LOCUS       sequence-137997-         644 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  aTc-inducible expression of wild-type Cas9 (S .pyogenes) for 
            bacterial gene knockdown.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 644)
  AUTHORS   Qi LS, Larson MH, Gilbert LA, Doudna JA, Weissman JS, Arkin AP, Lim 
            WA
  TITLE     Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific 
            Control of Gene Expression.
  JOURNAL   Cell. 2013 Feb 28;152(5):1173-83. doi: 10.1016/j.cell.2013.02.022.
  PUBMED    23452860
REFERENCE   2  (bases 1 to 644)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.02"; journalName: "Cell"; date: "2013-02-28- 
            28"; volume: "152"; issue: "5"; pages: "1173-83"
COMMENT     Sequence Label: pwtCas9-bacteria
FEATURES             Location/Qualifiers
     source          1..644
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        48..103
                     /gene="tetR"
                     /label=tetR/tetA promoters
                     /note="overlapping promoters for bacterial tetR and tetA"
     protein_bind    84..102
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     RBS             121..132
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 atcactttac ttttatctaa tctagacatc attaattcct aatttttgtt gacactctat
       61 cgttgataga gttattttac cactccctat cagtgataga gaaaagaatt caaaagatct
      121 aaagaggaga aaggatctat ggataagaaa tactcaatag gcttagatat cggcacaaat
      181 agcgtcggat gggcggtgat cactgatgaa tataaggttc cgtctaaaaa gttcaaggtt
      241 ctgggaaata cagaccgcca cagtatcaaa aaaaatctta taggggctct tttatttgac
      301 agtggagaga cagcggaagc gactcgtctc aaacggacag ctcgtagaag gtatacacgt
      361 cggaagaatc gtatttgtta tctacaggag attttttcaa atgagatggc gaaagtagat
      421 gatagtttct ttcatcgact tgaagagtct tttttggtgg aagaagacaa gaagcatgaa
      481 cgtcatccta tttttggaaa tatagtagat gaagttgctt atcatgagaa atatccaact
      541 atctatcatc tgcgaaaaaa attggtagat tctactgata aagcggattt gcgcttaatc
      601 tatttggcct tagcgcatat gattaagttt cgtggtcatt tttt
//
