LOCUS       sequence-138020-         847 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Contains dCas9 from S. pyogenes under constitutive promoter. Suicide
            vector inserts into psba1 site of Synechocystis. Carries 
            spectinomycin resist. Recommend E. coli Copy cutter for 
            propogation..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 847)
  AUTHORS   Yao L, Cengic I, Anfelt J, Hudson EP
  TITLE     Multiple Gene Repression in Cyanobacteria Using CRISPRi.
  JOURNAL   ACS Synth Biol. 2015 Dec 28.
  PUBMED    26689101
REFERENCE   2  (bases 1 to 847)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Dec 28."
COMMENT     Sequence Label: pMD19T-psba1-Ppsba2-dCas9-SpR
FEATURES             Location/Qualifiers
     source          1..847
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gatggtactg aggaattatt ggtgaaacta aatcgtgaag atttgctgcg caagcaacgg
       61 acctttgaca acggctctat tccccatcaa attcacttgg gtgagctgca tgctattttg
      121 agaagacaag aagactttta tccattttta aaagacaatc gtgagaagat tgaaaaaatc
      181 ttgacttttc gcattcctta ttatgttggt ccattggcgc gtggcaatag tcgttttgca
      241 tggatgactc ggaagtctga agaaacaatt accccatgga attttgaaga agttgtcgat
      301 aaaggtgctt cagctcaatc atttattgaa cgcatgacaa actttgataa aaatcttcca
      361 aatgaaaaag tactaccaaa acatagtttg ctttatgagt attttacggt ttataacgaa
      421 ttgacaaagg tcaaatatgt tactgaagga atgcgaaaac cagcatttct ttcaggtgaa
      481 cagaagaaag ccattgttga tttactcttc aaaacaaatc gaaaagtaac cgttaagcaa
      541 ttaaaagaag attatttcaa aaaaatagaa tgttttgata gtgttgaaat ttcaggagtt
      601 gaagatagat ttaatgcttc attaggtacc taccatgatt tgctaaaaat tattaaagat
      661 aaagattttt tggataatga agaaaatgaa gatatcttag aggatattgt tttaacattg
      721 accttatttg aagataggga gatgattgag gaaagactta aaacatatgc tcacctcttt
      781 gatgataagg tgatgaaaca gcttaaacgt cgccgttata ctggttgggg acgtttgtct
      841 cgaaaat
//
