LOCUS       sequence-138057-         751 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Escherichia coli phosphoribosylpyrophosphate synthetase 
            (PrsA), N-terminal His6 tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 751)
  AUTHORS   Schultheisz HL, Szymczyna BR, Scott LG, Williamson JR
  TITLE     Pathway engineered enzymatic de novo purine nucleotide synthesis.
  JOURNAL   ACS Chem Biol. 2008 Aug 15;3(8):499-511. doi: 10.1021/cb800066p.
  PUBMED    18707057
REFERENCE   2  (bases 1 to 751)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "ACS Chem Biol"; date: "2008-08-15- 15"; volume: "3"; issue: "8"; 
            pages: "499-511"
COMMENT     Sequence Label: pET22-HT/prsA
FEATURES             Location/Qualifiers
     source          1..751
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(14..31)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 cagccggatc tcagtggtgg tggtggtggt gctcgagtgc ggccgcaagc ttgtcgacgg
       61 agctcgaatt cttagtgttc gaacatggca gagatcgatt cttcgttgct gatacgacga
      121 atcgcttcgg ccagcatacc tgacagggtc agagtacgca cgttcggcag tgatttgatt
      181 tcatcgctca gcggaatggt atcgcagaca acgacttcat caattacaga gttacgcagg
      241 ttgttcgccg cgttgccaga gaagatcggg tgagtcgcgt acgcaaatac acgtttagca
      301 ccacgttctt tcagagcttc agcagcttta cacagcgtac cgccagtgtc gatcatatca
      361 tcgaccagta cgcagtcacg acctgcaacg tcaccgatga tatgcatcac ctgggaaacg
      421 ttcgcacgcg gacgacgttt gtcgatgatt gccatatcgg tatcgttcag cagcttagcg
      481 atagcgcggg cacgcacaac gccgccgatg tccggagaaa ccacaattgg gttatccaga
      541 ttcagctgca gcatgtcttc cagcaggatc gggctaccaa atacgttatc aaccggaacg
      601 tcgaagaaac cctgaatctg ttcagcgtgc agatccactg tcagcacacg gtcaacaccg
      661 acgctggaga ggaagtctgc aaccactttc gcagtgattg gtacacgagc ggaacggacg
      721 cgacggtcct ggcgcgcata gccaaagtag g
//
