LOCUS       sequence-137998-         801 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  aTc-inducible expression of wild-type Cas9 (S .pyogenes) for 
            bacterial gene knockdown.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 801)
  AUTHORS   Qi LS, Larson MH, Gilbert LA, Doudna JA, Weissman JS, Arkin AP, Lim 
            WA
  TITLE     Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific 
            Control of Gene Expression.
  JOURNAL   Cell. 2013 Feb 28;152(5):1173-83. doi: 10.1016/j.cell.2013.02.022.
  PUBMED    23452860
REFERENCE   2  (bases 1 to 801)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.02"; journalName: "Cell"; date: "2013-02-28- 
            28"; volume: "152"; issue: "5"; pages: "1173-83"
COMMENT     Sequence Label: pwtCas9-bacteria
FEATURES             Location/Qualifiers
     source          1..801
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(5..51)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 acagataaaa cgaaaggccc agtctttcga ctgagccttt cgttttattt gatgcctgga
       61 gatccttact cgagttagtc acctcctagc tgactcaaat caatgcgtgt ttcataaaga
      121 ccagtgatgg attgatggat aagagtggca tctaaaactt cttttgtaga cgtatatcgt
      181 ttacgatcaa ttgttgtatc aaaatattta aaagcagcgg gagctccaag attcgtcaac
      241 gtaaataaat gaataatatt ttctgcttgt tcacgtattg gtttgtctct atgtttgtta
      301 tatgcactaa gaactttatc taaattggca tctgctaaaa taacacgctt agaaaattca
      361 ctgatttgct caataatctc atctaaataa tgcttatgct gctccacaaa caattgtttt
      421 tgttcgttat cttctggact acccttcaac ttttcataat gactagctaa atataaaaaa
      481 ttcacatatt tgcttggcag agccagctca tttccttttt gtaattctcc ggcactagcc
      541 agcatccgtt tacgaccgtt ttctaactca aaaagactat atttaggtag tttaatgatt
      601 aagtcttttt taacttcctt atatccttta gcttctaaaa agtcaatcgg atttttttca
      661 aaggaacttc tttccataat tgtgatccct agtaactctt taacggattt taacttcttc
      721 gatttccctt tttccacctt agcaaccact aggactgaat aagctaccgt tggactatca
      781 aaaccaccat attttttttg g
//
