LOCUS       sequence-137993-         635 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd gen lentiviral backbone for cloning and expression of new shRNA 
            sequences. Uses blasticidin for selection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 635)
  AUTHORS   Bryant DM, Datta A, Rodriguez-Fraticelli AE, Peranen J, 
            Martin-Belmonte F, Mostov KE
  TITLE     A molecular network for de novo generation of the apical surface and
            lumen.
  JOURNAL   Nat Cell Biol. 2010 Nov;12(11):1035-45. doi: 10.1038/ncb2106. Epub 
            2010 Oct 3.
  PUBMED    20890297
REFERENCE   2  (bases 1 to 635)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ncb2106"; 
            journalName: "Nat Cell Biol"; date: "2010-11"; volume: "12"; issue: 
            "11"; pages: "1035-45"
COMMENT     Sequence Label: pLKO.1-blast
FEATURES             Location/Qualifiers
     source          1..635
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        114..362
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     114..134
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     285..304
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_feature    425..542
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 aaggaataga agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg
       61 gatctcgacg gtatcgatca cgagactagc ctcgagcggc cgcccccttc accgagggcc
      121 tatttcccat gattccttca tatttgcata tacgatacaa ggctgttaga gagataattg
      181 gaattaattt gactgtaaac acaaagatat tagtacaaaa tacgtgacgt agaaagtaat
      241 aatttcttgg gtagtttgca gttttaaaat tatgttttaa aatggactat catatgctta
      301 ccgtaacttg aaagtatttc gatttcttgg ctttatatat cttgtggaaa ggacgaaaca
      361 ccggtccgca ggtatgcacg cgtgaattct cgacctcgag acaaatggca gtattcatcc
      421 acaattttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca
      481 taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt
      541 ttcgggttta ttacagggac agcagagatc cactttggcc gcggctcgag ggggttgggg
      601 ttgcgccttt tccaaggcag ccctgggttt gcgca
//
