LOCUS       sequence-138036-         656 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Contains dCas9 from S. pyogenes under constitutive promoter. Suicide
            vector inserts into psba1 site of Synechocystis. Carries 
            spectinomycin resist. Recommend E. coli Copy cutter for 
            propogation..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Yao L, Cengic I, Anfelt J, Hudson EP
  TITLE     Multiple Gene Repression in Cyanobacteria Using CRISPRi.
  JOURNAL   ACS Synth Biol. 2015 Dec 28.
  PUBMED    26689101
REFERENCE   2  (bases 1 to 656)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Dec 28."
COMMENT     Sequence Label: pMD19T-psba1-Ppsba2-dCas9-SpR
FEATURES             Location/Qualifiers
     source          1..656
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    476..497
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 agagccccta taagattttt tttgatactg tggcggtctg tatttcccag aaccttgaac
       61 tttttagacg gaaccttata ttcatcagtg atcaccgccc atccgacgct atttgtgccg
      121 atagctaagc ctattgagta tttcttatcc atctagtttg gttataattc cttagttcag
      181 attggaactg actaaactta gtctaaagga ttaatgagag ttttgtaaag ctttgtaaca
      241 ggaagttaat atacacgaag cttatagatg acataagttt tactttcttg taattgtcgt
      301 tttttccatg ggtaaacaga gggcgttttg ggggttgaga agagtttacc tggcggtttt
      361 tcgtgcgcct ttaccatagt tctgggctgt gtagaggggc gacacaactg attttcaggg
      421 acaatggaga tgaaataatg aaaaataatg aaattgatca ctgggccttg cacgcctcta
      481 gaagcggccg cgaattctgg tttctcagat tgcagttggt ttccaaaggt aaaaaaaaga
      541 ttttaatagg aaaaaataaa tcattaaaaa ataaaaattt ttctcgtaaa tgccagatca
      601 agccattttt ccccgatcgc cccattggcc aaaggaacaa aatactgcct ggttgt
//
