LOCUS       sequence-138059-         675 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  promoterless Tol2 vector with attR1/attR2 cassette and late SV40 
            polyadenylation signal.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 675)
  AUTHORS   Villefranc JA, Amigo J, Lawson ND
  TITLE     Gateway compatible vectors for analysis of gene function in the 
            zebrafish.
  JOURNAL   Dev Dyn. 2007 Nov . 236(11):3077-87.
  PUBMED    17948311
REFERENCE   2  (bases 1 to 675)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Dyn. 
            2007 Nov . 236(11):3077-87."
COMMENT     Sequence Label: 251 pTol2Dest
FEATURES             Location/Qualifiers
     source          1..675
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     31..47
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     31..47
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     misc_feature    join(81..92,93..280)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
ORIGIN
        1 aagctggagc tccaccgcgg tggcggccgc tctagaacta gtggatctgc tgggcttgct
       61 gaaggtaggg ggtcaagaac cagaggtgta aagtacttga gtaattttac ttgattactg
      121 tacttaagta ttatttttgg ggatttttac tttacttgag tacaattaaa aatcaatact
      181 tttactttta cttaattaca tttttttaga aaaaaaagta ctttttactc cttacaattt
      241 tatttacagt caaaaagtac ttattttttg gagatcactt cattctattt tcccttgcta
      301 ttaccaaacc aattgaattg cgctgatgcc cagtttaatt taaatgttat ttattctgcc
      361 tatgaaaatc gttttcacat tatatgaaat tggtcagaca tgttcattgg tcctttggaa
      421 gtgacgtcat gtcacatcta ttaccacaat gcacagcacc ttgacctgga aattagggaa
      481 attataacag tcaatcagtg gaagaaaatg gaggaagtat gtgattcatc agcagctgcg
      541 agcagcacag tccaaaatca gccacaggat caagagcacc cgtggccgta tcttcgcgaa
      601 ttcttttctt taagtggtgt aaataaagat tcattcaaga tgaaatgtgt cctctgtctc
      661 ccgcttaata aagaa
//
