LOCUS       sequence-138014-        1009 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Provides the Arabidopsis thaliana Actin2 gene terminator as a level 
            0 GoldenBraid part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1009)
  AUTHORS   Sarrion-Perdigones A, Vazquez-Vilar M, Palaci J, Castelijns B, 
            Forment J, Ziarsolo P, Blanca J, Granell A, Orzaez D
  TITLE     GoldenBraid 2.0: a comprehensive DNA assembly framework for plant 
            synthetic biology.
  JOURNAL   Plant Physiol. 2013 Jul;162(3):1618-31.
  PUBMED    23669743
REFERENCE   2  (bases 1 to 1009)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2013-07"; volume: "162(3)"; pages: "1618-31"
COMMENT     Sequence Label: pTAct2 (GB0210)
FEATURES             Location/Qualifiers
     source          1..1009
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      6..15
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      10..19
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(817..835)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(818..835)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(853..869)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(853..869)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(866..888)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    877..893
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(901..907,908..925,926..931))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    946..967
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 tcccggccgc catggcggcc gcgggaattc gattgggcga tgagtggtct cggcttgctc
       61 tcaagatcaa aggcttaaaa agctggggtt ttatgaatgg gatcaaagtt tctttttttc
      121 ttttatattt gcttctccat ttgtttgttt catttccctt tttgttttcg tttctatgat
      181 gcacttgtgt gtgacaaact ctctgggttt ttacttacgt ctgcgtttca aaaaaaaaaa
      241 ccgctttcgt tttgcgtttt agtcccattg ttttgtagct ctgagtgatc gaattgatgc
      301 ctctttattc cttttgttcc ctataatttc tttcaaaact cagaagaaaa accttgaaac
      361 tctttgcaat gttaatataa gtattgtata agatttttat tgatttggtt attagtctta
      421 cttttgctac ctccatcttc acttggaact gatattctga atagttaaag cgttacatgt
      481 cttccattca caaatgaact taaactagca caaagtcaga tattttaaga tcgcaccatt
      541 ttatataacc ccaatcgtca attctactgt ttcaagtttt acaccaaaac aattacgagg
      601 tgtatctatt cgttactctt ttcgcttatt aatcctttta catgatgcaa aatataacaa
      661 attaatttca aattgtcctg cacatttggt ttatacatct tgattccaaa aagtcgctcg
      721 agaccactca tcgcccaatc actagtgaat tcgcggccgc ctgcaggtcg accatatggg
      781 agagctccca acgcgttgga tgcatagctt gagtattcta tagtgtcacc taaatagctt
      841 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
      901 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
      961 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctg
//
