LOCUS       sequence-137979-        1017 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expressing iBlueberry and mCherry in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1017)
  AUTHORS   Yu D, Dong Z, Gustafson WC, Ruiz-Gonzalez R, Signor L, Marzocca F, 
            Borel F, Klassen MP, Makhijani K, Royant A, Jan YN, Weiss WA, Guo S,
            Shu X
  TITLE     Rational design of a monomeric and photostable far-red fluorescent 
            protein for fluorescence imaging in vivo.
  JOURNAL   Protein Sci. 2016 Feb;25(2):308-15. doi: 10.1002/pro.2843. Epub 2015
            Dec 31.
  PUBMED    26549191
REFERENCE   2  (bases 1 to 1017)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/pro.2843"; 
            journalName: "Protein Sci"; date: "2016-02"; volume: "25"; issue: 
            "2"; pages: "308-15"
COMMENT     Sequence Label: pcDNA3.1_LifeAct-iBlueberry
FEATURES             Location/Qualifiers
     source          1..1017
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..51
                     /codon_start=1
                     /product="peptide that binds filamentous actin (Riedl et 
                     al., 2008)"
                     /label=Lifeact
                     /note="first 17 amino acids of budding yeast Abp140"
                     /translation="MGVADLIKKFESISKEE"
     CDS             58..1017
                     /codon_start=1
                     /product="naturally monomeric infrared fluorescent protein 
                     derived from a truncated bacteriophytochrome (Yu et al., 
                     2015)"
                     /label=mIFP
                     /translation="MSVPLTTSAFGHAFLANCEREQIHLAGSIQPHGILLAVKEPDNVV
                     IQASINAAEFLNTNSVVGRPLRDLGGDLPLQILPHLNGPLHLAPMTLRCTVGSPPRRVD
                     CTIHRPSNGGLIVELEPATKTTNIAPALDGAFHRITSSSSLMGLCDETATIIREITGYD
                     RVMVVRFDEEGNGEILSERRRADLEAFLGNRYPASTIPQIARRLYEHNRVRLLVDVNYT
                     PVPLQPRISPLNGRDLDMSLSCLRSMSPCHQKYMQDMGVGATLVCSLMVSGRLWGLIAC
                     HHYEPRFVPFHIRAAGEALAETCAIRIATLESFAQSQSK"
ORIGIN
        1 atgggcgtgg ccgacttgat caagaagttc gagtccatct ccaaggagga gggcagcatg
       61 tcggtaccgc tgactacctc agcattcggc cacgcgtttc tggctaactg tgaacgcgag
      121 cagatccacc tggcgggctc cattcagccg cacggtatcc tgctggctgt gaaagagccg
      181 gacaacgtgg tgatccaggc ttctattaac gctgcggagt tcctgaacac caactctgtt
      241 gttggccgtc cgctgcgtga cctgggcggc gatctgcctt tgcagatcct gccgcacctg
      301 aacggcccgc tgcacctggc tccgatgacc ctgcgttgta ccgtgggttc tccgccgcgt
      361 cgtgtggact gtaccattca tcgtccgtct aacggcggcc tgatcgtaga actggaacca
      421 gcaaccaaga ccactaacat tgcgccggct ctggacggtg cgtttcatcg tatcacttct
      481 tcatcctccc tgatgggcct gtgtgacgaa accgcgacta ttatccgtga gattactggc
      541 tacgaccgtg tgatggtagt acgtttcgat gaagagggta atggcgaaat tctgtccgaa
      601 cgtcgtcgtg cggacctgga agcgttcctg ggtaaccgct acccggcgtc tactattccg
      661 cagatcgctc gtcgcctgta cgaacataac cgtgttcgcc tgctggtaga tgtgaactat
      721 actccggttc cgctacagcc gcgcatcagc ccgctgaacg gtcgtgatct ggatatgtcc
      781 ctgtcttgcc tgcgctctat gtccccgtgc caccagaaat acatgcagga catgggcgtt
      841 ggcgcgaccc tggtttgctc tctgatggtg tctggtcgtc tgtggggtct gatcgcttgc
      901 caccactacg aaccgcgctt cgttccgttc cacattcgcg ctgctggcga agcgctggcg
      961 gaaacttgtg cgatccgcat cgcgacgctg gagagctttg cacagtctca gtccaaa
//
