LOCUS       sequence-138024-        3088 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  entry vector (L4-R1) containing the Notch responsive tp1 element 
            upstream of a basal promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3088)
  AUTHORS   Quillien A, Moore JC, Shin M, Siekmann AF, Smith T, Pan L, Moens CB,
            Parsons MJ, Lawson ND
  TITLE     Distinct Notch signaling outputs pattern the developing arterial 
            system.
  JOURNAL   Development. 2014 Apr;141(7):1544-52. doi: 10.1242/dev.099986. Epub 
            2014 Mar 5.
  PUBMED    24598161
REFERENCE   2  (bases 1 to 3088)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1242/dev.099986"; journalName: "Development"; date: "2014-04"; 
            volume: "141"; issue: "7"; pages: "1544-52"
COMMENT     Sequence Label: 502 p5E-tp1
FEATURES             Location/Qualifiers
     source          1..3088
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    593..688
                     /gene="mutant version of attL"
                     /label=attL4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    1133..1257
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1329..1348)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1330..1348)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1353..1369)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1353..1369)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1471..1490)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1482..2291
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1549..1568)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2438..3026
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2927..2946
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccgc gttaacgcta ccatggagct ccaaataatg
      601 attttatttt gactgatagt gacctgttcg ttgcaacaaa ttgataagca atgctttttt
      661 ataatgccaa ctttgtatag aaaagttgtg caggtcgact ctagaggatc ccgactcatg
      721 ggaaaatggg cggaagggca ccgtgggaaa atagtagatc ccgactcgtg ggaaaatggg
      781 cggaagggca ccgtgggaaa atagtagatc ccgactcgtg ggaaaatggg cggaagggca
      841 ccgtgggaaa atagtagatc ccgactcatg ggaaaatggg cggaagggca ccgtgggaaa
      901 atagtagatc ccgactcgtg ggaaaatggg cggaagggca ccgtgggaaa atagtagatc
      961 ccgactcgtg ggaaaatggg cggaagggca ccgtgggaaa atagtagatc cactgtatgc
     1021 atcttgggca taaaaggcag atcacttcag cttctgctta cacttgcttt tgacacaact
     1081 gtgtttactt gcaatccctc gaatcgctag catcgattcg aattcaagga tcacaagttt
     1141 gtacaaaaaa gttgaacgag aaacgtaaaa tgatataaat atcaatatat taaattagat
     1201 tttgcataaa aaacagacta cataatactg taaaacacaa catatgcagt cactatgaac
     1261 caactactta gatggtatta gtgacctgta gaattcgagc tctagagctg cagggcggcc
     1321 gcgatatccc ctatagtgag tcgtattaca tggtcatagc tgtttcctgg cagctctggc
     1381 ccgtgtctca aaatctctga tgttacattg cacaagataa aaatatatca tcatgaacaa
     1441 taaaactgtc tgcttacata aacagtaata caaggggtgt tatgagccat attcaacggg
     1501 aaacgtcgag gccgcgatta aattccaaca tggatgctga tttatatggg tataaatggg
     1561 ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg cttgtatggg aagcccgatg
     1621 cgccagagtt gtttctgaaa catggcaaag gtagcgttgc caatgatgtt acagatgaga
     1681 tggtcagact aaactggctg acggaattta tgcctcttcc gaccatcaag cattttatcc
     1741 gtactcctga tgatgcatgg ttactcacca ctgcgatccc cggaaaaaca gcattccagg
     1801 tattagaaga atatcctgat tcaggtgaaa atattgttga tgcgctggca gtgttcctgc
     1861 gccggttgca ttcgattcct gtttgtaatt gtccttttaa cagcgatcgc gtatttcgtc
     1921 tcgctcaggc gcaatcacga atgaataacg gtttggttga tgcgagtgat tttgatgacg
     1981 agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat gcataaactt ttgccattct
     2041 caccggattc agtcgtcact catggtgatt tctcacttga taaccttatt tttgacgagg
     2101 ggaaattaat aggttgtatt gatgttggac gagtcggaat cgcagaccga taccaggatc
     2161 ttgccatcct atggaactgc ctcggtgagt tttctccttc attacagaaa cggctttttc
     2221 aaaaatatgg tattgataat cctgatatga ataaattgca gtttcatttg atgctcgatg
     2281 agtttttcta atcagaattg gttaattggt tgtaacactg gcagagcatt acgctgactt
     2341 gacgggacgg cgcaagctca tgaccaaaat cccttaacgt gagttacgcg tcgttccact
     2401 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     2461 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     2521 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     2581 ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     2641 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     2701 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     2761 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     2821 agcgtgagca ttgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     2881 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     2941 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     3001 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     3061 ccttttgctg gccttttgct cacatgtt
//
