LOCUS       sequence-138038-         894 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Contains dCas9 from S. pyogenes under constitutive promoter. Suicide
            vector inserts into psba1 site of Synechocystis. Carries 
            spectinomycin resist. Recommend E. coli Copy cutter for 
            propogation..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 894)
  AUTHORS   Yao L, Cengic I, Anfelt J, Hudson EP
  TITLE     Multiple Gene Repression in Cyanobacteria Using CRISPRi.
  JOURNAL   ACS Synth Biol. 2015 Dec 28.
  PUBMED    26689101
REFERENCE   2  (bases 1 to 894)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Dec 28."
COMMENT     Sequence Label: pMD19T-psba1-Ppsba2-dCas9-SpR
FEATURES             Location/Qualifiers
     source          1..894
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      25..96
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      112..139
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
ORIGIN
        1 atttgtaata ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc
       61 tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct
      121 tcgggtgggc ctttctgcgt ttatatacta gagccctcgg cttgaacgaa ttgttagaca
      181 ttatttgccg actaccttgg tgatctcgcc tttcacgtag tggacaaatt cttccaactg
      241 atctgcgcgc gaggccaagc gatcttcttc ttgtccaaga taagcctgtc tagcttcaag
      301 tatgacgggc tgatactggg ccggcaggcg ctccattgcc cagtcggcag cgacatcctt
      361 cggcgcgatt ttgccggtta ctgcgctgta ccaaatgcgg gacaacgtaa gcactacatt
      421 tcgctcatcg ccagcccagt cgggcggcga gttccatagc gttaaggttt catttagcgc
      481 ctcaaataga tcctgttcag gaaccggatc aaagagttcc tccgccgctg gacctaccaa
      541 ggcaacgcta tgttctcttg cttttgtcag caagatagcc agatcaatgt cgatcgtggc
      601 tggctcgaag atacctgcaa gaatgtcatt gcgctgccat tctccaaatt gcagttcgcg
      661 cttagctgga taacgccacg gaatgatgtc gtcgtgcaca acaatggtga cttctacagc
      721 gcggagaatc tcgctctctc caggggaagc cgaagtttcc aaaaggtcgt tgatcaaagc
      781 tcgccgcgtt gtttcatcaa gccttacggt caccgtaacc agcaaatcaa tatcactgtg
      841 tggcttcagg ccgccatcca ctgcggagcc gtacaaatgt acggccagca acgt
//
