LOCUS       sequence-138015-        1023 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Provides the Arabidopsis thaliana Actin2 gene terminator as a level 
            0 GoldenBraid part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Sarrion-Perdigones A, Vazquez-Vilar M, Palaci J, Castelijns B, 
            Forment J, Ziarsolo P, Blanca J, Granell A, Orzaez D
  TITLE     GoldenBraid 2.0: a comprehensive DNA assembly framework for plant 
            synthetic biology.
  JOURNAL   Plant Physiol. 2013 Jul;162(3):1618-31.
  PUBMED    23669743
REFERENCE   2  (bases 1 to 1023)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2013-07"; volume: "162(3)"; pages: "1618-31"
COMMENT     Sequence Label: pTAct2 (GB0210)
FEATURES             Location/Qualifiers
     source          1..1023
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      773..782
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      777..786
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(816..835)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(817..835)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(842..859)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(842..858)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(851..873)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 ttgggagctc tcccatatgg tcgacctgca ggcggccgcg aattcactag tgattgggcg
       61 atgagtggtc tcgagcgact ttttggaatc aagatgtata aaccaaatgt gcaggacaat
      121 ttgaaattaa tttgttatat tttgcatcat gtaaaaggat taataagcga aaagagtaac
      181 gaatagatac acctcgtaat tgttttggtg taaaacttga aacagtagaa ttgacgattg
      241 gggttatata aaatggtgcg atcttaaaat atctgacttt gtgctagttt aagttcattt
      301 gtgaatggaa gacatgtaac gctttaacta ttcagaatat cagttccaag tgaagatgga
      361 ggtagcaaaa gtaagactaa taaccaaatc aataaaaatc ttatacaata cttatattaa
      421 cattgcaaag agtttcaagg tttttcttct gagttttgaa agaaattata gggaacaaaa
      481 ggaataaaga ggcatcaatt cgatcactca gagctacaaa acaatgggac taaaacgcaa
      541 aacgaaagcg gttttttttt ttgaaacgca gacgtaagta aaaacccaga gagtttgtca
      601 cacacaagtg catcatagaa acgaaaacaa aaagggaaat gaaacaaaca aatggagaag
      661 caaatataaa agaaaaaaag aaactttgat cccattcata aaaccccagc tttttaagcc
      721 tttgatcttg agagcaagcc gagaccactc atcgcccaat cgaattcccg cggccgccat
      781 ggcggccggg agcatgcgac gtcgggccca attcgcccta tagtgagtcg tattacaatt
      841 cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc
      901 gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc
      961 gcccttccca acagttgcgc agcctgaatg gcgaatggac gcgccctgta gcggcgcatt
     1021 aag
//
