LOCUS       sequence-138021-        3997 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Provides the CDS of the Renilla protein as a level 0 GoldenBraid 
            part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3997)
  AUTHORS   Sarrion-Perdigones A, Vazquez-Vilar M, Palaci J, Castelijns B, 
            Forment J, Ziarsolo P, Blanca J, Granell A, Orzaez D
  TITLE     GoldenBraid 2.0: a comprehensive DNA assembly framework for plant 
            synthetic biology.
  JOURNAL   Plant Physiol. 2013 Jul;162(3):1618-31.
  PUBMED    23669743
REFERENCE   2  (bases 1 to 3997)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2013-07"; volume: "162(3)"; pages: "1618-31"
COMMENT     Sequence Label: pRenilla (GB0056)
FEATURES             Location/Qualifiers
     source          1..3997
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(12..947)
                     /codon_start=1
                     /product="luciferase from the anthozoan coelenterate 
                     Renilla reniformis (sea pansy)"
                     /label=Rluc
                     /translation="MTSKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     promoter        complement(1047..1065)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1048..1065)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(1083..1099)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1083..1099)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1096..1118)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1107..1123
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1131..1137,1138..1155,1156..1161))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1176..1197
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1314..1331)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1485..2073)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1565..1584)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2244..3035,3036..3104))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2867..2886
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3105..3209)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3287..3742)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3424..3445)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3636..3655
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3868..3890
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3882..3899
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3883..3899
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3906..3925
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3906..3924
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      3955..3964
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      3959..3968
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ctcgaagctc attattgttc atttttgaga actcgctcaa cgaacgattt gatatatttt
       61 cccatttcat caggtgcatc ttcttgcgaa aaatgaagac cttttacttt gacaaattca
      121 gtattaggaa acttcttggc accttcaaca atagcattgg aaaagaatcc tgggtccgat
      181 tcaataaaca tttttggtaa atcatcactt gcacgtagat aagcattata attcctaaca
      241 atttgtacaa cgtcaggttt accacctttt actaacggga tttcacgagg ccatgataat
      301 gttggacgac gaacttcacc tttctctttg aatggttcaa gatatgctgc aaattcttct
      361 ggttctaact ttctcatgat ttttgatggc aacatggttt ccacgaagaa gttattctcc
      421 aaaaccattt tttctccttc ttcagatttg atcaacgcaa tatcttcttc aatatcaggc
      481 cattcatccc atgattcaat cacatctact acactttcag cgtgaactat tgctttgatc
      541 ttatcttgat gctcatagct ataatgaaat gccaaacaag caccccaatc atggccgaca
      601 aaaatgatct tctttggtaa attaagaagt tcaaaccatg cagtaagata tttgtaatga
      661 tcaagtaacc tataagaacc attaccagat ttgcctgatt tgcccatacc aataaggtct
      721 ggtataatac accgcgctac tggctcaata tgtggcacaa catgtcgcca taaataagaa
      781 gaggccgcgt taccatgtaa aaaaataaca gcattttctg catgtttttc tgaatcataa
      841 taattaataa atgaatcaag aacattcatt tgtttacatc tggcccacca ctgcggacca
      901 gttatcatcc gtttcctttg ttctggatca taaactttcg aagtcattcg agaccactca
      961 tcgcccaatc actagtgaat tcgcggccgc ctgcaggtcg accatatggg agagctccca
     1021 acgcgttgga tgcatagctt gagtattcta tagtgtcacc taaatagctt ggcgtaatca
     1081 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
     1141 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
     1201 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
     1261 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     1321 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     1381 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     1441 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     1501 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     1561 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     1621 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     1681 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     1741 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     1801 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     1861 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     1921 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     1981 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     2041 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     2101 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     2161 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     2221 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     2281 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     2341 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgcgaccc acgctcaccg
     2401 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     2461 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     2521 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     2581 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     2641 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     2701 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     2761 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     2821 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     2881 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     2941 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     3001 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     3061 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     3121 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     3181 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgatgcg
     3241 gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggaaat tgtaagcgtt
     3301 aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag
     3361 gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt
     3421 gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga
     3481 aaaaccgtct atcagggcgc tggcccacta cgtgaaccat caccctaatc aagttttttg
     3541 gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct
     3601 tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc
     3661 gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt
     3721 aatgcgccgc tacagggcgc gtccattcgc cattcaggct gcgcaactgt tgggaagggc
     3781 gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc
     3841 gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg
     3901 aattgtaata cgactcacta tagggcgaat tgggcccgac gtcgcatgct cccggccgcc
     3961 atggcggccg cgggaattcg attgggcgat gagtggt
//
