LOCUS       sequence-138039-         759 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Escherichia coli phosphoribosylpyrophosphate synthetase 
            (PrsA), N-terminal His6 tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 759)
  AUTHORS   Schultheisz HL, Szymczyna BR, Scott LG, Williamson JR
  TITLE     Pathway engineered enzymatic de novo purine nucleotide synthesis.
  JOURNAL   ACS Chem Biol. 2008 Aug 15;3(8):499-511. doi: 10.1021/cb800066p.
  PUBMED    18707057
REFERENCE   2  (bases 1 to 759)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "ACS Chem Biol"; date: "2008-08-15- 15"; volume: "3"; issue: "8"; 
            pages: "499-511"
COMMENT     Sequence Label: pET22-HT/prsA
FEATURES             Location/Qualifiers
     source          1..759
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             20..37
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             41..61
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 agaaggagat atacatatgc atcatcatca tcatcacggc gaaaacctgt attttcaggg
       61 cggatccgtg cctgatatga agctttttgc tggtaacgcc accccggaac tagcacaacg
      121 tattgccaac cgcctgtaca cttcactcgg cgacgccgct gtaggtcgct ttagcgatgg
      181 cgaagtcagc gtacaaatta atgaaaatgt acgcggtggt gatattttca tcatccagtc
      241 cacttgtgcc cctactaacg acaacctgat ggaattagtc gttatggttg atgccctgcg
      301 tcgtgcttcc gcaggtcgta tcaccgctgt tatcccctac tttggctatg cgcgccagga
      361 ccgtcgcgtc cgttccgctc gtgtaccaat cactgcgaaa gtggttgcag acttcctctc
      421 cagcgtcggt gttgaccgtg tgctgacagt ggatctgcac gctgaacaga ttcagggttt
      481 cttcgacgtt ccggttgata acgtatttgg tagcccgatc ctgctggaag acatgctgca
      541 gctgaatctg gataacccaa ttgtggtttc tccggacatc ggcggcgttg tgcgtgcccg
      601 cgctatcgct aagctgctga acgataccga tatggcaatc atcgacaaac gtcgtccgcg
      661 tgcgaacgtt tcccaggtga tgcatatcat cggtgacgtt gcaggtcgtg actgcgtact
      721 ggtcgatgat atgatcgaca ctggcggtac gctgtgtaa
//
