LOCUS       sequence-138022-        1048 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Provides the CDS of the Renilla protein as a level 0 GoldenBraid 
            part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1048)
  AUTHORS   Sarrion-Perdigones A, Vazquez-Vilar M, Palaci J, Castelijns B, 
            Forment J, Ziarsolo P, Blanca J, Granell A, Orzaez D
  TITLE     GoldenBraid 2.0: a comprehensive DNA assembly framework for plant 
            synthetic biology.
  JOURNAL   Plant Physiol. 2013 Jul;162(3):1618-31.
  PUBMED    23669743
REFERENCE   2  (bases 1 to 1048)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2013-07"; volume: "162(3)"; pages: "1618-31"
COMMENT     Sequence Label: pRenilla (GB0056)
FEATURES             Location/Qualifiers
     source          1..1048
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      32..41
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      36..45
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(86..1021)
                     /codon_start=1
                     /product="luciferase from the anthozoan coelenterate 
                     Renilla reniformis (sea pansy)"
                     /label=Rluc
                     /translation="MTSKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
ORIGIN
        1 ggcgaattgg gcccgacgtc gcatgctccc ggccgccatg gcggccgcgg gaattcgatt
       61 gggcgatgag tggtctcgaa gctcattatt gttcattttt gagaactcgc tcaacgaacg
      121 atttgatata ttttcccatt tcatcaggtg catcttcttg cgaaaaatga agacctttta
      181 ctttgacaaa ttcagtatta ggaaacttct tggcaccttc aacaatagca ttggaaaaga
      241 atcctgggtc cgattcaata aacatttttg gtaaatcatc acttgcacgt agataagcat
      301 tataattcct aacaatttgt acaacgtcag gtttaccacc ttttactaac gggatttcac
      361 gaggccatga taatgttgga cgacgaactt cacctttctc tttgaatggt tcaagatatg
      421 ctgcaaattc ttctggttct aactttctca tgatttttga tggcaacatg gtttccacga
      481 agaagttatt ctccaaaacc attttttctc cttcttcaga tttgatcaac gcaatatctt
      541 cttcaatatc aggccattca tcccatgatt caatcacatc tactacactt tcagcgtgaa
      601 ctattgcttt gatcttatct tgatgctcat agctataatg aaatgccaaa caagcacccc
      661 aatcatggcc gacaaaaatg atcttctttg gtaaattaag aagttcaaac catgcagtaa
      721 gatatttgta atgatcaagt aacctataag aaccattacc agatttgcct gatttgccca
      781 taccaataag gtctggtata atacaccgcg ctactggctc aatatgtggc acaacatgtc
      841 gccataaata agaagaggcc gcgttaccat gtaaaaaaat aacagcattt tctgcatgtt
      901 tttctgaatc ataataatta ataaatgaat caagaacatt catttgttta catctggccc
      961 accactgcgg accagttatc atccgtttcc tttgttctgg atcataaact ttcgaagtca
     1021 ttcgagacca ctcatcgccc aatcacta
//
