaaccgcgc
     1681 ttcgttccgt tccacattcg cgctgctggc gaagcgctgg cggaaacttg tgcgatccgc
     1741 atcgcgacgc tggagagctt tgcacagtct cagtccaaag atatcgaggg cagaggaagt
     1801 ctgctaacat gcggtgacgt cgaggagaat cctggcccaa tggaaagacc acagccagac
     1861 tctatgccac aggatctgag cgaagccctc aaggaggcca ccaaggaagt gcacacccag
     1921 gccgagaacg ctgagttcat gagaaacttc cagaaaggac aggttactcg cgacggattc
     1981 aagctggtga tggctagcct ctatcacatc tacgtggccc tggaggagga aattgagaga
     2041 aacaaggaat cccccgtgtt cgctccagtt tatttcccag aggagctgca tcggaaggcc
     2101 gccctggaac aggatctggc tttttggtat ggcccccgct ggcaggaggt cattccttat
     2161 acccccgcta tgcaacgcta cgttaagaga ctccacgagg tgggccgcac agaacctgag
     2221 ctgctcgtgg cccatgccta cacccgctac ctgggcgacc tgtcaggggg tcaagttctg
     2281 aaaaagatag cccaaaaggc cctggacctg ccttcctctg gcgaggggct ggccttcttt
     2341 actttcccta acattgcctc agccacaaag ttcaagcagc tctaccggtc caggatgaac
     2401 agcctggaaa tgaccccagc cgtgcggcag cgggtcatag aagaagccaa gactgccttt
     2461 ctgctgaata tccagctgtt tgaggaactc caggaactgc tgacccatga cactaaagac
     2521 cagtccccct ccagggcccc aggactcaga cagagggcct caaacaaggt tcaggactca
     2581 gcccccgtgg aaacacccag gggaaaaccc cctctcaata caagatctca agcacccctg
     2641 ctgagatggg tcctcaccct gagcttcctc gtcgctaccg ttgcagtggg gctctatgct
     2701 atgtga
//
