LOCUS       sequence-138058-         464 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAV zinc finger donor vector for genomic targeting.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 464)
  AUTHORS   Hockemeyer D, Soldner F, Beard C, Gao Q, Mitalipova M, Dekelver RC, 
            Katibah GE, Amora R, Boydston EA, Zeitler B, Meng X, Miller JC, 
            Zhang L, Rebar EJ, Gregory PD, Urnov FD, Jaenisch R
  TITLE     Efficient targeting of expressed and silent genes in human ESCs and 
            iPSCs using zinc-finger nucleases.
  JOURNAL   Nat Biotechnol. 2009 Sep;27(9):851-7. doi: 10.1038/nbt.1562
  PUBMED    19680244
REFERENCE   2  (bases 1 to 464)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt"; 
            journalName: "Nat Biotechnol"; date: "2009-09"; volume: "27"; issue:
            "9"; pages: "851-7"
COMMENT     Sequence Label: AAV-CAGGS-EGFP
FEATURES             Location/Qualifiers
     source          1..464
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      10..19
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(48..67)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     complement(129..151)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
ORIGIN
        1 ccttgctcac catggtggcg gaattctttg ccaaaatgat gagacagcac aataaccagc
       61 acgttgccca ggagctgtag gaaaaagaag aaggcatgaa catggttagc agaggctcta
      121 gagccgccgg tcacacgcca gaagccgaac cccgccctgc cccgtccccc ccgaaggcag
      181 ccgtcccccc gcggacagcc ccgaggctgg agagggagaa ggggacggcg gcgcggcgac
      241 gcacgaaggc cctccccgcc catttccttc ctgccggcgc cgcaccgctt cgccccgcgc
      301 ccgctagagg gggtgcggcg gcgcctccca gatttcggct ccgcacagat ttgggacaaa
      361 ggaagtccct gcgccctctc gcacgattac cataaaaggc aatggctgcg gctcgccgcg
      421 cctcgacagc cgccggcgct ccgggggccg ccgcgcccct cccc
//
