LOCUS       sequence-138025-         732 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  entry vector (L4-R1) containing the Notch responsive tp1 element 
            upstream of a basal promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 732)
  AUTHORS   Quillien A, Moore JC, Shin M, Siekmann AF, Smith T, Pan L, Moens CB,
            Parsons MJ, Lawson ND
  TITLE     Distinct Notch signaling outputs pattern the developing arterial 
            system.
  JOURNAL   Development. 2014 Apr;141(7):1544-52. doi: 10.1242/dev.099986. Epub 
            2014 Mar 5.
  PUBMED    24598161
REFERENCE   2  (bases 1 to 732)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1242/dev.099986"; journalName: "Development"; date: "2014-04"; 
            volume: "141"; issue: "7"; pages: "1544-52"
COMMENT     Sequence Label: 502 p5E-tp1
FEATURES             Location/Qualifiers
     source          1..732
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     17..36
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     138..154
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     138..154
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     159..178
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        159..177
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(250..374)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 acgtttcccg ttgaatatgg ctcataacac cccttgtatt actgtttatg taagcagaca
       61 gttttattgt tcatgatgat atatttttat cttgtgcaat gtaacatcag agattttgag
      121 acacgggcca gagctgccag gaaacagcta tgaccatgta atacgactca ctatagggga
      181 tatcgcggcc gccctgcagc tctagagctc gaattctaca ggtcactaat accatctaag
      241 tagttggttc atagtgactg catatgttgt gttttacagt attatgtagt ctgtttttta
      301 tgcaaaatct aatttaatat attgatattt atatcatttt acgtttctcg ttcaactttt
      361 ttgtacaaac ttgtgatcct tgaattcgaa tcgatgctag cgattcgagg gattgcaagt
      421 aaacacagtt gtgtcaaaag caagtgtaag cagaagctga agtgatctgc cttttatgcc
      481 caagatgcat acagtggatc tactattttc ccacggtgcc cttccgccca ttttcccacg
      541 agtcgggatc tactattttc ccacggtgcc cttccgccca ttttcccacg agtcgggatc
      601 tactattttc ccacggtgcc cttccgccca ttttcccatg agtcgggatc tactattttc
      661 ccacggtgcc cttccgccca ttttcccacg agtcgggatc tactattttc ccacggtgcc
      721 cttccgccca tt
//
