LOCUS       sequence-137992-         794 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Human expression vector containing MSCV LTR promoter, dCas9 that is 
            fused to 2x NLS, p65 activation domain and tagBFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 794)
  AUTHORS   Gilbert LA, Larson MH, Morsut L, Liu Z, Brar GA, Torres SE, 
            Stern-Ginossar N, Brandman O, Whitehead EH, Doudna JA, Lim WA, 
            Weissman JS, Qi LS
  TITLE     CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in 
            Eukaryotes.
  JOURNAL   Cell. 2013 Jul 9. pii: S0092-8674(13)00826-X. doi: 
            10.1016/j.cell.2013.06.044.
  PUBMED    23849981
REFERENCE   2  (bases 1 to 794)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2013 
            Jul 9. pii: S0092-8674(13)00826-X. doi: 10.1016/j.cell.2013.06.044."
COMMENT     Sequence Label: pMSCV-LTR-dCas9-p65AD-BFP
FEATURES             Location/Qualifiers
     source          1..794
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      37..46
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ccctcactcc ttctctaggc gccggaatta gatctcgcca ccatggacaa gaagtattct
       61 atcggactgg ccatcgggac taatagcgtc gggtgggccg tgatcactga cgagtacaag
      121 gtgccctcta agaagttcaa ggtgctcggg aacaccgacc ggcattccat caagaaaaat
      181 ctgatcggag ctctcctctt tgattcaggg gagaccgctg aagcaacccg cctcaagcgg
      241 actgctagac ggcggtacac caggaggaag aaccggattt gttaccttca agagatattc
      301 tccaacgaaa tggcaaaggt cgacgacagc ttcttccata ggctggaaga atcattcctc
      361 gtggaagagg ataagaagca tgaacggcat cccatcttcg gtaatatcgt cgacgaggtg
      421 gcctatcacg agaaataccc aaccatctac catcttcgca aaaagctggt ggactcaacc
      481 gacaaggcag acctccggct tatctacctg gccctggccc acatgatcaa gttcagaggc
      541 cacttcctga tcgagggcga cctcaatcct gacaatagcg atgtggataa actgttcatc
      601 cagctggtgc agacttacaa ccagctcttt gaagagaacc ccatcaatgc aagcggagtc
      661 gatgccaagg ccattctgtc agcccggctg tcaaagagcc gcagacttga gaatcttatc
      721 gctcagctgc cgggtgaaaa gaaaaatgga ctgttcggga acctgattgc tctttcactt
      781 gggctgactc ccaa
//
