LOCUS       sequence-137991-         741 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Human expression vector containing MSCV LTR promoter, dCas9 that is 
            fused to 2x NLS, p65 activation domain and tagBFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 741)
  AUTHORS   Gilbert LA, Larson MH, Morsut L, Liu Z, Brar GA, Torres SE, 
            Stern-Ginossar N, Brandman O, Whitehead EH, Doudna JA, Lim WA, 
            Weissman JS, Qi LS
  TITLE     CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in 
            Eukaryotes.
  JOURNAL   Cell. 2013 Jul 9. pii: S0092-8674(13)00826-X. doi: 
            10.1016/j.cell.2013.06.044.
  PUBMED    23849981
REFERENCE   2  (bases 1 to 741)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2013 
            Jul 9. pii: S0092-8674(13)00826-X. doi: 10.1016/j.cell.2013.06.044."
COMMENT     Sequence Label: pMSCV-LTR-dCas9-p65AD-BFP
FEATURES             Location/Qualifiers
     source          1..741
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(10..366)
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALL"
ORIGIN
        1 aatctgtgac agcaaggctg agaaatccat atcggcgatg gaggagaaat cctcgtcgcc
       61 gctcaaaagt ccgttaggga ggccaggtgc ccccaaaggt gcgggtgcgg gatcgggagg
      121 ccgctgcgct cccgtcacaa gtctcgtgat tgcttcgggg tattccatca acatgggctc
      181 cgttgtatgt ggcgcgaccg ggataccctg gttcaaaagc tgctgaaatt cggagttatc
      241 tactgacgcg aggtcggtaa acaccgcagg gtctgtcgag tttccaagga gtgctccaag
      301 atcctcgtca tcaaactgga gttgaagaag tgcttcggag agtgttccct ctccggcctg
      361 cgtcggcttc ggagccggtg gagcgaccgc ctggggcggt ccaggcgcga gcacggggac
      421 gggcgcaggg gcctgtgcga gggcggacac cattgccggt gcaggcgctg gggcaggcgc
      481 ttgcggaagg acttggggag gcgctggtgc gagcgccgag gcttgtgaga tttgtcccga
      541 ggggaacacc atcgtcggga actcgtcata attgattgtg ctcaggctcg acgtgaaggg
      601 gtagggctgc ggcgcaggct tcggcacgct ggccgacgag cgggacggga ccgcaatcct
      661 tctcggtggg ggcctgggat cggtcggacc cgagaagggc gatttcttca tgatcgattt
      721 gaaggtttca tacgttcgct t
//
