LOCUS       sequence-137996-       10593 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10593)
  AUTHORS   Egan DF, Shackelford DB, Mihaylova MM, Gelino S, Kohnz RA, Mair W, 
            Vasquez DS, Joshi A, Gwinn DM, Taylor R, Asara JM, Fitzpatrick J, 
            Dillin A, Viollet B, Kundu M, Hansen M, Shaw RJ
  TITLE     Phosphorylation of ULK1 (hATG1) by AMP-activated protein kinase 
            connects energy sensing to mitophagy.
  JOURNAL   Science. 2011 Jan 28;331(6016):456-61. doi: 10.1126/science.1196371.
            Epub 2010 Dec 23.
  PUBMED    21205641
REFERENCE   2  (bases 1 to 10593)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.1196371"; journalName: "Science"; date: 
            "2011-01-28- 28"; volume: "331"; issue: "6016"; pages: "456-61"
COMMENT     Sequence Label: pQCXIN- myc ULK1 K46I
FEATURES             Location/Qualifiers
     source          1..10593
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(11..28)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(182..770)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(262..281)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(941..1732,1733..1801))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1564..1583
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1802..1906)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1974..1992
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     enhancer        2092..2471
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2472..2675
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             2676..2851
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     misc_feature    2914..3113
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             3314..3730
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     3660..3682
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     3698..3714
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     promoter        4075..4278
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     protein_bind    4280..4298
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4301..4319
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4360..4384
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4393..4422
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    complement(7587..7611)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    7671..8237
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7831..7848)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     8058..8077
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             8251..9045
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MAEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8305..8324)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8915..8934
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     LTR             9462..9887
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     Moloney murine leukemia virus"
     primer_bind     complement(9990..10012)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(10148..10168)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10153..10482
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10333..10468
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     10504..10523
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
       61 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
      121 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
      181 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
      241 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
      301 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
      361 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
      421 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
      481 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
      541 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
      601 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta
      661 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
      721 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
      781 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
      841 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
      901 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
      961 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     1021 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     1081 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     1141 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     1201 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctgcag
     1261 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     1321 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     1381 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     1441 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     1501 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaacac
     1561 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     1621 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     1681 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     1741 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     1801 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     1861 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     1921 aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc
     1981 gtatcacgag gccctttcgt cttcaagaat tagcttggcc attgcatacg ttgtatccat
     2041 atcataatat gtacatttat attggctcat gtccaacatt accgccatgt tgacattgat
     2101 tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg
     2161 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
     2221 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt
     2281 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
     2341 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
     2401 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
     2461 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
     2521 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
     2581 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
     2641 ggcgtgtacg gtgggaggtc tatataagca gagctcaata aaagagccca caacccctca
     2701 ctcggcgcgc cagtcttccg atagactgcg tcgcccgggt acccgtattc ccaataaagc
     2761 ctcttgctgt ttgcatccga atcgtggtct cgctgttcct tgggagggtc tcctctgagt
     2821 gattgactac ccacgacggg ggtctttcat ttgggggctc gtccgggatt tggagacccc
     2881 tgcccaggga ccaccgaccc accaccggga ggtaagctgg ccagcaactt atctgtgtct
     2941 gtccgattgt ctagtgtcta tgtttgatgt tatgcgcctg cgtctgtact agttagctaa
     3001 ctagctctgt atctggcgga cccgtggtgg aactgacgag ttctgaacac ccggccgcaa
     3061 ccctgggaga cgtcccaggg actttggggg ccgtttttgt ggcccgacct gaggaaggga
     3121 gtcgatgtgg aatccgaccc cgtcaggata tgtggttctg gtaggagacg agaacctaaa
     3181 acagttcccg cctccgtctg aatttttgct ttcggtttgg aaccgaagcc gcgcgtcttg
     3241 tctgctgcag cgctgcagca tcgttctgtg ttgtctctgt ctgactgtgt ttctgtattt
     3301 gtctgaaaat tagggccaga ctgttaccac tcccttaagt ttgaccttag gtcactggaa
     3361 agatgtcgag cggatcgctc acaaccagtc ggtagatgtc aagaagagac gttgggttac
     3421 cttctgctct gcagaatggc caacctttaa cgtcggatgg ccgcgagacg gcacctttaa
     3481 ccgagacctc atcacccagg ttaagatcaa ggtcttttca cctggcccgc atggacaccc
     3541 agaccaggtc ccctacatcg tgacctggga agccttggct tttgaccccc ctccctgggt
     3601 caagcccttt gtacacccta agcctccgcc tcctcttcct ccatccgccc cgtctctccc
     3661 ccttgaacct cctcgttcga ccccgcctcg atcctccctt tatccagccc tcactccttc
     3721 tctaggcgcc ggaattgaag atctggggga tcgataagct tgggagttcc gcttacataa
     3781 cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata
     3841 atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag
     3901 tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc
     3961 cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta
     4021 tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg
     4081 cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt
     4141 ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca
     4201 aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag
     4261 gtctatataa gcagagctct ccctatcagt gatagagatc tccctatcag tgatagagat
     4321 cgtcgactag tccagtgtgg tggaattctg cagatatcaa caagtttgta caaaaaagca
     4381 ggcttcacca tggaacaaaa gctaatctcc gaggaagact tgggaggaat agagccgggc
     4441 cgaggcggcg tcgagaccgt gggcaagttc gagttctctc gcaaggacct gattggacac
     4501 ggcgccttcg cggtggtctt caagggtcga caccgcgaga agcacgacct ggaggtggcc
     4561 gtcatatgca ttaacaagaa gaaccttgcc aagtcccaaa cactgctggg aaaggaaatc
     4621 aaaatcctga aggaactaaa gcacgaaaac atcgtggcgc tgtatgactt ccaggaaatg
     4681 gctaattctg tctacctggt catggagtat tgtaatggtg gagacctggc tgactacctg
     4741 cacactatgc gcacactgag tgaagacact gtcaggcttt tcctacagca gatcgctggc
     4801 gccatgcggc tgctgcacag caagggcatc atccaccggg acctgaagcc ccaaaacatc
     4861 ctgctgtcca accctggggg ccgccgggcc aaccccagca acatccgagt caagattgct
     4921 gactttggat tcgctcggta cctccagagc aacatgatgg cggccacact ctgtggttct
     4981 cctatgtaca tggctcctga ggtcattatg tcccagcact acgatggaaa ggctgacctg
     5041 tggagcattg gcaccattgt ctaccagtgt ctgacaggga aggccccttt tcaggccagc
     5101 agccctcagg atttgcgcct gttttatgag aagaacaaga cactagttcc tgccatcccc
     5161 cgggagacat cagctcccct gcggcagctg ctcctggctc tgttgcagcg gaaccacaag
     5221 gaccgcatgg actttgatga atttttccac caccctttct tggatgccag cacccccatc
     5281 aagaaatccc cacctgtgcc tgtgccctca tatccaagct cagggtctgg cagcagctcc
     5341 agcagcagct ctgcctccca cctggcctct ccaccgtccc tgggggagat gccacagcta
     5401 cagaagaccc ttacctcccc agccgatgct gctggctttc ttcagggctc ccgggactct
     5461 ggtggcagca gcaaagactc ctgtgacaca gatgactttg tcatggtccc agcccagttt
     5521 ccaggtgatc tagttgctga ggcagccagt gccaagcccc cacctgatag cctgctgtgt
     5581 agtgggagct cattggtggc ctctgctggc ctagagagcc acggccgtac cccctctccc
     5641 tctccgacct gcagcagctc tcccagcccc tctggccggc ctggcccctt ctccagcaac
     5701 aggtacggtg cctcggtccc cattcctgtc cccactcagg tgcacaatta ccagcgcatc
     5761 gagcaaaacc tgcaatcgcc cactcaacag cagacagcca ggtcctctgc catccgaagg
     5821 tcagggagca ccagccccct gggctttggc cgggccagcc catcaccccc ctcccacacc
     5881 gatggagcca tgctggccag gaagctgtca cttggaggtg gccgtcccta cacaccttct
     5941 ccccaagtgg gaaccatccc agagcgaccc agctggagca gagtgccctc cccacaagga
     6001 gctgatgtgc gggttggcag gtcaccacga cccggttcct ctgtgcctga gcactctcca
     6061 agaaccactg ggctgggctg ccgcctgcac agtgccccta acctgtccga cttccatgtt
     6121 gtgcgtccca agctgcctaa gcccccaaca gacccactgg gagccacctt tagcccaccc
     6181 cagaccagcg caccccagcc atgcccaggg ctacagtctt gccggccact gcgtggctca
     6241 cctaagctgc ctgacttcct acagcggagt cccctacccc ccatcctagg ctctcctacc
     6301 aaggccgggc cctcctttga cttccccaaa acccccagct ctcagaattt gctgaccctg
     6361 ttggctaggc agggggtagt aatgacacca cctcggaacc gtacactgcc tgacctctcc
     6421 gaggccagtc ctttccatgg ccagcagctg ggctctggcc ttcggcccgc tgaagacacc
     6481 cggggtccct ttggacggtc cttcagcacc agccgcatta cggacctgct gcttaaggct
     6541 gcatttggga ctcaggcctc tgactcaggc agcacagaca gcctacagga gaaacctatg
     6601 gagattgctc cctctgctgg ctttggaggg actctgcatc caggagctcg tggtggaggg
     6661 gccagcagcc cagcacctgt ggtatttact gtaggctccc cacccagtgg tgccacccca
     6721 ccccagagta cccgtaccag aatgttctca gtgggctctt ccagctccct gggctctact
     6781 ggctcctcct ctgcccgcca cttagtgcct ggggcctgtg gagaggcccc ggagctttct
     6841 gccccaggcc actgctgtag ccttgctgac ccccttgctg ccaacttgga gggggctgtg
     6901 accttcgagg ctcctgacct cccagaggag accctcatgg agcaagagca cacggaaacc
     6961 ctacacagtc tgcgcttcac actagcgttt gcacagcaag ttctggagat tgcagccctg
     7021 aagggaagtg ccagtgaggc cgccggtggc cctgagtacc agctccagga aagtgtggtg
     7081 gctgaccaga tcagtcagtt gagccgagag tggggctttg cagagcaact ggttctgtac
     7141 ttgaaggtgg ctgagctgct gtcctcaggc ctacagactg ccattgacca gattcgagct
     7201 ggcaaactct gcctttcatc tactgtgaag caggtggtac gcagactaaa tgagctgtac
     7261 aaggccagcg tggtatcctg ccagggcctc agcttgcgac ttcagcgctt ctttctggac
     7321 aaacaacggc tgctggacgg gatccatggt gtcactgcag agcggctcat cctcagccat
     7381 gctgtgcaaa tggtacaatc agctgccctt gatgagatgt tccagcaccg agagggctgt
     7441 gtaccgagat atcacaaagc cctgctattg ctggaggggt tgcagcacac tctcacggac
     7501 caggcagaca ttgagaacat tgccaaatgc aagctgtgca ttgagaggag actctcggcc
     7561 ctgctgagtg gtgtctatgc ctgaataccc agctttcttg tacaaagtgg ttgatatcca
     7621 gcacagtggc gaccggtagg cctcgtacgc ttaattaacg gatccggaat tccgcccccc
     7681 cccctaacgt tactggccga agccgcttgg aataaggccg gtgtgcgttt gtctatatgt
     7741 tattttccac catattgccg tcttttggca atgtgagggc ccggaaacct ggccctgtct
     7801 tcttgacgag cattcctagg ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga
     7861 atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag acaaacaacg tctgtagcga
     7921 ccctttgcag gcagcggaac cccccacctg gcgacaggtg cctctgcggc caaaagccac
     7981 gtgtataaga tacacctgca aaggcggcac aaccccagtg ccacgttgtg agttggatag
     8041 ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg aaggatgccc
     8101 agaaggtacc ccattgtatg ggatctgatc tggggcctcg gtgcacatgc tttacatgtg
     8161 tttagtcgag gttaaaaaaa cgtctaggcc ccccgaacca cggggacgtg gttttccttt
     8221 gaaaaacacg atgataagct tgccacaacc atggctgaac aagatggatt gcacgcaggt
     8281 tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc
     8341 tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag
     8401 accgacctgt ccggtgccct gaatgaactg caggacgagg cagcgcggct atcgtggctg
     8461 gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac
     8521 tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc
     8581 gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc
     8641 tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc
     8701 ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg
     8761 ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat
     8821 gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc
     8881 cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa
     8941 gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat
     9001 tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg atcggctaga
     9061 gtcgacccgg acgggacgct cctgcgcctg atacagaacg aattgcttgc aggcatctca
     9121 tgagtgtgtc ttcccgtttt ccgcctgagg tcactgcgtg gatggagcgc tggcgcctgc
     9181 tgcgcgacgg cgagctgctc accacccact cgagggctgc agcgctgcag aggccgagtg
     9241 cagaactgct ccaaagggac ctcaaggctt tccgagggac actaggctga ctccatcgag
     9301 ccagtgtaga gataagctta tcgattagtc caatttgtta aagacaggat atcagtggtc
     9361 caggctctag ttttgactca acaatatcac cagctgaagc ctatagagta cgagccatag
     9421 ataaaataaa agattttatt tagtctccag aaaaaggggg gaatgaaaga ccccacctgt
     9481 aggtttggca agctagctta agtaacgcca ttttgcaagg catggaaaaa tacataactg
     9541 agaatagaga agttcagatc aaggtcagga acagatggaa cagggtcgac cctagagaac
     9601 catcagatgt ttccagggtg ccccaaggac ctgaaatgac cctgtgcctt atttgaacta
     9661 accaatcagt tcgcttctcg cttctgttcg cgcgcttctg ctccccgagc tcaataaaag
     9721 agcccacaac ccctcactcg gggcgccagt cctccgattg actgagtcgc ccgggtaccc
     9781 gtgtatccaa taaaccctct tgcagttgca tccgacttgt ggtctcgctg ttccttggga
     9841 gggtctcctc tgagtgattg actacccgtc agcgggggtc tttcatttgg gggctcgtcc
     9901 gggatcggga gacccctgcc cagggaccac cgacccacca ccgggaggta agctggctgc
     9961 ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc
    10021 acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt
    10081 gttggcgggt gtcggggcgc agccatgacc cagtcacgta gcgatagcgg agtgtagatc
    10141 cggctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga
    10201 agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc
    10261 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc
    10321 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
    10381 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
    10441 aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagcttac tggcttaact
    10501 atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca
    10561 gatgcgtaag gagaaaatac cgcatcaggc gct
//
