LOCUS       sequence-138041-         769 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  promoterless Tol2 vector with 6xmyc tag upstream of and in frame 
            with attR1/attR2 cassette and late SV40 polyadenylation signal.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 769)
  AUTHORS   Villefranc JA, Amigo J, Lawson ND
  TITLE     Gateway compatible vectors for analysis of gene function in the 
            zebrafish.
  JOURNAL   Dev Dyn. 2007 Nov . 236(11):3077-87.
  PUBMED    17948311
REFERENCE   2  (bases 1 to 769)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Dyn. 
            2007 Nov . 236(11):3077-87."
COMMENT     Sequence Label: 252 pTol2-MTDest
FEATURES             Location/Qualifiers
     source          1..769
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    join(77..88,89..251)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     CDS             647..676
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             686..715
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             725..754
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 aagacgtgaa tatctgttca gacacccata tccactctgt tccacacagg tcagaggttt
       61 gtccaggagt tcttgacaga ggtgtaaaaa gtactcaaaa attttactca agtgaaagta
      121 caagtactta gggaaaattt tactcaatta aaagtaaaag tatctggcta gaatcttact
      181 tgagtaaaag taaaaaagta ctccattaaa attgtacttg agtattaagg aagtaaaagt
      241 aaaagcaaga aagaaaacta gagattcttg tttaagcttt taatctcaaa aaacattaaa
      301 tgaaatgcat acaaggtttt atcctgcttt agaactgttt gtatttaatt atcaaactat
      361 aagacagaca atctaatgcc agtacacgct actcaaagtt gtaaaacctc agatttaact
      421 tcagtagaag ctgattctca aaattgttag tgtcaagcct agctcttttg gggctgaaaa
      481 gcaatcctgc agtgctgaaa agcctctcac aggcagccga tgcgggaaga ggtgtattag
      541 tcttgataga gaggctgcaa atagcaggaa acgtgagcag agactccctg gtgtctgaaa
      601 cacaggccag atgggccgat atcgctagca tcgatttaaa gctatggagc aaaagctcat
      661 ttctgaagag gacttgaatg aaatggagca aaagctcatt tctgaagagg acttgaatga
      721 aatggagcaa aagctcattt ctgaagagga cttgaatgaa atggagcaa
//
